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pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

Plasmid

#223712

Purpose

Expresses autophagy flux LC3B fluorescence reporter

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

LC3B (MAP1LC3B Synthetic, Human)

Use

Lentiviral and Synthetic Biology

Tags

mCherry::EGFP::LC3B

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pRDAX-499 C-Tag GFP[1] L10 Piezo1 mCherry

Plasmid

#130062

Purpose

PPI (BiFC/TriFC) assay vector

Depositor

Gary Lewin

Insert

Piezo1 (Piezo1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR

Plasmid

#171596

Purpose

Ectodomain deleted negative control ACE2 from Bxb1 landing pad

Depositor

Kenneth Matreyek

Article

Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.

Inserts

ACE2 (ACE2 Human)
mCherry
H2A
Pac

Use

Synthetic Biology; Promoterless bxb1 dna recombin…

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 21, 2021

Availability

Academic Institutions and Nonprofits only

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pCDH-cGAS-E225A D227A-mCherry2-EF1-Puro

Plasmid

#132771

Purpose

Interacts with cytoplasmic DNA. Useful for identifying sites of nuclear envelope rupture.

Depositor

Jan Lammerding

Article

Denais et al Science. 2016 Apr 15;352(6283):353-8. do

Insert

Cyclic GMP-AMP synthase (CGAS Human)

Use

Lentiviral

Tags

mCherry2

Expression

Mammalian

Mutation

E225A D227A

Promoter

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Neo

Plasmid

#170321

Purpose

Expresses HA and mCherry tagged RPL22, neomycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pGL3-sgRab18.N-Cas9-T2A-mCherry-P2A-Puro

Plasmid

#129418

Purpose

Encoding Cas9 and sgRAB18.N for CRISPR/Cas9 mediated HDR tagging of endogenous human RAB18 N-terminus

Depositor

Elina Ikonen

Article

Salo et al Dev Cell. 2019 Jun 6. pii: S1534-5807(19

Insert

SpCas9 and sgRAB18.N (RAB18 Human)

Use

CRISPR

Tags

T2A-mCherry-P2A-Puro

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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PCDNA3-FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Plasmid

#124494

Purpose

Expresses FlipGFP (caspase-1 cleavage sequence) and T2A mCherry

Depositor

Xiaokun Shu

Article

Zhang et al J Am Chem Soc. 2019 Mar 1. doi: 10.1021/

Insert

FlipGFP(Caspase-1 cleavage seq) T2A mCherry

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV CMV-DIO-(mCherry-U6)-shRNA(anti-Crh)

Plasmid

#214732

Purpose

Expresses shRNA against CRH, marked with mCherry, in infected and cre positive cells

Depositor

Félix Leroy

Article

de Leon Reyes et al Cell. 2023 Sep 14;186(19):4152-4171.e31.

Insert

anti-Crh shRNA / mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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IGI-P0492 pHR-dCas9-NLS-VPR-mCherry

Plasmid

#102245

Purpose

Lentiviral expression of dCas9-VPR-mCherry fusion protein for CRISPRa.

Depositor

Jacob Corn

Insert

dCas9-VPR-mCherry

Use

CRISPR and Lentiviral

Tags

NLS-VPR-mCherry

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Oct. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pN1-CMV-Aff6_V18FdN-IntC-mCherryC-T2A-EGFP (C2)

Plasmid

#210725

Purpose

NIR optogenetic module for conditional protein splicing of split mCherry (C-part)

Depositor

Vladislav Verkhusha

Article

Karasev et al J Mol Biol. 2023 Dec 15;435(24):168360.

Insert

C2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

Plasmid

#112677

Purpose

An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein

Depositor

Brandon Harvey

Article

Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

Has Service

AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

Insert

Nuclear-localized floxed-mCherry EGFP

Use

AAV

Tags

Nuclear localization signal

Expression

Mutation

Promoter

EF1a

Available since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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P-Ef1a-RPL22-3XHA-P2A-mCherry-T2A-Puro

Plasmid

#170320

Purpose

Expresses HA and mCherry tagged RPL22, puromycin resistance

Depositor

Hemali Phatnani

Article

Gregory et al Cells. 2020 Jun 5;9(6). pii: cells906140

Insert

RPL22 (RPL22 Human)

