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Plasmid#228689PurposeN terminal dual protein A affinity tag with TEV cleavage siteDepositorInsert2xProtein A-TEV
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM.010
Plasmid#228696PurposeC terminal Strep affinity tag with TEV cleavage siteDepositorInsertTEV-Strep
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM.009
Plasmid#228695PurposeN terminal Strep affinity tag with TEV cleavage siteDepositorInsertStrep-TEV
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM.002
Plasmid#228688PurposeC terminal MBP affinity tag with PreScission Protease cleavage siteDepositorInsertPrs-MBP
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pM.001
Plasmid#228687PurposeN terminal MBP affinity tag with PreScission Protease cleavage siteDepositorInsertMBP-Prs
UseTagsExpressionInsectMutationWTPromoterAvailable sinceDec. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCENTR_DMC1p_amiRNA
Plasmid#225985PurposeTo drive any artificial microRNA under the regulatory control of AtDMC1 promoter and it will make a gateway entry cloneDepositorTypeEmpty backboneUseRNAiTagsExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pGLU_67
Plasmid#225770PurposeThis is the native promoter from the aph(3')-I kanamycin resistance cassette.DepositorInsertPaph(3')-I_[abr]
UseSynthetic BiologyTagsExpressionMutationN/APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
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p426 3-F GW
Plasmid#58556PurposepRS426 carrying Gateway cassette for three insertsDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceNov. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRedCmOcr
Plasmid#176226PurposeA helper plasmid for expression of the artificial T7ocr-gam-bet-exo operon. The plasmid carries the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage.DepositorInsertLambda Red-T7ocr operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPrhaB and PLAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCF1148
Plasmid#222330PurposeFluorescent reporter plasmid for VeillonellaDepositorInsertbs2 gene with Veillonella parvula SKV38 mdh promoter
UseE. coli-veillonella shuttle plasmidTagsExpressionBacterialMutationPromoterVeillonella parvula SKV38 mdh promoterAvailable sinceOct. 31, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBM019
Plasmid#128179PurposeConstruct for generating stable Cas9-expressing Toxoplasma gondiiDepositorInsertssgRNA#1
SpCas9
Chloramphenicol acetyltransferase
UseCRISPR; Toxoplasma gondii expressionTags3X FLAG and Nuclear Localization SignalExpressionMutationPromoterTUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…Available sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCas9-CAT
Plasmid#80323PurposeEncodes Cas9 and chloramphenicol acetyltransferase (CAT)DepositorInsertsCas9
Chloramphenicol acetyltransferase
UseCRISPRTags3X FLAG and NLSExpressionMutationPromoterTgTUB1Available sinceSept. 12, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-OsMIR390-B/c
Plasmid#61468PurposeGateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.DepositorInsertOsMIR390-B/c
UseGateway entry vectorTagsExpressionMutationO. sativa MIR390 precursor sequence including a c…PromoterAvailable sinceMarch 2, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-Nanoluc
Plasmid#87067PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseTagsNanoluc luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pENTR_AtU6p_BsaI_ccdb_gRNA
Plasmid#225986PurposeTo make Gateway entry clone of CRISPR guide RNA under AtU6pDepositorTypeEmpty backboneUseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceOct. 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-Nanoluc-ccdB
Plasmid#87070PurposeMammalian Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferaseDepositorTypeEmpty backboneUseTagsNanoluc luciferaseExpressionMammalianMutationPromoterAvailable sinceAug. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-Renilla
Plasmid#87065PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Renilla luciferaseDepositorTypeEmpty backboneUseTagsRenilla luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Firefly-ccdB
Plasmid#87069PurposeMammalian Gateway-compatible expression vector backbone with an N-terminal Firefly luciferaseDepositorTypeEmpty backboneUseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ccdB-Firefly
Plasmid#87066PurposeMammalian Gateway-compatible expression vector backbone with a C-terminal Firefly luciferaseDepositorTypeEmpty backboneUseTagsFirefly luciferaseExpressionMammalianMutationPromoterAvailable sinceAug. 16, 2017AvailabilityAcademic Institutions and Nonprofits only