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pCfB9342 (pgRNA_XIII-1_NatMX)

Plasmid

#161594

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XIII-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pCfB9343 (pgRNA_XV-1_NatMX)

Plasmid

#161595

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XV-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCfB9344 (pgRNA_XVI-1_NatMX)

Plasmid

#161596

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at site XVI-1

Depositor

Irina Borodina

Article

Babaei et al FEMS Yeast Res. 2021 May 11;21(4). pii:

Insert

guiding RNA

Use

CRISPR and Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

Available since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC3-TPK2

Plasmid

#176180

Purpose

yeast MoClo level-0 part plasmid type 3 containing TPK2 gene

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

TPK2 (TPK2 Budding Yeast)

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pMC8b-XI1up

Plasmid

#176166

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC7-XI1dw

Plasmid

#176167

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-1

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-1

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC8b-XI2up

Plasmid

#176168

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XI-2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC7-XI2dw

Plasmid

#176169

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XI-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XI-2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC8b-XII2up

Plasmid

#176174

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site XII-2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC7-XII2dw

Plasmid

#176175

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site XII-2

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site XII-2

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC8b-X4up

Plasmid

#176164

Purpose

yeast MoClo level-0 part plasmid type 8b containing 5' homology for integration site X-4

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

5' homology for integration site X-4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pMC7-X4dw

Plasmid

#176165

Purpose

yeast MoClo level-0 part plasmid type 7 containing 3' homology for integration site X-4

Depositor

Florian David

Article

Otto et al ACS Synth Biol. 2021 Dec 17;10(12):3461-

Insert

3' homology for integration site X-4

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEDJ-87

Plasmid

#163085

Purpose

MarkerFree plasmid for integration of pADH1-MCP-VPR into site XI-5

Depositor

Michael Jensen

Article

Jensen et al Microb Cell Fact. 2017 Mar 15;16(1):46.

Insert

VPR

Use

Tags

MCP

Expression

Yeast

Mutation

Promoter

ADH1

Available since

Sept. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pDB4953

Plasmid

#174020

Purpose

pDUAL plasmid expressing GFP-Atg6 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg6

Use

Tags

GFP, Atg6

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB4658

Plasmid

#174017

Purpose

pDUAL plasmid expressing GFP-Atg8 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg8

Use

Tags

GFP, Atg8

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB4958

Plasmid

#174014

Purpose

pHIS3H plasmid expressing CFP-Atg6 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Atg6

Use

Tags

CFP, Atg6

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB4924

Plasmid

#174012

Purpose

pDUAL plasmid expressing Pil1-mCherry from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Pil1-mCherry

Use

Tags

Pil1, mCherry

Expression

Yeast

Mutation

Promoter

41nmt1 and 41nmt1p

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pDB4948

Plasmid

#174019

Purpose

pDUAL plasmid expressing Pil1-mCherry-Vps38 from 41nmt1 promoter

Depositor

Li-Lin Du

Article

Yu et al J Cell Sci. 2021 Oct 1;134(19):jcs258774

Insert

Vps38

Use

Tags

Pil1, mCherry, Vps38

Expression

Yeast

Mutation

Promoter

41nmt1

Available since

Sept. 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pJAID5XY

Plasmid

#165051

Purpose

Integrative expression of mRFP-CUP1-AID* (mini auxin inducible degron)-yEGFP and rice auxin receptor gene OsTIR1.

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

P-URA3>KlURA3>T-AgTEF1-P-TEF1>mRFP-AID*>yEGFP>T-PGK1-P-ACS2>OsTIR1opt>T-URA3

Use

Tags

Expression

Yeast

Mutation

OsTIR1 is codon-optimised for expression in S. ce…

Promoter

Available since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)

Plasmid

#164916

Purpose

Expresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTR

Depositor

Nicholas Ingolia

Article

Muller et al Science. 2020 Dec 11;370(6522). pii: 370

Insert

GCN4(UTR)-ZEM(TF)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

PGK1 (yeast)

Available since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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