Skip to main content
Addgene
Showing: 341 - 360 of 1134 results

  1. pSARGENT1

    Plasmid
    #216315
    Purpose
    Reporter cassette for SARGENT with piggyBac ITRs and 10x Capture Sequence
    Depositor
    Barak Cohen
    Insert
    tagBFP
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    April 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    216315-plasmid-map-sequence-id-430346

    Enlarge

  2. pPB-CAG-Brainbow4.2-PGK-HygTK

    Plasmid
    #214126
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    Brainbow
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    April 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    214126-plasmid-map-sequence-id-428841

    Enlarge

  3. PB-NEO-GFP-RFP

    Plasmid
    #157952
    Purpose
    seamless gene editing and component of Piggybac transposase system
    Depositor
    Yan-zhen Bi
    Type
    Empty backbone
    Use
    Mouse Targeting; Gene targeting
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Sept. 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    157952-plasmid-map-sequence-id-310668

    Enlarge

  4. BII-ChPtW-iRFP670

    Plasmid
    #133397
    Purpose
    Piggybac vector for constitutive expression of iRFP670-NLS
    Depositor
    Blair Madison
    Insert
    iRFP-670-NLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    N/A
    Promoter
    CMV enhancer and hEf1a (CpG free)
    Available since
    Jan. 21, 2020
    Availability
    Academic Institutions and Nonprofits only
    133397-plasmid-map-sequence-id-269430

    Enlarge

  5. pPB-hVASA-KO-WPRE

    Plasmid
    #214124
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    hKO
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    April 4, 2024
    Availability
    Academic Institutions and Nonprofits only
    214124-plasmid-map-sequence-id-424236

    Enlarge

  6. pPB-cjOCT4-mNG-WPRE

    Plasmid
    #214123
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    mNeonGreen
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    214123-plasmid-map-sequence-id-424234

    Enlarge

  7. pPB-hTHp-Cre-pNeo

    Plasmid
    #214122
    Purpose
    Gene expression by Piggybac transposase in mammalian cells
    Depositor
    Sho Yoshimatsu
    Insert
    Cre
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Codon-optimized for mammalian cells
    Promoter
    Available since
    Feb. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    214122-plasmid-map-sequence-id-423955

    Enlarge

  9. PB-Neo-TetO-Cdk12_Nterm_FlagHa

    Plasmid
    #127179
    Purpose
    N-terminal Flag- HA- tandem epitope tagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vector
    Depositor
    Phil Sharp
    Article
    Dubbury et al Nature. 2018 Dec;564(7734):141-145. doi:
    Insert
    N-terminally Flag- HA- Epitope Tagged Mouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
    Use
    Piggybac transposase vector
    Tags
    Flag- HA- Tandem Epitopes
    Expression
    Mammalian
    Mutation
    Promoter
    TetO Promoter
    Available since
    July 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    127179-plasmid-map-sequence-id-247939

    Enlarge

  10. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid
    #183409
    Purpose
    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183409-plasmid-map-sequence-id-361258

    Enlarge

  11. pPB-Ins-U6p-sgRNAentry-EF1Ap-TetOn3G-IRES-Neo

    Plasmid
    #183411
    Purpose
    piggyBAC-based sgRNA entry (Esp3I) and Tet-On 3G transactivator expression vector. Insert protospacer and transfect with CRISPRa (183409) or CRISPRi (183410) plasmid for inducible epigenome editing.
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    Tet-on 3G
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1A
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183411-plasmid-map-sequence-id-361257

    Enlarge

  12. pPB-Ins-TRE3Gp-KRAB-dCas9-ecDHFR-IRES-GFP-EF1Ap-Puro

    Plasmid
    #183410
    Purpose
    piggyBAC-based doxycycline- and trimethoprim-inducible KRAB-dCas9 CRISPR interference plasmid for enhancer and promoter repression in mammalian cells
    Depositor
    Azim Surani
    Article
    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d
    Insert
    KRAB-dCas9-ecDHFR
    Use
    CRISPR and Synthetic Biology; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE3G
    Available since
    May 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    183410-plasmid-map-sequence-id-361256

    Enlarge

  14. pTETRIS-cargo

    Plasmid
    #126033
    Purpose
    Luciferase reporter construct for the study of cis-repressive activity in long noncoding RNAs. Also confers puromycin resistance.
    Depositor
    Mauro Calabrese
    Article
    Kirk et al Nat Genet. 2018 Oct;50(10):1474-1482. do
    Type
    Empty backbone
    Use
    Luciferase; Piggybac
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE
    Available since
    June 3, 2019
    Availability
    Academic Institutions and Nonprofits only
    126033-plasmid-map-sequence-id-245791

    Enlarge

  17. PB-XL-AncBE4max

    Plasmid
    #136254
    Purpose
    Doxycycline inducible piggyBAC plasmid for mammalian expression of cytosine base editor AncBE4max
    Depositor
    Zhaohui Ye
    Article
    McGrath et al Nat Commun. 2019 Nov 25;10(1):5353. doi:
    Insert
    AncBE4max_GFP
    Use
    CRISPR; Piggybac dna transposon
    Tags
    Expression
    Mammalian
    Mutation
    The AncBE4max_GFP insert was PCR amplified from a…
    Promoter
    TRE3G
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    136254-plasmid-map-sequence-id-270570

    Enlarge

Showing: 341 - 360 of 1134 results