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Plasmid#105235PurposeTn7 integrating plasmid with S. pasteurianus dCas9 expressed from the Plac promoter for expression in P. aeruginosaDepositorInsertS. pasteurianus dCas9
UseCRISPR and Synthetic BiologyTagsHAExpressionBacterialMutationD10A, H599APromoterAvailable sinceAug. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM220 (pUC18T-miniTn7T-gm-rhaSR-PrhaBAD)
Plasmid#110559PurposeminiTn7 delivery plasmid with rhaSR-PrhaBAD inducible promoterDepositorInsertrhaSR-PrhaBAD
UseTagsExpressionBacterialMutationPromoterrhaSR-PrhaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM101(pUC18T-miniTn7T-gm-lacIq-Ptac)
Plasmid#110558PurposeminiTn7 delivery plasmid with lacIq-Ptac inducible promoterDepositorInsertlacIq-Ptac
UseTagsExpressionBacterialMutationPromoterlacIq-PtacAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJM100 (pUC18T-miniTn7T-gm-araC-ParaBAD)
Plasmid#110554PurposeminiTn7 delivery plasmid with araC-ParaBAD inducible promoterDepositorInsertaraC-ParaBAD
UseTagsExpressionBacterialMutationPromoterAraC-ParaBADAvailable sinceMay 23, 2018AvailabilityAcademic Institutions and Nonprofits only -
PEP109E
Plasmid#97395Purposebinary construct of cytosolic mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagscytosolExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b-17X-PTDH
Plasmid#166786PurposePhosphite dehydrogenase for NAD(P)H regenerationDepositorInsertPhosphite dehydroenase
UseTagsHis6ExpressionBacterialMutationA196R, T201S, A328T, E352N, and C356D- please see…PromoterT7Available sinceMay 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
PEP110E
Plasmid#97396Purposebinary construct of nuclear mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsNucleus targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP104E
Plasmid#97390Purposebinary construct of mitochondria-targeted sfGFP1-10 for plant expressionDepositorInsertsfGFP1-10OPT
UseTagsmitochondria targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPdX
Plasmid#85084PurposeExpresses putidaredoxin in E. coliDepositorInsertPutidaredoxin
UseTagsHis6 tagExpressionBacterialMutationchanged Cysteine 73 to Serine and Cysteine 85 to …PromoterT7 promoterAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENO1-iRFP-NATr
Plasmid#129731PurposeIntegration plasmid to overexpress iRFP in Candida albicansDepositorInsertiRFP-Candida albicans codon-optimized
UseTagsExpressionYeastMutationPromoterENO1 from Candida albicansAvailable sinceAug. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
miniTn7(Gm)PA1/04/03-eyfp-a
Plasmid#111620PurposeeYFP Fluorescent tagging of bacteria at their Tn7-siteDepositorInsertPA1/04/03-eyfp
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCK01_PalkS_alkS
Plasmid#166502PurposeExpression of AlkS transcriptional regulator with L401G mutation. Sensor input plasmid for detection of n-butanol and branched-chain higher alcohols.DepositorInsertalkS
UseSynthetic BiologyTagsExpressionBacterialMutationL401GPromoterPalkSAvailable sinceNov. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pNVLTv2-pvdIJD-Ptrc-base
Plasmid#169243PurposeSuicide vector for integrating expression cassette in P. putida (IPTG induction)DepositorInsertlacI
UseTagsExpressionBacterialMutationPromoterlacIqAvailable sinceJune 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMA3174
Plasmid#46880PurposeLentiviral vector for the dual control of expression through Tet-On promoter and C-terminal destabilizing domainDepositorTypeEmpty backboneUseLentiviralTagsFFKBP12 E31G, R71G, K105E and mycExpressionMutationPromoterTRE-TightAvailable sinceAug. 20, 2013AvailabilityAcademic Institutions and Nonprofits only -
PEP112E
Plasmid#97398Purposebinary construct of mitochondria-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsMitochondria targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP111E
Plasmid#97397Purposebinary construct of plastid-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsPlastid targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPDK3181
Plasmid#97403Purposebinary construct of ER-targeted sfCherry1-10 for plant expressionDepositorInsertER-sfCherry1-10
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
TR-Csy4
Plasmid#80601PurposeExpresses Csy4 from the CBA promoter. Cloned into an Adeno-Associated Virus backbone for packaging into AAV.DepositorInsertCsy4
UseAAVTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
PEP115E
Plasmid#97401Purposebinary construct of Golgi-targeted mCherry with sfGFP11 tag for plant expressionDepositorInsertmCherry-sfGFP11
UseTagsGolgi targetExpressionPlantMutationPromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
SPDK3182
Plasmid#97404Purposebinary construct of ER-targeted sfYFP1-10 for plant expressionDepositorInsertER-sfYFP1-10
UseTagsER targetExpressionPlantMutationPromoterAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only