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His_CL7_2NLS_iCas12a-4NLS

Plasmid

#222971

Purpose

Bacterial expression of NLS-rich iCas12a construct

Depositor

Jennifer Doudna

Article

Chen et al Nat Commun. 2024 Feb 26;15(1):1727. doi:

Insert

His_CL7_2NLS_iCas12a-4NLS

Use

CRISPR

Tags

CL7 and His

Expression

Bacterial

Mutation

Promoter

tac

Available since

July 26, 2024

Availability

Academic Institutions and Nonprofits only

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pCM4.4

Plasmid

#217648

Purpose

an ermE*p-cas9-driven expression, as an alternative plasmid

Depositor

Huimin Zhao

Article

Ye et al J Ind Microbiol Biotechnol. 2020 May;47(

Insert

Codon Optimized SpCas9

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

May 29, 2024

Availability

Academic Institutions and Nonprofits only

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pBTK1061

Plasmid

#209550

Purpose

BTK assembled plasmid for integrating a KanR+E2-Crimson cassette into the S. alvi genome in place of the clpA gene

Depositor

Jeffrey Barrick

Article

Lariviere et al bioRxiv 2023.09.19.558440

Insert

E2-Crimson cassette flanked with homology arms for recombination into the S. alvi wkB2 genome to replace clpA (SALWKB2_RS04465)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pAXY0019

Plasmid

#205992

Purpose

pNpuDnaE(C)_HygR(53-341)_3HA

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

NpuDnaE(C)-HygR

Use

Tags

3xHA

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAXY0018

Plasmid

#205587

Purpose

p3flag_HygR(1-52)-NpuDnaE(N)

Depositor

Xiaohan Yang

Article

Yuan et al Commun Biol. 2023 May 26;6(1):567. doi:

Insert

HygR-NpuDnaE(N)_homo-codon-OP

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV IE94

Available since

Aug. 29, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_2 [pBBR1 ORI]

Plasmid

#198919

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_22 [pSG5 ORI]

Plasmid

#198935

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pSG5 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_27 [Tfd gene VIII]

Plasmid

#198940

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

Tfd gene VIII

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_5 [pAL5000 ORI]

Plasmid

#198922

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pAL5000 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_24 [pBBR1-UP ORI]

Plasmid

#198937

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBBR1-UP ORI

Use

Synthetic Biology

Tags

Expression

Mutation

S100L (C299T)

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_37 [tetA pSC101]

Plasmid

#198950

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

tetA pSC101

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_3 [RSF1010 ORI]

Plasmid

#198920

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_36 [PtetA pSC101]

Plasmid

#198949

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

PtetA pSC101

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pUCSh2-2300

Plasmid

#170984

Purpose

Encodes sgRNA target 2300 and Kanamycin resistance transposon.

Depositor

Christopher Reisch

Article

Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

Insert

Sh-CAST sgRNA 2300

Use

CRISPR and Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

J23119

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_144

Plasmid

#199091

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_141

Plasmid

#199088

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 6, 2023

Availability

Academic Institutions and Nonprofits only

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pGL2_142

Plasmid

#199089

Purpose

Even ORI-marker screen destination vector with sfYFP dropout for barcoded-ORI insertion

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL0_115 [pBC1 ORI]

Plasmid

#199015

Purpose

Level 0 part

Depositor

Cultivarium Tools, Charlie Gilbert, Nili Ostrov

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

pBC1 ORI

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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CMVTO-gfp-Kir2.1-HA-tevs-KKYL

Plasmid

#179706

Purpose

TEVP-inducible GFP-conjugated Kir2.1 RELEASE with KKXX motif

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

Kir2.1

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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CMVTO-SEAP-26Sfur-3TM-hcvs-KKMP

Plasmid

#179635

Purpose

HCVP-inducible tri-transmembrane SEAP RELEASE construct

Depositor

Xiaojing Gao

Article

Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

Insert

SEAP RELEASE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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