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Plasmid#51252PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 789,805,817,848 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 789,805,817,848 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEF5/FRT/DEST-3xHA/mGli2 P5-6A
Plasmid#51253PurposeEncodes N-3xHA-TEV-mouseGli2-C; amino acids 923,956 mutated to AlaDepositorInsertGli2 (Gli2 Mouse)
UseFlpin systemTags3xHA-TEVExpressionMammalianMutationamino acids 923,956 mutated to AlaPromoterEF1aAvailable sinceFeb. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
p3E-mTagBFP
Plasmid#75175PurposeTagBFP (blue) fluorophore with stop codon for C-terminal fusionsDepositorInsertTagBFP
UseThree prime entry vector for multisite gateway th…TagsExpressionMutationPromotern/aAvailable sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
IO001: pMVP (R4-R3) eGFP
Plasmid#121730PurposepMVP R4-R3 entry plasmid, contains eGFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteGFP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO201: pMVP (R4-R3) mCherry
Plasmid#121732PurposepMVP R4-R3 entry plasmid, contains mCherry for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertmCherry
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IG401: pMVP (L1-R5) RIP
Plasmid#121694PurposepMVP L1-R5 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assemblyDepositorInsertRIP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-stop
Plasmid#113745PurposeContains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.DepositorInsertstop cassette
UseCRISPR; Gateway entry vectorTagsExpressionMutationAdded 3 stop codons to the original sequence; see…PromoterAvailable sinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
HE302: pMVP (L5-L4) RIP
Plasmid#121728PurposepMVP L5-L4 entry plasmid, contains rat Ins1 promoter (RIP) for 4-component MultiSite Gateway Pro assemblyDepositorInsertRIP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO502: pMVP (R4-R3) eCFP
Plasmid#121734PurposepMVP R4-R3 entry plasmid, contains eCFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteCFP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
IO301: pMVP (R4-R3) eYFP
Plasmid#121736PurposepMVP R4-R3 entry plasmid, contains eYFP for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInserteYFP
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HD301: pMVP (R4-R3) βgal
Plasmid#121738PurposepMVP R4-R3 entry plasmid, contains βgal for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertβgal
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
HM001: pMVP (R4-R3) Cre
Plasmid#121740PurposepMVP R4-R3 entry plasmid, contains Cre for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertCre
UseSynthetic Biology; Pmvp gateway entry plasmidTagsExpressionMutationPromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestattB (pCM268)
Plasmid#68313PurposeMultisite Gateway destination vector to create transgenes for phiC31-mediated transgenesis in zebrafishDepositorTypeEmpty backboneUseZebrafish transgenesisTagsExpressionMutationPromoterAvailable sinceAug. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR5'_ubi:loxP-EGFP-loxP
Plasmid#27322DepositorInsertubiquitin:loxP-EGFP-loxP
UseMultisite gateway 5' entry vectorTagsExpressionMutationPromoterAvailable sinceApril 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pENTR eGFPCreERT2 L1-R5
Plasmid#43961DepositorInserteGFP-CreERT2
UseMultisite gateway entr cloneTagsExpressionMutationPromoterAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
p221z-CAS9p-t35s
Plasmid#118385PurposeEntry clone containing CAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p221z-dCAS9p-t35s
Plasmid#118387PurposeEntry clone containing dCAS9p and 35S terminator. Used to make CRISPR construct . For use in plants and compatible with the MultiSite Gateway systemDepositorInsertdCAS9p
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
p221z-AtMIR390a
Plasmid#118388PurposeEntry clone containing AtMIR390a backbone (split by ccdB gene). Used to make artificial microRNA construct. For use in plants and compatible with the MultiSite Gateway system.DepositorInsertAtMIR390a backbone
UseRNAiTagsExpressionMutationPromoterAvailable sinceSept. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRL8
Plasmid#24980DepositorInsertCbr-unc-119
UseMultisite gatewayTagsExpressionMutationPromoterAvailable sinceAug. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pTORGm43GWR
Plasmid#112905PurposeMultisite (attR3/attR4) Gateway vector for expression in oomycetes. Confers resistance to gentamicin. Contains additional cassette for constitutive expression of tdTomato.DepositorTypeEmpty backboneUseOomycete expressionTagsExpressionMutationPromoterAvailable sinceJuly 8, 2019AvailabilityAcademic Institutions and Nonprofits only