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Showing: 8801 - 8850 of 148057 results

  5. MIP 247 CoMiP 4in1 with shRNA p53

    Plasmid
    #63726
    Purpose
    To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
    Depositor
    Mark Kay, Joseph Wu
    Article
    Diecke et al Sci Rep. 2015 Jan 28;5:8081. doi: 10.103
    Insert
    Mini-intronic-plasmid intron (POU5F1, KLF4, SOX2, MYC, TP53 Human)
    Use
    Tags
    IRES-dtomato
    Expression
    Mammalian
    Mutation
    codon-optimised
    Promoter
    SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
    Available since
    Aug. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    63726-plasmid-map-sequence-id-438433

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  11. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Article
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    170103-plasmid-map-sequence-id-332603

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  12. pHCL149

    Plasmid
    #134457
    Purpose
    CmR, Para-popZ-rbs-H3H4-msfGFPN-CTM. Replicative plasmid for the expression of bait. N-terminal GFP fusion.
    Depositor
    Thomas Bernhardt
    Article
    Lim et al Mol Microbiol. 2019 Sep 24. doi: 10.1111
    Insert
    Para-popZ-rbs-H3H4-msfGFPN-CTM.
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Para
    Available since
    Jan. 31, 2020
    Availability
    Academic Institutions and Nonprofits only
    134457-plasmid-map-sequence-id-270548

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  13. pX551-CMV-SpCas9

    Plasmid
    #107024
    Purpose
    AAV plasmid expressing Cas9 under control of CMV promoter, by replaceing mecp2 promoter in pX551 from Zhang lab
    Depositor
    Alex Hewitt
    Insert
    SpCas9
    Use
    AAV and CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    April 18, 2018
    Availability
    Academic Institutions and Nonprofits only
    107024-plasmid-map-sequence-id-208524

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  15. R777-E245 Hs.RASGRP2

    Plasmid
    #70529
    Purpose
    Gateway ORF clone of human RASGRP2 [NM_153819.1] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    RASGRP2 (RASGRP2 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70529-plasmid-map-sequence-id-125978

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  18. SUV420H2

    Plasmid
    #32864
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    SUV420H2 (KMT5C Human)
    Use
    Tags
    His tag (with TEV protease)
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 6, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  21. Luc-T20

    Plasmid
    #177941
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-22222 (T20) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Article
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Insert
    Luc2 (ORF1ab )
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    177941-plasmid-map-sequence-id-348207

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  22. TFORF1332

    Plasmid
    #143790
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    TFAP2E (TFAP2E Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    143790-plasmid-map-sequence-id-391571

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  26. pTF-lacZ

    Plasmid
    #153036
    Purpose
    For CRISPR-Cas12a genome editing in E. coli for the insertion of large DNA fragments at the lacZ locus. Contains a constitutively expressed CRISPR array targeting the lacZ gene . No cargo DNA.
    Depositor
    Nigel Scrutton
    Article
    Jervis et al Microb Biotechnol. 2021 Mar 12. doi: 10.
    Insert
    CRISPR array
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 7, 2021
    Availability
    Academic Institutions and Nonprofits only
    153036-plasmid-map-sequence-id-318836

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  27. pHLmMBP-3

    Plasmid
    #72345
    Purpose
    Mammalian expression of secreted N-terminally 8His-tagged mMBP-fused proteins with C-terminal 6His-tag/Strep-tag II/HA-tag
    Depositor
    Luca Jovine
    Article
    Bokhove et al J Struct Biol. 2016 Feb 3. 194:1-7. pii
    Type
    Empty backbone
    Use
    Tags
    6His-tag, 8His-tag, HA-tag, Strep-tag II, and mMB…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV enhancer + chicken beta-actin promoter
    Available since
    Feb. 17, 2016
    Availability
    Academic Institutions and Nonprofits only
    72345-plasmid-map-sequence-id-133752

