Showing: 3501 - 3520 of 10430 results
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Plasmid#113044PurposeNon binary level 1 position 7 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only
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FB007
Plasmid#119705PurposePromoter gpdA from Aspergillus nidulans domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter gpdA
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromotergpdAAvailabilityAcademic Institutions and Nonprofits only -
FB008
Plasmid#119706PurposeTerminator trpC from Aspergillus nidulans domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator trpC
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB009
Plasmid#119707PurposeTranscriptional Unit (TU) for geneticin (G418) resistance in fungi obtained by combining FB001+FB005+FB002 into pDGB3alpha2. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPtrpC::nptII::Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB010
Plasmid#119708PurposeTranscriptional Unit (TU) for hygromicin resistance in fungi obtained by combining FB001+GB0211+FB002 into pDGB3alpha1. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPtrpC:hph:Ttub
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB012
Plasmid#119709PurposeComplete TU for hygromicin resistance in fungi, domesticated into pUPD2 with AATG/GCTT barcodes. Used for gene KO with dual selection, according to FungalBraid modular DNA assembly for ATMTDepositorInsertPtrpC:hph:Ttub
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB013
Plasmid#119710PurposeTU of the HSVtk negative marker for F2dU domesticated into pUPD2 with GGAG/TACT barcodes (reverse orientation). Used for gene KO with dual selec. According to FungalBraid modular DNA assembly for ATMTDepositorInsertNegative selection marker HSVtk (Pgpda:HSVtk:Ttub)
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB026
Plasmid#119711PurposeTranscriptional Unit (TU) for YFP expression, obtained by combining FB/GBparts FB007+GB0053+FB008 into pDGB3alpha1R. According to FungalBraid/GoldenBraid modular DNA assembly for ATMTDepositorInsertPromoter gpdA:YFP coding sequence:Terminator trpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB027
Plasmid#119712PurposeMultipartite assembly obtained by combining FB parts FB026+FB003 into pDGB3omega1. Used for fluorescent tagging of fungi (Hyg resistance +YFP) according to FungalBraid modular DNA assembly for ATMTDepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC::hph::Ttub (→)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB029
Plasmid#119713PurposePromoter paf from Penicillium chrysogenum domesticated into pUPD2 with GGAG/AATG barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformation.DepositorInsertPromoter paf
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterPromoter pafAvailabilityAcademic Institutions and Nonprofits only -
FB030
Plasmid#119714PurposeTerminator paf from P. chrysogenum domesticated into pUPD2 with GCTT/CGCT barcodes. According to FungalBraid/GoldenBraid modular DNA assembly for A. tumefaciens-mediated genetic transformationDepositorInsertTerminator paf
UseSynthetic Biology; Domestication of dna parts for…TagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
FB039
Plasmid#119715PurposeMultipartite assembly obtained by combining FBparts FB026+FB009 into pDGB3omega1. Used for fluorescent tagging of fungi (G418 resistance + YFP), according to FungalBraid modular DNA assembly for ATMT.DepositorInsertPgpdA::YFP::TtrpC(←)-PtrpC:nptII::Ttub (→)
UseSynthetic BiologyTagsExpressionMutationPromoterAvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac2.0
Plasmid#119872PurposeCumate-inducible expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR
sfGFP
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPveg promoter with CuO sequence and PxylR promoterAvailabilityAcademic Institutions and Nonprofits only -
pCas9CR4-VQR
Plasmid#120224PurposepCas9CR4 with VQR mutations for changing PAM site specificity to NGAN or NGNG from Kleinstiver et al. 2015DepositorInsertSpCas9-VQR
UseSynthetic BiologyTagsssrAExpressionMutationMutations D1135V/R1335V/T1337RPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pCas9CR4-NG
Plasmid#120225PurposepCas9CR4 with mutations to change PAM site specificity to NG from Nishimasu et al.DepositorInsertSpCas9-NG
UseSynthetic BiologyTagsssrAExpressionMutationMutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…PromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pCas9CR5
Plasmid#120227PurposepCas9CR4 with stop codon to prevent translation of ssrA degradation tagDepositorInsertSpCas9
UseSynthetic BiologyTagsExpressionMutationStop codon inserted before ssrA degradation tagPromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pCas9CR5-NG
Plasmid#120228PurposepCas9CR5 with mutations to change PAM site specificity to NG from Nishimasu et al.DepositorInsertSpCas9-NG
UseSynthetic BiologyTagsExpressionMutationMutations R1335V/L1111R/D1135V/G1218R/E1219F/A132…PromoterpTetAvailabilityAcademic Institutions and Nonprofits only -
pCAP03-sp44/p21
Plasmid#120232PurposeCarries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.DepositorInsertbidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailabilityAcademic Institutions and Nonprofits only -
pCAP03-act1p
Plasmid#120233PurposeCarries promoter cassette containing act1p promoter and apramycin resistance gene that can be amplified with homology sequence for refactoring.DepositorInsertpromoter cassette containing act1p promoter and apramycin resistance gene
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailabilityAcademic Institutions and Nonprofits only
Showing: 3501 - 3520 of 10430 results