-
-
pCDNA3 T7 BimL (S58A)
Plasmid#24249DepositorInsertBim L(S58A) (BCL2L11 Human)
UseTagsT7ExpressionMammalianMutationChanged serine 58 to alaninePromoterAvailable sinceJune 1, 2010AvailabilityAcademic Institutions and Nonprofits only -
Neurl1-FLAG/Jag1-HA pIRES
Plasmid#17337DepositorInsertNeuralized-like (Neurl1a Mouse)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceApril 24, 2008AvailabilityAcademic Institutions and Nonprofits only -
Neurl1G2A-Flag pcDNA3.1
Plasmid#17320DepositorInsertNeuralized-like (Neurl1a Mouse)
UseRNAiTagsFLAGExpressionMammalianMutationGly 2 changed to AlaPromoterAvailable sinceFeb. 20, 2008AvailabilityAcademic Institutions and Nonprofits only -
p072 pCS xGATA4 + xTRPS1(C119)
Plasmid#11043DepositorUseXenopus, mammalian, avian, and zebrafishTagsExpressionMutationFusion construct of XGATA4 (amino acids 1-352) wi…PromoterAvailable sinceDec. 6, 2005AvailabilityAcademic Institutions and Nonprofits only -
-
HA-cNM2C
Plasmid#236535PurposeFor expression of NM2C (dog) in mammalian cellsDepositorInsertMYH14
UseTagsHAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2B-Flag
Plasmid#236534PurposeFor expression of NM2B (dog) - with HA and FLAG tags in mammalian cellsDepositorInsertMYH10
UseTagsFLAG and HAExpressionMammalianMutationWTPromoterAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
PGK-GFP-LAMP1TM
Plasmid#234874PurposeLentiviral plasmid expressing GFP fused to the transmembrane domain and cytosolic tail of LAMP-1. Designed as a control vector for PGK-HA-PGRN-LAMP1TM.DepositorInsertEGFP
UseLentiviralTagsLAMP-1 transmembrane domain and cytosolic tailExpressionMammalianMutationPromoterPGKAvailable sinceApril 15, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYO1125
Plasmid#235742PurposeProtein expression of GST-tagged PX domain (Cys at 2) in bacteriaDepositorInsertp40 PX domain (NCF4 Human)
UseTagsGST-TEVExpressionBacterialMutationaa 2-149 with Cys at 2PromoterAvailable sinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Venus-rab11a
Plasmid#234744PurposeMammalian expression of recycling endosome marker Venus-rab11aDepositorInsertVenus-rab11a (RAB11A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Venus-rab7a
Plasmid#234743PurposeMammalian expression of late endosome marker Venus-rab7aDepositorInsertVenus-rab7a (Rab7 Mouse)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
pB-Puro-DEST (JDW 925)
Plasmid#229808PurposeA puromycin selectable, PiggyBac destination vector compatible with 4-way multi-site gateway cloning systemDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-Dre-nls (JDW 1131)
Plasmid#229809PurposeA CAGGS driven dre recombinase followed by a c-terminal 1x nls sequenceDepositorInsertDRE recombinase-nls
UseTagsSV40 NLSExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCAGEN-WPRE-bGH-DEST (JDW 1309)
Plasmid#229815PurposeA CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcriptsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGLAST-DEST (JDW 1009)
Plasmid#229822PurposeA single site gateway compatible destination vector with an upstream GLAST promotor to drive expression in glial cells.DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 26, 2025AvailabilityAcademic Institutions and Nonprofits only -
-
-
pME-MCS (JDW 455)
Plasmid#229832PurposeA gateway compatible middle entry clone containing an MCS (mutiple cloning site).DepositorInsertattL1/L2 flanked MCS
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
pME-StayGoldc4-Giantin (JDW 1355)
Plasmid#229844PurposeA gateway compatible middle entry clone containing a V5 tagged n2StayGoldc4 flourescent proteinDepositorInsertn2StayGoldc4
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceFeb. 13, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
UseTags3xMS2ExpressionMammalianMutationPromoterSV40Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
UseTagsmCherryExpressionMammalianMutationPromoterLTRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Halo-TCAB1 HRD
Plasmid#207535PurposeHomologous recombination donor to insert HaloTag at the N-terminus of the endogenous TCAB1 locus.DepositorInsertHaloTag with internal PuroR cassette flanked by human TCAB1 locus sequences
UseTagsHaloTagExpressionMammalianMutationPromoterEndogenousAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pEJS2703-CMV-iNme2Smu-ABE8e-nt
Plasmid#228724PurposeExpresses N-terminal ABE8e-iNme2Smu nCas9 (D16A) base editor in mammalian cellsDepositorInsertN-terminal ABE8e with iNme2Smu nCas9 (D16A)
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2711-CMV-Nme2-ABE8e-i1-L10-D56R
Plasmid#228732PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and D56R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-D56R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D56RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2714-CMV-iNme2Smu-ABE8e-i1-L10
Plasmid#228735PurposeExpresses domain inlaid iNme2Smu nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid iNme2Smu nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9, Smu PID, D16A D844G E868K K870R D873A …PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2710-CMV-Nme2-ABE8e-i1-L10-E932R