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  28. pUCC001

    Plasmid
    #124451
    Purpose
    Multi-species CRISPR/Cas9 coexpression system, optimized for Golden Gate cloning of gRNA targets
    Depositor
    John Morrissey
    Article
    Rajkumar et al Front Bioeng Biotechnol. 2019 May 7;7:97
    Insert
    HH-BsaI
    Use
    Tags
    Expression
    Yeast
    Mutation
    gRNA expression cassette from pUDP002 modified to…
    Promoter
    TDH3 promoter from S.cerevisiae (already present …
    Available since
    Sept. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    124451-plasmid-map-sequence-id-257763

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  33. R777-E037 Hs.DUSP3

    Plasmid
    #70321
    Purpose
    Gateway ORF clone of human DUSP3 [NM_004090.3] with stop codon (for native or N-terminal fusions)
    Depositor
    Dominic Esposito
    Insert
    DUSP3 (DUSP3 Human)
    Use
    Gateway entry clone
    Tags
    Expression
    Mutation
    Promoter
    Available since
    March 1, 2016
    Availability
    Academic Institutions and Nonprofits only
    70321-plasmid-map-sequence-id-125770

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  34. pCDNA4-myc-His-mPGC-1a

    Plasmid
    #24925
    Depositor
    Toren Finkel
    Insert
    PGC-1a (Ppargc1a Mouse)
    Use
    Tags
    His and Myc
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    June 10, 2010
    Availability
    Academic Institutions and Nonprofits only

  37. qTAG-N-Puro-mNeon

    Plasmid
    #207687
    Purpose
    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-mNeon cassette
    Depositor
    Laurence Pelletier
    Article
    Philip et al bioRxiv
    Type
    Empty backbone
    Use
    CRISPR; Donor cassette
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Nov. 15, 2023
    Availability
    Academic Institutions and Nonprofits only
    207687-plasmid-map-sequence-id-413812

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  40. pUltra-hot

    Plasmid
    #24130
    Purpose
    3rd generation Lentiviral vector for bi-cistronic expression of mCherry and the gene of interest
    Depositor
    Malcolm Moore
    Type
    Empty backbone
    Use
    Lentiviral
    Tags
    mCherry
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 26, 2010
    Availability
    Academic Institutions and Nonprofits only
    24130-plasmid-map-sequence-id-247629

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  43. sfGFP-Caveolin-C-10

    Plasmid
    #56366
    Purpose
    Localization: Membrane, Excitation: 485, Emission: 507
    Depositor
    Michael Davidson
    Insert
    Caveolin (CAV1 Human)
    Use
    Tags
    sfGFP
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 13, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  46. pTriEx-mCherry-PA-Rac1-C450A

    Plasmid
    #22028
    Purpose
    Expression of Rac1 fused with light-insensitive LOV2 (C450A mutation)
    Depositor
    Klaus Hahn
    Article
    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.
    Insert
    PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    Use
    Tags
    6xHis and mCherry
    Expression
    Bacterial, Insect, and Mamm…
    Mutation
    LOV(Leu404-Leu546, C450A). Rac1 starts at Ile4 an…
    Promoter
    CMV, p10
    Available since
    Sept. 29, 2009
    Availability
    Academic Institutions and Nonprofits only
    22028-plasmid-map-sequence-id-213358

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  49. pDGB3_alpha1-pAtUBQ10:Myc-GVG-HA-tE9-6xUAS-35Smini-HA-LRR-NEL-mcherry-Tnos

    Plasmid
    #211842
    Purpose
    Plant transient expression cassette for a Dexamethasone-inducible E3-DART ligase
    Depositor
    Marcela Rojas-Pierce
    Article
    Huang et al Plant Cell. 2024 Mar 6:koae072. doi: 10.
    Inserts
    LRR-NEL
    GAL4-VP16-GR
    Use
    Synthetic Biology
    Tags
    HA, Myc, and mCherry
    Expression
    Plant
    Mutation
    Promoter
    6xUAS-35Smini and pUBQ10
    Available since
    March 14, 2024
    Availability
    Academic Institutions and Nonprofits only
    211842-plasmid-map-sequence-id-422272

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Showing: 8801 - 8850 of 148057 results