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pLenti-LbCpf1-U6_FlipArray

Plasmid

#109349

Purpose

Lentiviral vector to express Cpf1 from EFS promoter, as well as a Cpf1 FlipArray from U6 promoter.

Depositor

Sidi Chen

Article

Chow et al Nat Commun. 2018 May 15;9(1):1903. doi:

Insert

U6-FlipArray

Use

CRISPR, Cre/Lox, Lentiviral, and Synthetic Biolog…

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 14, 2018

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPRv2-sgLmnb2-HepmCherry

Plasmid

#192830

Purpose

Expresses sgRNA targeting Lmnb2 and mCherry from hepatocyte-specific promoter

Depositor

Kristin Knouse

Article

Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

Insert

mCherry

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6 for sgRNA and hepatocyte-specific promoter (HS…

Available since

Nov. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pOPS0314

Plasmid

#115507

Purpose

Reporter plasmid suitable for experiments in environments where there is no antibiotic selection present. Constitutive promoter pNeo expressing celB

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

celB pOGG050, T-pharma pOGG003 assembled in pOGG026

Use

Tags

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Promoter

Constitutive promoter pNeo pOGG001

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pRPa-iSL(MCS1+2)

Plasmid

#69244

Purpose

Tet-inducible RRNA promoter driven plasmid for the generation of stem loop RNAi transcripts from a specific RRNA spacer locus in T. brucei.

Depositor

David Horn

Article

Alsford et al Mol Biochem Parasitol. 2008 Sep;161(1):7

Type

Empty backbone

Use

Expression in trypanosoma brucei

Tags

Expression

Mutation

Promoter

RRNA promoter

Available since

Oct. 28, 2015

Availability

Academic Institutions and Nonprofits only

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SPDK3959

Plasmid

#199246

Purpose

Modified TRV RNA2 with PEBV promoter and AtIleu-tRNA vector with AtPDS3 target for editing Arabidopsis PDS3 gene

Depositor

Savithramma Dinesh-Kumar

Article

Nagalakshmi et al Plant Physiol. 2022 Jun 27;189(3):1241-1

Insert

Modified TRV RNA2 with PEBV promoter, AtPDS3 target and AtIleu-tRNA mobile RNA sequence

Use

T-dna vector

Tags

Expression

Mutation

Promoter

PEBV

Available since

June 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBAC690-TEF

Plasmid

#160432

Purpose

Expresses eGFP under control of the yeast TEF2 promoter

Depositor

Gary Stormo

Article

Roy et al Commun Biol. 2020 Dec 11;3(1):761. doi:

Insert

S. cerevisiae TEF2 promoter

Use

Tags

Expression

Yeast

Mutation

Promoter

S. cerevisiae TEF2

Available since

Nov. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pBroad-T7RNAP-apraR

Plasmid

#199993

Purpose

T7 RNAP circuit variant T15 on ultra broad host range vector (RSF1010 and pAMB1 origins) apraA

Depositor

Farren Isaacs

Article

Patel et al Cell. 2022 Apr 28;185(9):1487-1505.e14.

Insert

T7RNAP Circuit

Use

Tags

Expression

Bacterial

Mutation

copFnull

Promoter

Available since

April 2, 2024

Availability

Academic Institutions and Nonprofits only

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mCitrine-USP19

Plasmid

#78593

Purpose

Express mCitrine-USP19 in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

USP19 (USP19 Human)

Use

Tags

mCitrine

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
4xHRE-MinTK-CRE

Plasmid

#141144

Purpose

lentiviral expression vector to generate a hypoxia fate-mapping system

Depositor

Daniele Gilkes

Article

Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

Insert

4xHRE-MinTK-CRE

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO-shTRAF2#1

Plasmid

#44127

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
pWB-TRAF2

Plasmid

#44114

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Retroviral

Tags

Flag

Expression

Mutation

Promoter

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only
pPTK003-2-pENO1

Plasmid

#84962

Purpose

Type 2, pENO1, Enolase 1 promoter

Depositor

Volker Sieber

Article

Obst et al ACS Synth Biol. 2017 Mar 15. doi: 10.102

Insert

Enolase 1 promoter

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

BsaI site removed (2350 g to c) and BsmBI site re…

Promoter

Non

Available since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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Flag-Rap1b V12

Plasmid

#118323

Purpose

Expression of bovine Rap1b

Depositor

Philip Stork

Article

Liu et al Mol Cell Biol. 2010 Aug;30(16):3956-69.

Insert

Rap1b V12 (RAP1B Bovine)

Use

Tags

Flag

Expression

Mammalian

Mutation

G12V

Promoter

CMV

Available since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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Nck

Plasmid

#186133

Purpose

For expression of recombinant Nck in bacteria cells.

Depositor

Michael Rosen

Article

Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

Insert

NCK1 (NCK1 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Amino acid residues 1-377, with mutations: C139S,…

Promoter

Lac

Available since

June 30, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-EF1adCas9VP64_T2A_MS2p65HSF1-IRESneopA

Plasmid

#112921

Purpose

Gateway entry vector carrying human EF1a promoter-driven dCas9VP64-T2A-MS2p65HSF1 with Neomycin resistant marker

Depositor

Kosuke Yusa

Article

Chong et al Genome Biol. 2018 Nov 26;19(1):205. doi:

Insert

EF1a promoter, dCas9VP64, MS2p65HSF1, IRESneo

Use

Gateway entry vector

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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PP255

Plasmid

#78934

Purpose

Promoter: scTEF1, minimal Promoter: GAP. Figure 2 and 4.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Insert

GFP

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pBTK151

Plasmid

#183127

Purpose

BTK Type 4 reverse promoter part for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al Science. 2020 Jan 31;367(6477):573-576.

Insert

Reverse CP25 promoter and forward rpoC terminator for dsRNA expression

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

May 10, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO-shTRAF2#2

Plasmid

#44128

Depositor

William Hahn

Article

Shen et al Mol Cell Biol. 2012 Dec;32(23):4756-68.

Insert

TRAF2 (TRAF2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

Available since

March 25, 2013

Availability

Academic Institutions and Nonprofits only
FRP1641_PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Plasmid

#58441

Purpose

Cassette to replace yeast promoters with an inducible promoter containing 3 lexA boxes

Depositor

Joerg Stelling

Article

Ottoz et al Nucleic Acids Res. 2014 Jul 17. pii: gku

Insert

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Use

Tags

Expression

Yeast

Mutation

Promoter

PTEF-HygMX-TTEF-insul-(lexA-box)3-PminCYC1

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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PP324

Plasmid

#78982

Purpose

246B. Promoter: GAP6, minimal Promoter: AOX1. Figure 5. Expresses rHGH and IFNa2b.

Depositor

Timothy Lu

Article

Perez-Pinera et al Nat Commun. 2016 Jul 29;7:12211. doi: 10

Inserts

rHGH
IFNa2b

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 23, 2016

Availability

Academic Institutions and Nonprofits only

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