Purpose
A CRISPR activation device with the necessary genes (dxcas9 controlled by Ptet, pspFΔHTH::λN22plus controlled by promoter PrhaB), and the reporter part (PpspA-LEA3B3 with sfgfp::ASV).
Inserts
dxcas9 (3.7)
tetR
rhaS
pspFΔHTH::λN22plus
sfgfp
Use
Synthetic Biology
Tags
ASV tag and pspFΔHTH::λN22plus
Expression
Bacterial
Mutation
Mutations D10A and H840A on WT cas9 for dCas9; Sy…
Promoter
Pcon, PpspA-LEA3B3, PrhaB, and Ptet
Available since
Sept. 23, 2019
Availability
Academic Institutions and Nonprofits only
