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p3xFlag-CMV14-hISD11

Plasmid

#31897

Depositor

Gino Cortopassi

Article

Shan et al Hum Mol Genet. 2007 Apr 15;16(8):929-41.

Insert

ISD11 (LYRM4 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only
mEos2-FilaminA-C-14

Plasmid

#57378

Purpose

Localization: Actin/Focal Adhesions, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

FilaminA (FLNA Human)

Use

Tags

mEos2

Expression

Mammalian

Mutation

D2626H

Promoter

CMV

Available since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits
TAL2271

Plasmid

#36708

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

GLI1-TALEN_Right (GLI1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
TAL2270

Plasmid

#36707

Depositor

Keith Joung

Article

Reyon et al Nat Biotechnol. 2012 Apr 8. doi: 10.1038

Insert

GLI1-TALEN_Left (GLI1 Human)

Use

Mammalian, t7 for in vitro mrna synthesis

Tags

3X Flag and WT FOKI

Expression

Mutation

Promoter

CMV

Available since

June 26, 2012

Availability

Academic Institutions and Nonprofits only
pJR015

Plasmid

#198812

Purpose

Epidermal growth factor

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::Lin-3c-SL2-GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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pJR026

Plasmid

#198824

Purpose

Lysosomal associated membrane protein

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::lmp1-venus::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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pHW450

Plasmid

#198831

Purpose

Voltage-sensitive fluorescent protein.

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::VSFP-Butterfly-1-2::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pHW394 (15xUAS::GFP::let-858 3'UTR)

Plasmid

#85584

Purpose

cGAL effector (15xUAS::GFP::let-858 3'UTR) construct for C. elegans

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

15xUAS-delta pes-10-GFP

Use

Tags

Expression

Worm

Mutation

GFP(S65C) with three introns for expression in C.…

Promoter

15xUAS-delta pes-10-GFP

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1-sh-hSOX9-1

Plasmid

#40644

Depositor

Bob Weinberg

Article

Guo et al Cell. 2012 Mar 2;148(5):1015-28.

Insert

sh-hSOX9-1 (SOX9 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
NSUN6

Plasmid

#188060

Purpose

Bacterial expression plasmid for human NSUN6; for activity testing

Depositor

Tanja Schirmeister

Article

Schwickert et al J Med Chem. 2022 Jul 18. doi: 10.1021/ac

Insert

NSUN6 (NSUN6 Human)

Use

Tags

His-tag

Expression

Bacterial

Mutation

Promoter

T7 promoter

Available since

Aug. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pHW393 (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR)

Plasmid

#85583

Purpose

cGAL driver (Prab-3::GAL4-SK(DBD)::VP64::let-858 3'UTR) construct for C. elegans using the S. kudriavzevii (SK) GAL4 DNA-binding domain (DBD)

Depositor

Paul Sternberg

Article

Wang et al Nat Methods. 2016 Dec 19. doi: 10.1038/n

Insert

Prab-3-GAL4-SK(DBD)-VP64

Use

Tags

Expression

Worm

Mutation

Promoter

Prab-3-GAL4-SK(DBD)-VP64

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pLUT7 HA-GLI1

Plasmid

#62970

Purpose

Expresses HA-tagged Gli1

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

GLI1 (GLI1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBABEPuro-HA-GLI1

Plasmid

#62967

Purpose

Expresses HA-tagged Gli1

Depositor

Michael Ruppert

Article

Deng et al J Cell Sci. 2015 Jan 27. pii: jcs.162164

Insert

GLI1 (GLI1 Human)

Use

Retroviral

Tags

HA

Expression

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only
pJR019

Plasmid

#198834

Purpose

Inactivation of G protein subunits through Pertussis toxin (PTX)

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

15xUAS::PTX-SL2-GFP::let-858 3'UTR

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3-myc-FLNa S2152A

Plasmid

#8983

Depositor

John Blenis

Article

Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

Insert

Filamin A S2152A (FLNA Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Ser2152Ala

Promoter

Available since

Oct. 11, 2005

Availability

Academic Institutions and Nonprofits only

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pNH11 (pmyo-2::Arch(D95N)::2xMycTag)

Plasmid

#130275

Purpose

Expresses the voltage sensor Arch(D95N) in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

Arch(D95N)

Use

Tags

2x Myc-tag

Expression

Worm

Mutation

Promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pET21c(+)-mtPheRS

Plasmid

#31080

Depositor

Linda Spremulli

Article

Bullard et al J Mol Biol. 1999 May 14. 288(4):567-77.

Insert

Mature form human mitochondrial phenylalanine-tRNA synthetase (FARS2 Human)

Use

Tags

His

Expression

Bacterial

Mutation

Includes coding sequence for the mature form. Nt …

Promoter

T7

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pNH13 (pmyo-2::QuasAr::mOrange)

Plasmid

#130273

Purpose

Expresses the eFRET-based voltage sensor QuasAr::mOrange in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

QuasAr::mOrange

Use

Tags

mOrange

Expression

Worm

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pNH12 (pmyo-2::MacQ::mCitrine)

Plasmid

#130274

Purpose

Expresses the eFRET-based voltage sensor MacQ::mCitrine in the pharynx of C. elegans.

Depositor

Alexander Gottschalk

Article

Azimi Hashemi et al Proc Natl Acad Sci U S A. 2019 Aug 1. pi

Insert

MacQ::mCitrine

Use

Tags

mCitrine

Expression

Worm

Mutation

Promoter

Available since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-GLI(-)TAD

Plasmid

#37113

Depositor

David Walterhouse

Article

Yoon et al J Biol Chem. 1998 Feb 6;273(6):3496-501.

Insert

GLI1 (GLI1 Human)

Use

Tags

Expression

Mammalian

Mutation

deleted 1,409 bp AccI fragment from 3' end o…

Promoter

CMV

Available since

Aug. 20, 2013

Availability

Academic Institutions and Nonprofits only