Plasmid#228731PurposeExpresses domain inlaid Nme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 and E932R mutation in mammalian cellsDepositorInsertDomain inlaid Nme2 nCas9 (D16A)-i1-L10-E932R
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A E932RPromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEJS2713-CMV-iNme2-ABE8e-i1-L10
Plasmid#228734PurposeExpresses domain inlaid iNme2 nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cellsDepositorInsertDomain inlaid iNme2 nCas9 (D16A)-i1-L10
UseCRISPRTagsExpressionMammalianMutationNme2-Cas9 D16A D844G E868K K870R D873A D911G K929…PromoterCMVAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito(OM)-deltaCKAR
Plasmid#222431PurposeFRET-based reporter for monitoring deltaPKC activity at the mitochondrial outer membrane in cells.DepositorInsertMitochondrial outer membrane delta-selective C kinase activity reporter
UseTagsCFP and YFPExpressionMammalianMutationPromoterAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-Strep-Opa1 (SB251)
Plasmid#227600PurposeInducible expression of Strep-tagged Opa1 in Pichia pastorisDepositorInsertOPA1 (OPA1 Human)
UseTagsTwin-StrepTagExpressionYeastMutationcontains aa 253-960 onlyPromoterAOX1Available sinceNov. 14, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX330-PCNT
Plasmid#227284PurposeExpresses SpCas9 and a sgRNA targeting the N-terminus of PCNT for knock-in.DepositorInsertsgRNA Targeting N-terminus of PCNT (PCNT Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-PGK-Zeo
Plasmid#227271PurposeEmpty AAVS1 targeting donor for the insertion of Zeo and a medium strength PGK promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-AAVS1-Ef1a-Blast
Plasmid#227267PurposeEmpty AAVS1 targeting donor for the insertion of Blast and a strong EF1a promoter. A CDS can be cloned adjacent to the promoter via restriction or gibson cloning using the MluI cut site.DepositorTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen R119A
Plasmid#226572PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationR119APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen D188A
Plasmid#226573PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationD188APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant SigmaR1-mNeonGreen F191A
Plasmid#226571PurposeEncodes mutant siRNA resistant SigmaR1 protein (substitution) labeled with mNeonGreenDepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseTagsmNeonGreenExpressionMammalianMutationF191APromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
siRNA-resistant pLV-SigmaR1-mNeonGreen
Plasmid#226569PurposeEncodes siRNA resistant SigmaR1 protein labeled with mNeonGreen (lentiviral vector)DepositorInsertSigmaR1 (Sigma-1 Receptor) (SIGMAR1 Human)
UseLentiviralTagsmNeonGreenExpressionMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-CAGGS (JDW 912)
Plasmid#224472PurposeGateway compatible 5' entry clone containing the CAG promoter for constitutive expression of downstream constructs.DepositorInsertCAGGS Promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pX458-PLK4
Plasmid#227310PurposeExpresses SpCas9 and a sgRNA targeting the C-terminus of PLK4 for knock-in.DepositorInsertsgRNA Targeting C-terminus of PLK4 (PLK4 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
-
-
p3E-EF1a-pA (JDW 1222)
Plasmid#224526PurposeA Gateway compatible 3' entry clone containing rat EF-1a polyADepositorInsertEF-1a polyA
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJB65
Plasmid#226319PurposeE. coli protein expression for protein purificationDepositorInsert6xHis- (VTG to AAA MR1-6)-strep
UseTags6xHis and StrepExpressionBacterialMutationN-terminal domain deleted, C-terminal domain dele…PromoterT7Available sinceOct. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E_hs_Glast (JDW 1182)
Plasmid#224483PurposeGateway compatible 5' entry clone containing the Human GLAST promoterDepositorInsertGLAST promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSG069
Plasmid#214260PurposepJN105 with PaftsH2SD and PaFtsH2 ORF cloned between NheI/XbaIDepositorInserttLST accessory genetic element encoded PaFtsH2 proteinase from Pseudomonas aeruginosa SG17M
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceOct. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-EF1a (JDW 1164)
Plasmid#224473PurposeGateway compatible 5' entry clone containing the human EF1a promoterDepositorInsertEF1a promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
p5E-hs_Cldn5 (JDW 1120)
Plasmid#224481PurposeGateway compatible 5' entry clone containing the Human Claudin 5 promoterDepositorInsertClaudin-5 promoter
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME AmCyan (JDW 1151)
Plasmid#224485PurposeGateway compatible middle entry clone containing AmCyan cytosolic blue flourescent reporterDepositorInsertAmCyan
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME V5 mScarlet-I (JDW 1323)
Plasmid#224487PurposeGateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)DepositorInsertV5-mScarlet-I
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only