Use

Lentiviral

Tags

Expression

Mutation

None

Promoter

Available since

May 17, 2021

Availability

Academic Institutions and Nonprofits only

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pSH-CAG-AtAFB2.F74A-mCherry-IRES-puro

Plasmid

#216244

Purpose

Express AtAFB2.F74A mutant with mCherry tag for AID2

Depositor

Elina Ikonen

Article

Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

Insert

AtAFB2.F74A-mCherry

Use

CRISPR, TALEN, and Unspecified ; Human safe harbo…

Tags

mCherry

Expression

Mammalian

Mutation

F74A mutation

Promoter

CAG

Available since

March 27, 2024

Availability

Academic Institutions and Nonprofits only

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RabV CVS-N2c(deltaG)-mCherry-P2A-FlpO

Plasmid

#73471

Purpose

Expresses mCherry and FlpO for monosynaptic circuit mapping and genetic manipulation

Depositor

Thomas Jessell

Article

Reardon et al Neuron. 2016 Jan 19. pii: S0896-6273(16)

Insert

mCherry-P2A-FlpO

Use

Neurotropic virus

Tags

Expression

Mutation

Promoter

Available since

June 2, 2016

Availability

Academic Institutions and Nonprofits only

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pSLQ7748 mU6: Spy sgTET || CMV: mCherry~P2A-DD-A4

Plasmid

#123657

Purpose

DD-AcrIIA4 fusion for Shield1-mediated AcrIIA4 control with sgRNA driving TRE3G activation.

Depositor

Stanley Qi

Article

Nakamura et al Nat Commun. 2019 Jan 14;10(1):194. doi:

Insert

mCherry-P2A-DD-AcrIIA4

Use

CRISPR, Lentiviral, and Synthetic Biology

Tags

mCherry-P2A

Expression

Mammalian

Mutation

Promoter

CMV and mU6

Available since

April 24, 2019

Availability

Academic Institutions and Nonprofits only

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lentiTet-RAB10-T2A-RABIF-P2A-mCherry BlastR

Plasmid

#186740

Purpose

Dox inducible RAB10-T2A-RABIF-P2A-mCherry expression construct cloned into a lentiviral backbone containing a blasticidin resistance gene

Depositor

Richard Sherwood

Article

Hamilton et al bioRxiv. 2023 Jan 10:2023.01.09.500804.

Insert

RAB10, RABIF (RAB10, RABIF Human)

Use

Lentiviral

Tags

RAB10-T2A-RABIF-P2A-mCherry

Expression

Mammalian

Mutation

Promoter

Available since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-sesRNA-smFlag-tTA2-W3SL

Plasmid

#192066

Purpose

Expression of mCherry, sesRNA in mammalian cells, with smFlag and tTA2 as efRNA.

Depositor

Josh Huang

Article

Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

Insert

sesRNA for Mouse Ctip2

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-DIO-hM3Dq-mCherry (Cre-OFF)

Plasmid

#166609

Purpose

Encodes Cre-inactivated hM3Dq-mCherry under control of the TRE

Depositor

Martin Myers

Article

Sabatini et al Elife. 2021 Mar 11;10. pii: 66835. doi:

Insert

hM3Dq-mCherry

Use

AAV

Tags

Expression

Mutation

Promoter

tetracycline response element

Available since

April 5, 2021

Availability

Academic Institutions and Nonprofits only

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CMV-RfxCas13d-SV40pA_U6-BbsI-DR_CMV-mCherry-BGHpA

Plasmid

#171380

Purpose

Expression vector for encoding a human codon-optimized RfxCas13d driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.

Depositor

Hui Yang

Article

Xu et al Nat Methods. 2021 May;18(5):499-506. doi

Insert

humanized RfxCas13d

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV, U6

Available since

July 12, 2021

Availability

Academic Institutions and Nonprofits only

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pLEX307-EF1a-ChR2[H134R]-mCherry-Puro-WPRE

Plasmid

#125256

Purpose

3rd gen lentiviral expression of humanized ChR2 with H134R mutation fused to mCherry driven by EF1a promoter for optogenetic activation with puro selection

Depositor

Roger D. Kamm

Article

Osaki et al Sci Adv. 2018 Oct 10;4(10):eaat5847. doi

Insert

hChR2(H134R)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

EF1a-forward

Available since

May 22, 2019

Availability

Academic Institutions and Nonprofits only

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