Addgene

We narrowed to 2,242 results for: PRK

Showing: 341 - 360 of 2242 results

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pWPI-FLAG-hPRKCI

Plasmid

#35387

Depositor

Luke McCaffrey

Insert

Protein Kinase C iota (PRKCI Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

June 26, 2014

Availability

Academic Institutions and Nonprofits only
pX462-hPRKAA2-gRNA_A

Plasmid

#74376

Purpose

gRNA_A to knockout human AMPK alpha 2 using Cas9n.

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-FLAG NUFIP1

Plasmid

#110952

Purpose

Transient expression of FLAG NUFIP1

Depositor

David Sabatini

Article

Wyant et al Science. 2018 May 18;360(6390):751-758.

Insert

NUFIP1 (NUFIP1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-SOX2-WT

Plasmid

#194580

Purpose

Mammalian Expression of mEGFP- SOX2 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

SOX2 (SOX2 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pX462-hPRKAA2-gRNA_B

Plasmid

#74377

Purpose

gRNA_B to knockout human AMPK alpha 2 using Cas9n

Depositor

Reuben Shaw

Article

Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

Insert

human AMPK alpha 2 exon1 gRNA (PRKAA2 Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

U6

Available since

April 14, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-PRAS40 T246A

Plasmid

#15482

Depositor

Do-Hyung Kim

Article

Vander Haar et al Nat Cell Biol. 2007 Mar . 9(3):316-23.

Insert

PRAS40 (AKT1S1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Thr246 to Ala

Promoter

Available since

Aug. 21, 2007

Availability

Academic Institutions and Nonprofits only
USP19-Flag/pRK5

Plasmid

#36306

Depositor

Xiaolu Yang

Article

Mei et al J Biol Chem. 2011 Oct 14;286(41):35380-7

Insert

ubiquitin specific peptidase 19 (USP19 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 11, 2012

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-PHOX2B-WT

Plasmid

#194576

Purpose

Mammalian Expression of mEGFP-PHOX2B WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

PHOX2B (PHOX2B Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-HA-YFP-DEPTOR

Plasmid

#73390

Purpose

Expresses HA and YFP tagged DEPTOR

Depositor

Jie Chen, Taekjip Ha

Article

Jain et al Proc Natl Acad Sci U S A. 2014 Dec 16;11

Insert

DEP Domain Containing MTOR-Interacting Protein (DEPTOR Human)

Use

Tags

HA and YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 24, 2016

Availability

Academic Institutions and Nonprofits only
Cterminal HA Mios pRK5

Plasmid

#46329

Depositor

David Sabatini

Article

Bar-Peled et al Science. 2013 May 31;340(6136):1100-6. d

Insert

Mios (MIOS Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

July 15, 2013

Availability

Academic Institutions and Nonprofits only
pRK5myc RhoA N19

Plasmid

#15901

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Use

Tags

myc

Expression

Mammalian

Mutation

T19N

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pRK5-FLAG-Sestrin3

Plasmid

#72592

Purpose

overexpression, codon optimized for mammalian expression

Depositor

David Sabatini

Article

Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

Insert

SESN3 (SESN3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

codon optimized for mammalian expression, see dep…

Promoter

CMV

Available since

March 28, 2016

Availability

Academic Institutions and Nonprofits only
pRK5myc RhoA L63

Plasmid

#15900

Depositor

Alan Hall

Article

Nobes et al J Cell Biol. 1999 Mar 22. 144(6):1235-44

Insert

RhoA (RHOA Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Q63L

Promoter

Available since

Nov. 15, 2007

Availability

Academic Institutions and Nonprofits only
pUB6-CDC34-HA (pRKB261)

Plasmid

#99145

Purpose

Mammalian expression construct to express HA-tagged CDC34

Depositor

Robert Bradley

Article

Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

Insert

CDC34 (CDC34 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

pTight

Available since

Nov. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pRK5-mouse-DJ1-HA

Plasmid

#29396

Depositor

Mark Cookson

Article

Miller et al Neurosci Lett. 2005 Feb 21. 374(3):203-6

Insert

DJ1 (Park7 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 1, 2011

Availability

Academic Institutions and Nonprofits only
pRK5-mEGFP-HMGB3-WT

Plasmid

#194564

Purpose

Mammalian Expression of mEGFP-HMGB3 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

HMGB3 (HMGB3 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5-mEGFP-FOXF1-WT

Plasmid

#194568

Purpose

Mammalian Expression of mEGFP-FOXF1 WT Fusion Protein

Depositor

Denes Hnisz

Article

Mensah et al Nature. 2023 Feb;614(7948):564-571. doi:

Insert

FOXF1 (FOXF1 Human)

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 mFz8CRD-IgG

Plasmid

#16689

Depositor

Xi He

Article

Semënov et al Curr Biol. 2001 Jun 26. 11(12):951-61.

Insert

Frizzled-8 (Fzd8 Mouse)

Use

Tags

IgG

Expression

Mammalian

Mutation

CRD domain of mFz8

Promoter

Available since

May 16, 2008

Availability

Academic Institutions and Nonprofits only
pRK7 IKK2/IKKb

Plasmid

#26203

Depositor

Tom Maniatis

Article

Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

Insert

I-kappa-B kinase beta (IKBKB Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
pRK5 FLAG DEPTOR (13×S/T->A)

Plasmid

#21702

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 13. ():.

Insert

DEPTOR 13xA (DEPTOR Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Nonphosphorylatable mutant. 13xA. Also has N204S …

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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