Addgene

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pcDNA3-EGFP-AC8(1-106)

Plasmid

#181958

Purpose

Residues 1-106 of rat adenylyl cyclase 8 (AC8) tagged with EGFP.

Depositor

Jin Zhang

Article

Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1

Insert

EGFP-AC8(1-106) (Adcy8 Rat)

Use

Tags

6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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Str-STIM1-NN_neomycin

Plasmid

#65311

Purpose

Luminal ER hook only (RUSH system)

Depositor

Franck Perez

Article

Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

Insert

STIM1 mutated for binding motif to microtubule (STIM1 Human)

Use

Tags

streptavidin

Expression

Mammalian

Mutation

2 amino acids of STIM1 (IP from SxIP motif) were …

Promoter

CMV

Available since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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pKH3-TRIM28-C65A/C68A

Plasmid

#92199

Purpose

Mammalian overexpression vector for TRIM28; catalytically inactive (mutated on C65A and C68A)

Depositor

Huda Zoghbi

Article

Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

Insert

Tif1b (TRIM28 Human, Synthetic)

Use

Tags

HA

Expression

Mammalian

Mutation

Changed Cysteines 65 and 68 to Alanines.

Promoter

Available since

July 10, 2017

Availability

Academic Institutions and Nonprofits only

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pCK668

Plasmid

#192637

Purpose

Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

Inserts

Sp.pCas9-dCas9-NG
BBa_J23107-MCP-SoxS(R93A, S101A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

SoxS has R93A and S101A mutations and dCas9-NG ha…

Promoter

BBa_J23107 and Sp.pCas9

Available since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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ADD1

Plasmid

#155397

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ADD1 (ADD1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pDEST-mCherry-GJA1-20k-N1

Plasmid

#49861

Purpose

Encodes C-terminal fragment of human connexin 43 initiating at M213 with a V5 C-terminal fusion tag

Depositor

Robin Shaw

Article

Smyth et al Cell Rep. 2013 Nov 5. pii: S2211-1247(13

Insert

GJA1-20k (GJA1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

GJA1-20k corresponds to residues 213 to 382 to Co…

Promoter

CMV

Available since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pYFP-ErbB2ΔC990

Plasmid

#66946

Purpose

Mammalian expression of rat ErbB2 ΔC990 tagged with YFP

Depositor

Bo van_Deurs, Mads Lerdrup

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Use

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

Deletion of C990. (see depositor comment below on…

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
pYFP-ErbB2ΔC776

Plasmid

#66947

Purpose

Mammalian expression of rat ErbB2 ΔC776 tagged with YFP

Depositor

Bo van_Deurs, Mads Lerdrup

Article

Lerdrup et al Mol Biol Cell. 2007 Sep;18(9):3656-66. E

Insert

ErbB2 (Erbb2 Rat)

Use

Tags

6xHis, V5 Tag, and YFP

Expression

Mammalian

Mutation

Deletion of C776 (see depositor comment below on …

Promoter

CMV

Available since

Sept. 15, 2015

Availability

Academic Institutions and Nonprofits only
CBh-dCas9-Mll3SET

Plasmid

#108452

Purpose

Expression vector of CRISPR/dCas9 fused with SET domain of mouse MLL3 gene, driven by CBh promoter

Depositor

Bing Ren

Article

Yan et al Cell Res. 2018 Jan 9. pii: cr20181. doi:

Insert

dCas9, MLL3 (Kmt2c Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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MBP-CHC(1-1074)-His6

Plasmid

#100748

Purpose

Bacterial expression of MBP-tagged clathrin heavy chain large fragment including N-terminal domain, his tagged at C-terminus for purification.

Depositor

Stephen Royle

Article

Wood et al J Cell Biol. 2017 Sep 27. pii: jcb.20170

Insert

CLTC (CLTC Human)

Use

Tags

His and MBP

Expression

Bacterial

Mutation

Residues 1-1074

Promoter

Available since

Sept. 12, 2017

Availability

Academic Institutions and Nonprofits only

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LRRC59-His-bio

Plasmid

#52330

Purpose

Expresses full-length Leucine rich repeat containing 59 ectodomain in mammalian cells. N-terminal His tag, biotinylation sequence and rat Cd4d3+4 tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

LRRC59 (LRRC59 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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His6tev CBP HAT (1324-1700)

Plasmid

#99340

Purpose

bacterial expression of His tagged mouse CBP HAT domain (1324-1700)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP HAT domain (1324-1700) (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 6, 2017

Availability

Academic Institutions and Nonprofits only

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His6tev CBP BCHZ (1079-1751)

Plasmid

#99341

Purpose

Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

Depositor

Peter Wright

Article

Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

Insert

mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)

Use

Tags

His6tev

Expression

Bacterial

Mutation

Promoter

T7

Available since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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HPSE-bio-His

Plasmid

#53407

Purpose

Expresses full-length Heparanase precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

HPSE (HPSE Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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DDX5

Plasmid

#155523

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX5 (DDX5 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pS6-hα2M-H6

Plasmid

#128495

Purpose

Expresses human α2M (from G27 to S1468) in mammalian cells. With C-t H6x tag.

Depositor

F Xavier Gomis-Ruth

Article

Marino-Puertas et al Sci Rep. 2019 Jun 24;9(1):9186. doi: 10.

Insert

A2M (G27-S1468) (A2M Human)

Use

Tags

His tag (6x)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 15, 2019

Availability

Academic Institutions and Nonprofits only

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CCL5-bio-His

Plasmid

#52027

Purpose

Expresses full-length C-C motif chemokine 5 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

CCL5 (CCL5 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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ITGA2-bio-His

Plasmid

#51910

Purpose

Expresses full-length Integrin alpha-2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

Depositor

Gavin Wright

Article

Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

Insert

ITGA2 (ITGA2 Human)

Use

Tags

His tag, enzymatic biotinylation sequence, and ra…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 27, 2015

Availability

Academic Institutions and Nonprofits only

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pINDUCER-21-RUNX1-P2A-ERG

Plasmid

#97045

Purpose

Expresses RUNX1-P2A-ERG in mammalian cells. *Please see notes below on P2A cleaving efficiency.

Depositor

George Daley

Article

Sugimura et al Nature. 2017 May 17. doi: 10.1038/nature

Insert

Runt Related Transcription Factor 1, ETS-Related Gene

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet on

Available since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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CPSF1

Plasmid

#155475

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF1 (CPSF1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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