Showing: 341 - 360 of 1088 results
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Plasmid#145030PurposepiggyBac vector for expressing a Turquoise calcium sensor (GECI) that reports with lifetime and intensity changesDepositorInsert3xnls-Tq-Ca-FLITS
UseTags3xnls-Tq-Ca-FLITSExpressionMammalianMutationPromoterCMVAvailabilityAcademic Institutions and Nonprofits only -
AAV-PB-SRT-tdTomato
Plasmid#154889PurposepiggyBac self-reporting transposon with tdTomato marker in AAV backboneDepositorInsertpiggyBac SRT
UseAdenoviralTagsExpressionMammalianMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro
Plasmid#183409PurposepiggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cellsDepositorInsertdCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro
Plasmid#183410PurposepiggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cellsDepositorInsertKRAB-dCas9-ecDHFR
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterTRE3GAvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo
Plasmid#183411PurposepiggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.DepositorInsertTet-on 3G
UseCRISPR and Synthetic Biology; PiggybacTagsExpressionMammalianMutationPromoterEF1AAvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-Dnmt3a2-Ires2-mCherry-SV40
Plasmid#186969PurposePiggyBac vector encoding mouse Dnmt3a2 with Ires2-mCherry fluorescent marker for expression in mammalian cells.DepositorInsertDnmt3a2 (Dnmt3a Mouse, Synthetic)
UsePiggybacTagsFlagExpressionMammalianMutationIsoform 2 (residues 220–908)PromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pAAV-H2B-tdTomato-SRT
Plasmid#203393PurposeAAV transfer plasmid containing H2B fused piggyBac self-reporting transposon with tdTomato marker for mammalian calling cardsDepositorInsertH2B fused piggyBac tdTomato self-reporting transposon
UseAAVTags3X SV40 and H2BExpressionMammalianMutationPromoterEf1aAvailabilityAcademic Institutions and Nonprofits only -
pPB-spCas9-pNeo
Plasmid#214120PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB-spCas9-pZeo
Plasmid#214121PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertspCas9
UseCRISPRTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-PlatTET-wpA-pPuroTK
Plasmid#214125PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-TET
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailabilityAcademic Institutions and Nonprofits only -
BII-Sh-BnTW
Plasmid#133365PurposePiggybac vector for shRNA-mediated knockdown, with co-expression with tdTomato-NLSDepositorInserttdTomato-NLS
UseTagsFlag-tagged tdTomatoExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-dCas9-VPR-wpA-pPuroTK
Plasmid#214127PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertdCas9-VPR
UseCRISPRTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Gfi1:FLAG-IRES-EGFP
Plasmid#153945PurposepiggyBac transposon vector with CAG promoter expressing FLAG-tagged Gfi1 and EGFPDepositorInsertGfi1 (Gfi1 Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pPB CAG Cdk6 R31C-IRES-EGFP
Plasmid#153946PurposepiggyBac transposon vector with CAG promoter expressing Cdk6 (R31C mutant) and EGFPDepositorInsertCdk6 (Cdk6 Mouse)
UseTagsExpressionMammalianMutationR31CPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4-IRES-EGFP
Plasmid#153948PurposepiggyBac transposon vector with CAG promoter expressing wild-type SMARCA4 (FLAG-tagged) and EGFPDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4 T910M-IRES-EGFP
Plasmid#153949PurposepiggyBac transposon vector with CAG promoter expressing T910M mutant SMARCA4 (FLAG-tagged) and EGFPDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsFLAGExpressionMammalianMutationT910MPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4-Venus
Plasmid#153950PurposepiggyBac transposon vector with CAG promoter expressing SMARCA4-Venus fusion proteinDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsVenusExpressionMammalianMutationPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
pPB CAG SMARCA4 T910M-Venus
Plasmid#153951PurposepiggyBac transposon vector with CAG promoter expressing SMARCA4 (T910M mutant)-Venus fusion proteinDepositorInsertSMARCA4 (SMARCA4 Human)
UseTagsVenusExpressionMammalianMutationT910MPromoterCAGAvailabilityAcademic Institutions and Nonprofits only -
PB-TRE-dCas9-VPR-pA-3xsgRNA-EF1a-Tet.O-T2A-PuroR-polyA
Plasmid#166693PurposeExpress dCas9-VPR in a doxycyclin-inducable system and 3 sgRNAs targeting the murine Cnga1 promoter. Can be stably integrated into the genome via the PiggyBac Transposon system.DepositorInsertsdCas9-VPR
3x Cnga1 promoter-targeting sgRNAs
UseCRISPRTagsExpressionMammalianMutationPromoterTRE and U6AvailabilityAcademic Institutions and Nonprofits only -
pTRE-SOX17-P2A-Venus-rpEF1a-Zeo
Plasmid#169174PurposepiggyBac transposon to generate a Tet inducible SOX17 expressing cell line. Tet-On inducible when paired with #169175DepositorInsertSOX17-P2A-Venus (SOX17 Human, Synthetic)
UsePiggybac transposonTagsExpressionMammalianMutationPromoterpTREtight (tet-inducible promoter)AvailabilityAcademic Institutions and Nonprofits only
Showing: 341 - 360 of 1088 results