p072 pCS xGATA4 + xTRPS1(C119)
(Plasmid #11043)

• Depositing Lab: Ramesh Shivdasani
• Publication: Malik et al EMBO J. 2001 Apr 2. 20(7):1715-25.

Backbone

• Vector backbone: pCS105
• Backbone size w/o insert (bp): 4133
• Vector type: xenopus, mammalian, avian, and zebrafish

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: GATA4 + TRPS1(C119)
• Alt name: GATA4
• Alt name: TRPS1
• Species: X. laevis (frog)
• Insert Size (bp): 1415
• Mutation: Fusion construct of XGATA4 (amino acids 1-352) with the terminal 119 amino acids (1153-1272) of XTRPS1
• Entrez Gene: trps1
• Entrez Gene: gata4-b (a.k.a. gata-4, gata4, xgata4)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: SP6
• 3′ sequencing primer: T3 or M13 reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Binds DNA. Fusion construct of XGATA4 with the repressive domain of XTRPS1, which encodes two Ikaros type zinc fingers.

Despite slight sequence variation, the gene is still GATA4. Xenopus being a tetraploid species and one that is not inbred, there is minor drift among alleles and our clone was probably derived from such a variant allele. The homology remains sound and the function is intact.