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Plasmid#202629Purposevector for encoding an engineered glycosylase-based guanine base editor (gGBEv6.3) with SpCas9 nickase driven by EF1a promoter, guide RNA compatible with SpCas9 driven by hU6, mCherry driven by CBh promoterDepositorInsertnSpCas9(D10A)-MPGv6.3
UseTagsExpressionMammalianMutationMPGv6.3 = G163R, N169G, D175R, C178N, S198A, K202…PromoterhU6, EF1a, CBhAvailable sinceJune 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
PGK-H2BmCherry
Plasmid#21217DepositorInsertPhosphoglycerate kinase promoter (PGK1 Human)
UseLentiviralTagsH2B-mCherryExpressionMutationPromoterAvailable sinceJuly 1, 2009AvailabilityAcademic Institutions and Nonprofits only -
pLX311-GFP-MEKDD
Plasmid#194882PurposeExpression of dominant negative MEK1DepositorInsertMEKDD (MAP2K1 Human)
UseLentiviralTagsExpressionMammalianMutationS218D, S222DPromoterE1FaAvailable sinceMarch 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3.1-FLAG-hSF3B1-K700E
Plasmid#82577PurposeExpresses a codon-optimized ORF of human SF3B1 ( K700E mutant)DepositorInserthuman SF3B1-K700E (SF3B1 Human)
UseTagsFLAG peptideExpressionMammalianMutationK700EPromoterCMVAvailable sinceMarch 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOPINB-OTUB1*
Plasmid#65441PurposeExpresses activated OTUB1 in E. coliDepositorUseTagsHis-3CExpressionBacterialMutationActive site cysteine mutated to alaninePromoterT7Available sinceJuly 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
EF1a_ASCL1_P2A_Hygro_Barcode
Plasmid#120427PurposeBarcoded lentiviral vector to express ASCL1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertASCL1 (ASCL1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCAG-D12L
Plasmid#89161PurposeExpress vaccinia virus D12L in mammalian cellsDepositorInsertVaccinia virus D12L
UseTagsExpressionMammalianMutationPromoterCAG promotorAvailable sinceApril 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CaaX(Hras)
Plasmid#108886PurposeExpression of membrane localized mCherryDepositorInsertmCherry-CaaX(Hras)
UseCan be used for mammalian expression or in vitro …TagsExpressionMammalianMutationPromoterAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHR CD19-PI3K CAR
Plasmid#113018Purposefor lentivirus production of CD19-PI3K CARDepositorInsertCD19 CAR-P intracellular PI3k recruiting domain from CD19 (CD19 Human, Synthetic)
UseLentiviralTagsmCherryExpressionMammalianMutationPromoterAvailable sinceFeb. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Mm.cargo(Cas9)
Plasmid#174865PurposeExpresses mouse Peg10 cargoRNA encoding SpCas9DepositorInsertSpCas9 flanked by MmPeg10 UTRs
UseTagsHA-SV40 NLS and SV40 NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGL3-RANGAP.C-linker.miniIAA7.3xFlag.P2A.BSD
Plasmid#216248PurposeHDR template to tag endogenous human RANGAP1 C-terminus with miniIAA7.3xFlag.P2A.BSDDepositorInsertHDR template for human RANGAP1 (RANGAP1 Human)
UseCRISPR and TALEN; Hdr templateTagsminiIAA7.3xFlag.P2A.BSDExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLV-neo-CD2-FAK
Plasmid#37013DepositorUseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceOct. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRK5-HA GST RagB 54L
Plasmid#19302DepositorInsertRas-related GTP binding B (RRAGB Human)
UseTagsGST and HAExpressionMammalianMutationT54LPromoterAvailable sinceSept. 15, 2008AvailabilityAcademic Institutions and Nonprofits only -
PX458-3xHA-en1FnCas9
Plasmid#201947PurposeMammalian expression plasmid of enhanced activity FnCas9 variant en1FnCas9 with T2A-EGFP and cloning backbone for sgRNADepositorInsert3xHA-NLS-en1FnCas9-T2A-EGFP
UseCRISPRTags3xHA, NLS, and T2A-EGFPExpressionMammalianMutationE1369R on FnCas9PromoterCbhAvailable sinceMay 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
