Addgene

We narrowed to 6,915 results for: chloramphenicol

Showing: 3561 - 3580 of 6915 results

Results per page:

Sort results by:

pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

PcUBI4-2

Available since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pRK793

Plasmid

#8827

Depositor

David Waugh

Article

Kapust et al Protein Eng. 2001 Dec . 14(12):993-1000.

Insert

TEV protease, S219V mutant

Use

Tags

His, MBP (with TEV), and polyarginine

Expression

Bacterial

Mutation

S219V mutation (improves stability)

Promoter

Available since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

Enlarge
pDae079 (eMutaT7[transition])

Plasmid

#187622

Purpose

Expresses eMutaT7[TadA-8e], eMutaT7[PmCDA1] and UGI for mutagenizing genes downstream from a T7 promoter in e.coli

Depositor

Seokhee Kim

Article

Seo et al Nucleic Acids Res. 2023 Apr 18:gkad266.

Inserts

TadA-8e-T7RNAP
PmCDA1-T7RNAP

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pTECH-chPylRS(IPYE)

Plasmid

#99222

Purpose

Expression plasmid encoding chPylRS(IPYE) and pylT

Depositor

Dieter Söll

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chPyl(IPYE)RS; PproK pylT

Use

Tags

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pEVOL-pylT-AznLRS

Plasmid

#172482

Purpose

For E.Coli expression, 306L309L348I384F411W

Depositor

Wenshe Liu

Article

Wang et al Angew Chem Int Ed Engl. 2017 Feb 1;56(6)

Insert

AznLRS and pylTcua

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD

Available since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pNP1950

Plasmid

#222729

Purpose

J23119-BsaI_bRNA Golden Gate Landing Pad-HDV; T7-IS621 Recombinase

Depositor

Patrick Hsu

Article

Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG030 (TerY-P)

Plasmid

#157908

Purpose

Expresses the TerY-P defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

TerY-P triad (vWA + kinase + phosphatase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG029 (mzaABCDE)

Plasmid

#157907

Purpose

Expresses the mzaABCDE defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

mzaABCDE (5-gene system containing a MutL homolog)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 16, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG003 (Druantia type I)

Plasmid

#157881

Purpose

Expresses the Druantia type I defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

Druantia type I

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG034 (restriction-like)

Plasmid

#157912

Purpose

Expresses a restriction-like defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

4-gene restriction-like system

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG026 (tmn)

Plasmid

#157904

Purpose

Expresses the tmn defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

tmn (transmembrane ATPase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pLG031 (upx)

Plasmid

#157909

Purpose

Expresses the upx defense system

Depositor

Feng Zhang

Article

Gao et al Science. 2020 Aug 28;369(6507):1077-1084

Insert

upx (putative PD-(D/E)xK family phosphodiesterase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
3TERM_bba_b0015_rev_chlo

Plasmid

#163931

Purpose

L0 part - terminator

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

from GenBank: KT364744.3, prokaryotic double terminator BBa_B0015

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pcDNA3.1-3xFLAG-V5-ccdB

Plasmid

#87064

Purpose

Mammalian Gateway-compatible expression vector backbone with an N-terminal 3xFLAG-V5 tag

Depositor

Mikko Taipale, Susan Lindquist

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1-ccdB-3xFLAG-V5

Plasmid

#87063

Purpose

Mammalian Gateway-compatible expression vector backbone with a C-terminal 3xFLAG-V5 tag

Depositor

Mikko Taipale, Susan Lindquist

Article

Taipale et al Cell. 2012 Aug 31;150(5):987-1001. doi:

Type

Empty backbone

Use

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Promoter

Available since

March 6, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAIDA1

Plasmid

#79180

Purpose

A construct with the AIDA surface expression system for display of a passenger protein flanked by His and Myc tags

Depositor

Gen Larsson

Article

Jarmander et al Microb Cell Fact. 2012 Sep 3;11:118. doi

Insert

Adhesin Involved in Diffuse Adherence

Use

Tags

His and Myc

Expression

Bacterial

Mutation

Promoter

lacUV5

Available since

Jan. 27, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pACYC-GroEL/ES-TF

Plasmid

#83923

Purpose

Co-expresses cytoplasmic copies of the Gro EL/ES and Trigger Factor protein folding chaperones.

Depositor

Karl Griswold

Article

Lamppa et al J Biotechnol. 2013 Mar 10;164(1):1-8. do

Inserts

Gro EL/ES
Trigger Factor

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Nov. 28, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CA

Plasmid

#20960

Purpose

piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Mar 1. ():.

Insert

Gateway Destination Vector

Use

piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

Enlarge
IBMc090

Plasmid

#161937

Purpose

Carries engineered transposon for IBM

Depositor

Baojun Wang

Article

Ho et al Nat Commun. 2021 Apr 13;12(1):2200. doi:

Insert

pTHSSd_1V-WTR1R2-BbsI(G)-kanR-lacZalpha-SapI-WTR1R2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 14, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti_C_Myc_DDK_IRES_Puro_PRKRA

Plasmid

#106113

Purpose

Lentiviral expression of PRKRA

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2018 Feb 15;69(4):622-635.e6.

Insert

PRKRA (PRKRA Human)

Use

Lentiviral

Tags

DDK (FLAG) and Myc

Expression

Mutation

Promoter

Available since

Feb. 13, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge