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NDC80 F11.1 gRNA

Plasmid

#90786

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDC80

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDC80 (Guide Designation F11.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H1.1 gRNA

Plasmid

#90886

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H1.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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SGO1 H2.1 gRNA

Plasmid

#90887

Purpose

3rd generation lentiviral gRNA plasmid targeting human SGO1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

SGO1 (Guide Designation H2.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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ASF1B A4.2 gRNA

Plasmid

#90535

Purpose

3rd generation lentiviral gRNA plasmid targeting human ASF1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ASF1B (Guide Designation A4.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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ASF1B A3.2 gRNA

Plasmid

#90534

Purpose

3rd generation lentiviral gRNA plasmid targeting human ASF1B

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

ASF1B (Guide Designation A3.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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TUBE1 F6.4 gRNA

Plasmid

#90929

Purpose

3rd generation lentiviral gRNA plasmid targeting human TUBE1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TUBE1 (Guide Designation F6.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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BUB3 A10.1 gRNA

Plasmid

#90557

Purpose

3rd generation lentiviral gRNA plasmid targeting human BUB3

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

BUB3 (Guide Designation A10.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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NDEL1 F4.2 gRNA

Plasmid

#90792

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDEL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDEL1 (Guide Designation F4.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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NDEL1 F3.2 gRNA

Plasmid

#90790

Purpose

3rd generation lentiviral gRNA plasmid targeting human NDEL1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

NDEL1 (Guide Designation F3.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 24, 2017

Availability

Academic Institutions and Nonprofits only

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GTL-ir

Plasmid

#81102

Purpose

Gene Tagging vector LEU/iRFP - Plasmid for tandem infrared RFP (tdiRFP) labeling of endogenous proteins with a LEU selection marker, originating from pSIVl (ID:81090)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

tdiRFP

Use

Cre/Lox

Tags

tdiRFP

Expression

Yeast

Mutation

codon optimized second iRFP to avoid internal rec…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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GTT-y

Plasmid

#81110

Purpose

Gene Tagging vector TRP/YFP - Plasmid for monomeric Citrine (mCitrine) labeling of endogenous proteins with a TRP selection marker, originating from pSIVt (ID:81092)

Depositor

Serge Pelet

Article

Wosika et al Mol Genet Genomics. 2016 Dec;291(6):2231

Insert

mCitrine

Use

Cre/Lox

Tags

mCitrine

Expression

Yeast

Mutation

Mutated mCitrine for monomeric isoform (A206K L22…

Promoter

Available since

Nov. 16, 2016

Availability

Academic Institutions and Nonprofits only

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PPP2R5E C1.5 gRNA

Plasmid

#90855

Purpose

3rd generation lentiviral gRNA plasmid targeting human PPP2R5E

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

PPP2R5E (Guide Designation C1.5)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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TPX2 H2.2 gRNA

Plasmid

#90919

Purpose

3rd generation lentiviral gRNA plasmid targeting human TPX2

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

TPX2 (Guide Designation H2.2)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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CTPS1 E12.3 gRNA

Plasmid

#90645

Purpose

3rd generation lentiviral gRNA plasmid targeting human CTPS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CTPS1 (Guide Designation E12.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CTPS1 E11.3 gRNA

Plasmid

#90644

Purpose

3rd generation lentiviral gRNA plasmid targeting human CTPS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CTPS1 (Guide Designation E11.3)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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FBXO5 D4.4 gRNA

Plasmid

#90687

Purpose

3rd generation lentiviral gRNA plasmid targeting human FBXO5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FBXO5 (Guide Designation D4.4)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F1.1 gRNA

Plasmid

#90744

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F1.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F2.1 gRNA

Plasmid

#90745

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F2.1)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-NtermN1

Plasmid

#51445

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N1 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N1 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q

Promoter

CMV

Available since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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pBS EFEMP1

Plasmid

#19219

Depositor

Cliff Tabin

Article

Bandyopadhyay et al Dev Biol. 2008 Sep 1;321(1):162-74. doi:

Insert

EFEMP1 (EFEMP1 Chicken)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 19, 2008

Availability

Academic Institutions and Nonprofits only