PresentER-SIINFEKL (GFP)
Plasmid#102944PurposePresentER minigene encoding H2-Kb ligand SIINFEKL (GFP)DepositorInsertSIINFEKL (OVAL Synthetic, Chicken)
UseRetroviralTagsExpressionMammalianMutationOVA257-264PromoterLTRAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Neo-Bam E2F4
Plasmid#37966DepositorInsertE2F4 (E2F4 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pYTK067
Plasmid#65174PurposeEncodes ConR1 as a Type 5 part to be used in the Dueber YTK systemDepositorInsertConR1
UseTagsExpressionBacterialMutationPromoterAvailable sinceJune 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRx-Sth1Cas9-L5
Plasmid#140993PurposeSth1Cas9, TetR, KanR, L5Int, attP to create frameshifts and gene deletion in MycobacteriaDepositorInsertsSth1Cas9
TetR
L5 attP
L5 Int
KanR
UseUnspecified; Integrative in mycobactreiaTagsExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
HF-PX459 (V2)
Plasmid#118632PurposePlasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistanceDepositorInserthSpCas9-HF1-2A-Puro V2.0
UseCRISPRTags2A-Puro and 3XFLAGExpressionMammalianMutationAsparagine 497 to Alanine, Arginine 661 to Alanin…PromoterCbhAvailable sinceDec. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9(BB)-2A-Neo
Plasmid#127762PurposeUsed for expression of sgRNA in mammalian cellsDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJune 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLP-PIGA (pLP140)
Plasmid#168461PurposeBig-IN Landing Pad plasmid encoding pEF1a-driven mScarlet-P2A-CreERT2-P2A-PuorR-P2A-hmPIGA expression cassette flanked by heterotypic LoxM/LoxP sites and BsaI homology arms cloning sites.DepositorTypeEmpty backboneUseCre/Lox, Mouse Targeting, and Synthetic Biology ;…TagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC8 human IFN-gamma
Plasmid#17600DepositorInsertinterferon-gamma cDNA (IFNG Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc-CEBPA
Plasmid#219393PurposeExpress CEBPA in mammalian cellsDepositorInsertCEBPA (CEBPA Human)
UseTagsN terminal Myc tagExpressionMammalianMutationPromoterAvailable sinceJune 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHEA
Plasmid#168297PurposeBacterial surface display of polypeptides (nanobodies) in E. coliDepositorInsertEnterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
UseTags6xHis, E-tag, and PelB signal peptideExpressionBacterialMutationPromoterLacI-PlacAvailable sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCold CL7-Cas9
Plasmid#124890Purposeobtaining full Cas9 RNPDepositorInsertCas9
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDY1314_RADARS_Luciferase
Plasmid#193191PurposeExpress Gaussia Luciferase conditional upon RNA transcriptDepositorInsertRADARS Luciferase
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
12XCSL-d1EGFP
Plasmid#47684PurposeNotch reporterDepositorInsertTP-1 promoter
UseNotch reporter constructTagsd1EGFPExpressionMutationhexamerized 50-bp EBNA2 response element of the T…PromoterNAAvailable sinceFeb. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CAG-Electra2-P2A-EGFP
Plasmid#184936PurposeExpresses blue fluorescent protein Electra2 and EGFP in mammalian cellsDepositorInsertElectra2-P2A-EGFP
UseAAVTagsExpressionMammalianMutationPromoterCAGAvailable sinceSept. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
R4pGWB601_UBQ10p-miniTurbo-YFP-NLS
Plasmid#127370PurposeBinary vector for expressing nuclear miniTurbo-YFP under the UBQ10 promoter in plantsDepositorInsertminiTurbo (BirA mutant)
UseTagsGS linker, NLS, V5, and YFPExpressionPlantMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …PromoterArabidopsis UBQ10 promoterAvailable sinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only