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pHR_SFFv_Her2-BFP_RHL027
Plasmid#164823PurposeLentiviral vector for constitutive expression of HER2+TM(23-675)-BFPDepositorInsertExtracellular and transmembrane region of ERBB2 (ERBB2 Human)
UseLentiviralTagstagBFPExpressionMutationPromoterSFFVAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDY0863 PASTEv4 pCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
Plasmid#179106PurposepCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINTDepositorInsertpCMV-SpCas9-XTEN-RT(L139P)-(GGGS)3-BceINT
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGL2-p21 promoter-Luc
Plasmid#33021DepositorInsertp21 promoter (CDKN1A Human)
UseLuciferaseTagsLuciferaseExpressionMammalianMutationPromoterp21Available sinceApril 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pKeima-LiveDrop
Plasmid#199695Purposelipophagy reporter system based on the Keima-fluorophoreDepositorInsertGPAT4 (GPAT4 Human)
UseTagsKeimaExpressionMammalianMutationPromoterCMVAvailable sinceJan. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-Neo-TRE-CMV-Cre-rtTA
Plasmid#165457PurposeTetracycline inducible CRE expression from AAVS1 locusDepositorInsertCRE
UseTagsExpressionMammalianMutationPromoterTight TRE promoterAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EJ2GFP-puro
Plasmid#44025DepositorInsertEJ2GFP egfp-based chromosomal break reporter
UseTagsExpressionMammalianMutationinsertion of I-SceI, 3 frame stop, and flanking m…PromoterpCAGGSAvailable sinceMay 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-BRAF-V600E
Plasmid#116204PurposeLentiviral expression of BRAF V600EDepositorInsertBRAF (BRAF Human)
UseLentiviralTagsExpressionMutationV600EPromoterAvailable sinceJan. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBABE-puro-hTERT
Plasmid#1771PurposeRetroviral expression of hTERT. Used to create immortalized cell lines.DepositorInserthTERT (TERT Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pLenti-DsRed_IRES_EGFP
Plasmid#92194PurposeLentiviral overexpression vector to make stable bicistronic cell line for control (EGFP) screenDepositorInsertDsRed-IRES-EGFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)_IRES_eRF1(E55D)-HA
Plasmid#182653PurposeEncodes codon-optimized AF mutant of M. mazei pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF),tRNACUAPyl & eRF1E55D used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
mutant eukaryotic release factor 1
UseTagsHA tag and nuclear export signal (NES)ExpressionMammalianMutationE55D and Y306A/Y384F (AF) double mutant of Methan…PromoterCMV and U6Available sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCW57-GFP-2A-MCS
Plasmid#71783PurposeAll-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseLentiviral; Doxycycline inducible; p2a self cleav…TagsExpressionMammalianMutationPromoterAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pDisplay-iSeroSnFR
Plasmid#128484PurposeFluorescent reporter for serotonin (mammalian expression, membrane localization tag)DepositorInsertiSeroSnFR
UseTagsIg-kappa leader, Myc, PDGFR + enhanced ER export,…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUC18/promSau/Lysostaphin
Plasmid#125766PurposeProduction of staphylolytic protein lysostaphin from a constitutive promoterDepositorInsertlysostaphin
UseTagsExpressionBacterialMutationsequence corresponding to the mature form proteinPromoterpemIK constitutive promoterAvailable sinceOct. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
mApple-TOMM20-N-10
Plasmid#54955PurposeLocalization: Outer Mitochondrial Membrane, Excitation: 568, Emission: 592DepositorInsertTOMM20 (TOMM20 Human)
UseTagsmAppleExpressionMammalianMutationPromoterCMVAvailable sinceAug. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX459V2.0-eSpCas9(1.1)
Plasmid#108292PurposepX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutationsDepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMay 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
hCMV-IE1:NLuc
Plasmid#118065PurposeTranscriptional unit for constitutive expression of NLuc in mammalian cellsDepositorInsertCMV:NLuc:bGH
UseLuciferase and Synthetic Biology; Assembly of gb2…TagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
PA-mCherry1-C1
Plasmid#54495PurposeLocalization: C1 Cloning Vector, Excitation: 400 / 504, Emission: 515 / 517DepositorTypeEmpty backboneUseTagsPA-mCherry1ExpressionMammalianMutationPromoterAvailable sinceJune 12, 2014AvailabilityAcademic Institutions and Nonprofits only -
PB-sgRNA(MS2)
Plasmid#102560PurposePiggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.DepositorTypeEmpty backboneUseCRISPR; Piggybac transposonTagsExpressionMammalianMutationPromoterU6Available sinceJan. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
Lenti-EF1alpha-dCas9-KRAB_Hygro
Plasmid#192663Purpose3rd generation lenti vector encoding dCas9-KRAB with 2A Hygro resistance markerDepositorInsertdCas9-KRAB
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, D839A, H840A and N863A in Cas9PromoterEF1aAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only
