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pJL017

Plasmid

#53532

Purpose

This plasmid encodes both the etpB and etpA two partner secretion genes on an arabinose-inducible plasmid with myc and 6His tags in-frame with the etpA gene

Depositor

James Fleckenstein

Article

Fleckenstein et al Nat Protoc. 2009;4(7):1083-92. doi: 10.1

Insert

etpBA

Use

Tags

6His and myc

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

June 10, 2014

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spa)_ctrA

Plasmid

#133342

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_gcrA1

Plasmid

#133340

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets gcrA gene's promoter on the non-template strand in Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pEG BacMam N term myc eGFP 3C

Plasmid

#160682

Purpose

Vector optimized for use in screening assays, as well as for efficient production of baculovirus and robust expression of the target protein

Depositor

Eric Gouaux

Article

Goehring et al Nat Protoc. 2014 Nov;9(11):2574-85. doi:

Type

Empty backbone

Use

Tags

myc eGFP 3C

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spy)_ctrA

Plasmid

#133334

Purpose

for constitutive expression of a single guide RNA from Streptococcus pyogenes with a seed region that targets ctrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_ctrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_24_positive_Control

Plasmid

#130570

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

FoxD3_cranial_neural_crest_enhancer_NC1

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pAGM4723-Del

Plasmid

#112207

Purpose

Level 2 cloning vector, for golden gate cloning for diatoms. All the Agrobacterium T-DNA delivery were deleted.

Depositor

Thomas Mock

Article

Graff van Creveld et al Elife. 2023 Jun 6;12:e84392. doi: 10.755

Type

Empty backbone

Use

Golden gate assembly

Tags

Expression

Mutation

Promoter

Available since

July 31, 2018

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_cpaA

Plasmid

#133344

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets cpaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_cpaA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pICH47742:PtFCP:Cas9YFP

Plasmid

#104894

Purpose

Cas9, with FCP promoter/terminator, Golden Gate Assembly, for Phaeodactylum transformation

Depositor

Thomas Mock

Article

Graff van Creveld et al Front Microbiol. 2021 Sep 8;12:688199. d

Insert

Cas9

Use

CRISPR; ; golden gate assembly

Tags

YFP

Expression

Mutation

Promoter

Available since

July 11, 2018

Availability

Academic Institutions and Nonprofits only

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pUASt-ssBiP-ESCargo*

Plasmid

#140165

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Drosophila melanogaster

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssBip-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Insect

Mutation

Promoter

hs70

Available since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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psecBFP2

Plasmid

#62230

Purpose

encodes an inert blue fluorescent protein for use in oxidizing cellular environments including the periplasm and mammalian secretory pathway.

Depositor

Erik Snapp

Article

Costantini et al Biochem Biophys Res Commun. 2013 Jan 18;

Insert

secBFP2

Use

Tags

secbfp2

Expression

Mammalian

Mutation

Promoter

cmv

Available since

July 29, 2015

Availability

Academic Institutions and Nonprofits only
pCVD447

Plasmid

#78569

Purpose

E. coli E2348/69 LEE region (Type 3 Secretion System) cloned into pBluescriptKS.

Depositor

James Kaper

Article

Jarvis et al Proc Natl Acad Sci U S A. 1995 Aug 15;92

Insert

lee operon

Use

Cloning vector

Tags

Expression

Mutation

Promoter

Available since

June 8, 2016

Availability

Academic Institutions and Nonprofits only
pCambia3301-AtU6-MAR1

Plasmid

#210757

Purpose

Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

Depositor

Daisuke Miki

Article

Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

Insert

AtU6-26 pro::MAR1 sgRNA

Use

CRISPR

Tags

Expression

Plant

Mutation

Promoter

AtU6-26

Available since

Nov. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D -IgK- TurboID- NCAN ELS-HA

Plasmid

#219809

Purpose

Secreted HA-tagged TurboID-NCAN-ELS domain for extracellular proximity labeling

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

GfaABC1D

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1-GfaABC1D -IgK-NCAN ELS-HA

Plasmid

#219811

Purpose

Secreted HA-tagged NCAN-ELS domain

Depositor

Cagla Eroglu

Article

Irala et al Neuron. 2024 Mar 27:S0896-6273(24)00161-

Insert

IgK (Igk Mouse)

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD063

Plasmid

#212916

Purpose

Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

mRuby2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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BPTI-21

Plasmid

#195058

Purpose

simplyfied amino acid sequence by alanine scanning

Depositor

Yutaka Kuroda, Peter Kim

Article

Kuroda et al J Mol Biol. 2000 May 5;298(3):493-501. d

Insert

bovine pancreatic trypsin inhibitor

Use

Tags

His tag and trpΔLE leader sequences

Expression

Bacterial

Mutation

21 amino acids are Alanine out of 58 amino acids

Promoter

T7 promotor

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140170

Purpose

Expresses a regulatable secretory cargo for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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pEarleyGate100-pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140169

Purpose

Expresses a regulatable secretory cargo (without an ER export signal) for Arabidopsis thaliana

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

pSpoA-DsRed-Express2-FKBP(LV)(C22V)

Use

Tags

Expression

Plant

Mutation

Promoter

CaMV 35S

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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ncvB-ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Plasmid

#140168

Purpose

Expresses a regulatable secretory cargo for Tetrahymena thermophila

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

ssGrl1-APVNTT-DsRed-Express2-FKBP(LV)(C22V)

Use

Other

Tags

Expression

Mutation

Promoter

MTT1

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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