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pLY017SB_pAAV-U6sg(BbsI)-EFS-Thy1.1-P2A-SB100X
Plasmid#192151PurposeT cell CRISPR AAV-SB vectorDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationNAPromoterAvailable sinceNov. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCherry-LOV2 V416L
Plasmid#81036Purposemammalian expression of LOV V416L, a mutant with super long relaxation timeDepositorInsertLOV V416L
UseTagsmCherryExpressionBacterial, Insect, and Mamm…MutationV416LPromoterAvailable sinceAug. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSEED
Plasmid#219658PurposePermits GoldenGate assembly of new SEED cassettes via Golden Gate assembly (BsaI)DepositorInsertEmpty SEED Cassette (3xP3-dsRed)
UseTagsExpressionMutationPromoterAvailable sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
stathmin-GFP
Plasmid#86782PurposeMammalian expression of stathmin-GFPDepositorInsertstathmin (STMN1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
VE-cad KI gRNA1
Plasmid#92310PurposeCRISPR-GFP-gRNA for cutting VEcadDepositorInsertVEcad KI gRNA1 (CDH5 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceJuly 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-Rho*-BERKY3
Plasmid#158220PurposeBRET biosensor for detection of Rho-GTP in mammalian expression vector pcDNA3.1(+)DepositorInsertLyn11-Nluc-ER/K linker-3xYFP-Rhotekin’s RBD (amino acids 7-89)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Ts-His6
Plasmid#224371PurposeExpress EF-Ts protein for his-tagged purificationDepositorInsertEF-Ts (tsf E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-G-His8
Plasmid#224369PurposeExpress EF-G protein for his-tagged purificationDepositorInsertEF-G (fusA E. coli)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-IF2
Plasmid#224367PurposeExpress IF2 protein for his-tagged purificationDepositorInsertIF2 (infB E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-RF3-His6
Plasmid#224373PurposeExpress RF3 protein for his-tagged purificationDepositorInsertRF3 (prfB E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-EF-Tu-His8
Plasmid#224370PurposeExpress EF-Tu protein for his-tagged purificationDepositorInsertEF-Tu (tufB E. coli)
UseTagsHis8ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-PheRSa-PheRSb
Plasmid#224364PurposeExpress PheRS protein for his-tagged purificationDepositorInsertPheRS (pheS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-AsnRS
Plasmid#224363PurposeExpress AsnRS protein for his-tagged purificationDepositorInsertAsnRS (asnS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-RRF-His6
Plasmid#224374PurposeExpress RRF protein for his-tagged purificationDepositorInsertRRF (frr E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-IF3
Plasmid#224368PurposeExpress IF3 protein for his-tagged purificationDepositorInsertIF3 (infC E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-IF1
Plasmid#224366PurposeExpress IF1 protein for his-tagged purificationDepositorInsertIF1 (infA E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-ThrRS
Plasmid#224365PurposeExpress ThrRS protein for his-tagged purificationDepositorInsertThrRS (thrS E. coli)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
PT7lacO-His6-CKM
Plasmid#224375PurposeExpress CKM protein for his-tagged purificationDepositorInsertCKM (LOC107051134 Chicken)
UseTagsHis6ExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2024AvailabilityAcademic Institutions and Nonprofits only
