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pCIP-Flag-TfebAA

Plasmid

#79014

Purpose

For mammalian expression of a Flag-tagged, constitutively active version (S142A, S211A) of Tfeb.

Depositor

Reuben Shaw

Article

Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

Insert

Tfeb (Tfeb Mouse)

Use

Tags

Flag

Expression

Mammalian

Mutation

S142A, S211A

Promoter

pCAGGs

Available since

July 15, 2016

Availability

Academic Institutions and Nonprofits only

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SpdCas9 KRAB

Plasmid

#188499

Purpose

Expresses FLAG tagged SpdCas9 KRAB

Depositor

Isaac Hilton

Article

Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

Insert

dCas9

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

D10A/H840A

Promoter

EF1a

Available since

Nov. 9, 2022

Availability

Academic Institutions and Nonprofits only

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WT GLUT1-FLAG

Plasmid

#200094

Purpose

WT human GLUT1 with a C-terminal 3xFLAG tag in pQCXIP vector

Depositor

Jason MacGurn

Article

Qualls-Histed et al iScience. 2023 Feb 6;26(3):106150. doi:

Insert

GLUT1 (SLC2A1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-Cdc42-2G

Plasmid

#68813

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Lentivirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Lentiviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pBABEpuro HA Brca1

Plasmid

#14999

Depositor

Stephen Elledge

Article

Cortez et al Science. 1999 Nov 5. 286(5442):1162-6.

Insert

Brca1 (BRCA1 Human)

Use

Retroviral

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

June 19, 2007

Availability

Academic Institutions and Nonprofits only

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pLVX-RT003-GPM

Plasmid

#163383

Purpose

To express sGFP module in the G-baToN system

Depositor

Monte Winslow

Article

Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

Insert

GFP-PDGFRtm-2A-mCherry (GPM)

Use

Lentiviral

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

EF1a

Available since

Jan. 11, 2021

Availability

Academic Institutions and Nonprofits only

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pMXs-puro-RFP-ATG9A

Plasmid

#60609

Purpose

Expresses RFP-hATG9A in mammalian cells

Depositor

Noboru Mizushima

Article

Koyama-Honda et al Autophagy. 2013 Oct;9(10):1491-9. doi: 1

Insert

Homo sapiens autophagy related 9A (PPARG Human)

Use

Retroviral

Tags

RFP

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 20, 2015

Availability

Academic Institutions and Nonprofits only

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mycBioID2-pBABE-puro

Plasmid

#80900

Purpose

To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); a small MCS is present downstream of myc tag and BioID2 tag

Depositor

Kyle Roux

Article

Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Retroviral

Tags

humanized A. aeolicus BioID2 with R40G and myc

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-puro-TagBFP-β-ACTIN-3′UTR-24xMS2

Plasmid

#205161

Purpose

express β-ACTIN-3′UTR-24xMS2

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

β-ACTIN-3′UTR-24xMS2

Use

Tags

tagBFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Ki67-mNeonGreen-IRESpuro2

Plasmid

#183737

Purpose

Expresses Ki67-mNeonGreen

Depositor

Daniel Gerlich

Article

Cuylen et al Nature. 2016 Jun 29;535(7611):308-12. do

Insert

MKI67 (MKI67 Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 27, 2022

Availability

Academic Institutions and Nonprofits only

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human STIM1 YFP

Plasmid

#19754

Depositor

Anjana Rao

Article

Prakriya et al Nature. 2006 Sep 14. 443(7108):230-3.

Insert

STIM1 (STIM1 Human)

Use

Retroviral

Tags

YFP

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only

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pLVX_DSB_Spectrum-V2

Plasmid

#192475

Purpose

Lentiviral DSB Spectrum V2 Reporter System

Depositor

Michael Yaffe

Article

van de Kooij et al Nat Commun. 2022 Sep 8;13(1):5295. doi:

Insert

BFP1.2, incompleteGFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pLPC-Flag-PML-IV

Plasmid

#62804

Purpose

expresses the isoform IV of PML (promyelocytic leukemia protein)

Depositor

Gerardo Ferbeyre

Article

Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

Insert

PML-IV (PML Human)

Use

Retroviral

Tags

3X-FLAG

Expression

Mammalian

Mutation

Promoter

Available since

April 2, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA-puro-TagBFP-β-ACTIN-3′UTR-8xMS2

Plasmid

#205160

Purpose

express β-ACTIN-3′UTR-8xMS2

Depositor

Weirui Ma

Article

Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

Insert

β-ACTIN-3′UTR-24xMS2

Use

Tags

tagBFP

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-FoxO1-Clover

Plasmid

#67759

Purpose

Fluorescent fusion protein for tracking Akt signaling

Depositor

Peter Rotwein

Article

Gross et al J Cell Sci. 2015 Jun 3. pii: jcs.168773.

Insert

Fox01 (Foxo1 Mouse)

Use

Lentiviral

Tags

Clover

Expression

Mutation

Changed Serine 209 to Alanine, changed Histidine …

Promoter

EF1a

Available since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only

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pPUR-hU6-sgRNA-Sirius-8XMS2

Plasmid

#121942

Purpose

CRISPR-Sirius plasmid

Depositor

Thoru Pederson

Article

Ma et al Nat Methods. 2018 Nov;15(11):928-931. do

Insert

sgRNA-Sirius-8XMS2

Use

CRISPR and Lentiviral

Tags

no

Expression

Mammalian

Mutation

Promoter

human U6 promoter

Available since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pgRNA-modified

Plasmid

#84477

Purpose

guide RNA expression lentiviral construct

Depositor

Rudolf Jaenisch

Article

Liu et al Cell. 2016 Sep 22;167(1):233-247.e17. do

Insert

guide RNA

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

mU6

Available since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pMH-Flag-HA

Plasmid

#101766

Purpose

Gateway-compatible destination vector for mammalian expression of N-terminal HA & Flag-tag fusion protein

Depositor

Michael Huen

Article

An et al Proc Natl Acad Sci U S A. 2017 Apr 4;114

Type

Empty backbone

Use

Tags

Flag-HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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p6872 pHAGE-N-V5-MAML1-FL

Plasmid

#37048

Depositor

Peter Howley

Article

Tan et al Proc Natl Acad Sci U S A. 2012 Apr 30.

Insert

pHAGE-N-V5-MAML1-FL (MYLIP Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMVt

Available since

July 20, 2012

Availability

Academic Institutions and Nonprofits only

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pPP441

Plasmid

#158801

Purpose

Plasmid containing Homo sapiens codon optimized CasΦ-2 and a SapI-GG stuffer spacer for editing in human cells.

Depositor

Jennifer Doudna

Article

Pausch et al Science. 2020 Jul 17;369(6501):333-337.

Insert

CasPhi-2

Use

Tags

2xSV40NLS-2xFLAG

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2020

Availability

Academic Institutions and Nonprofits only

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