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Plasmid#215878PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-589(3p)-WT (MIR589 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-376a2(3p)-WT
Plasmid#215879PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-WT (MIR376A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-99a-WT
Plasmid#215880PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-99a-WT (MIR99A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-376a1(5p)-I-type
Plasmid#215882PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of 376a1(5p)-I-type (MIR376A1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-22(3p)-I-type
Plasmid#215886PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-22(3p)-I-type (MIR22 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-376a2(3p)-I-type
Plasmid#215887PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-376a2(3p)-I-type (MIR376A2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
psiCHECK-1-miR-99a-I-type
Plasmid#215888PurposeThis plasmid encodes luciferase which has microRNA binding sites in 3'UTR for detecting the microRNA silencing activity.DepositorInsertthree tandem repeats of seed-matched (SM) sequence of miR-99a-I-type (MIR99A Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2111-FKBP-GPA-20-11S
Plasmid#222882PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceSept. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS
Plasmid#216761PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2375-FKBP-GPA-20-11S
Plasmid#222897PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2376-FKBP-GPA-20-114
Plasmid#222896PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFKBP-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2374-FRB-GPA-20-11S
Plasmid#222895PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2373-FRB-GPA-20-114
Plasmid#222894PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceAug. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2110-FKBP-GPA-20-114
Plasmid#222881PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FKBP ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFKBP-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2108-FRB-GPA-20-114
Plasmid#222879PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.DepositorInsertFRB-GPA-20GS-NanoLuc114
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
L2109-FRB-GPA-20-11S
Plasmid#222880PurposeBiosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 11S.DepositorInsertFRB-GPA-20GS-NanoLuc11S
UseTagsMycExpressionMammalianMutationPromoterCMVAvailable sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
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PDE4B-Sensor
Plasmid#208929Purposein vitro JNK-Sensor for luminescent detection of JNK-activityDepositorInsertPDE4B docking sequence+Phospho site and Smbit (PDE4B Human)
UseTagsHISx6, MBP, and SmbitExpressionBacterialMutationPromoterT7Available sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pROF366
Plasmid#155332PurposeExpression of FLuc under the control of AtAP1 promoterDepositorInsertPAtAP1-FLuc-TSV40
UseLuciferase and Synthetic BiologyTagsExpressionPlantMutationPromoterAvailable sinceOct. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB399
Plasmid#203635PurposeModule for the expression of geneticin resistance, Nluc under PgpdA promoter and luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertFB367+FB396
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceOct. 18, 2023AvailabilityAcademic Institutions and Nonprofits only