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Plasmid#45216DepositorInsertHistone H2B-targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromoterT7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx10-P2A-CIBN-CAAX
Plasmid#188991PurposeA plasmid for dual expression of CRY2-mCh-superPLDx10 (an engineered PLD mutant with 10 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationF163L, K249R, G328S, G381V, G429D, D480GPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf1
Plasmid#10830DepositorInsertArf1 (ARF1 Bovine)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 5, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCD/NL-BHā1
Plasmid#41791PurposePackaging construct contains Gag and Pol; can be used with 3rd generation transfer plasmids along with a plasmid encoding RevDepositorInsertHIV-1 Gag/Pol and RRE sequences
UseTagsExpressionMammalianMutationPromoterAvailable sinceFeb. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Smac(1-60)mCherry
Plasmid#40880DepositorInsertSmac(1-60)mCherry (DIABLO Human)
UseTagsmCherryExpressionMutationPromoterCMVAvailable sinceNov. 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
mVenus-IQ6 WT
Plasmid#198201PurposeFRET acceptor: IQ6 domain of Moysin Va tagged with N-terminal mVenusDepositorInsertMYO5A IQ domain (Myo5a Mouse)
UseTagsmVenusExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
CENP-B targeted Aurora B FRET sensor Kif2 substrate
Plasmid#45214DepositorInsertCENP-B targeted Aurora B FRET sensor
UseTagsExpressionMammalianMutationPromotert7Available sinceMay 30, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX
Plasmid#188994PurposeA plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK57R, A59V, K109R, P245A, V264I, G328S, G381V, G4ā¦PromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-PhoCl-mCherry-myc
Plasmid#87692PurposeExpress PhoCl-mCherry with C-term myc tag in mammalian cellsDepositorInsertPhoCl-mCherry-myc
UseTagsmyc tagExpressionMammalianMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Stra6L-6His
Plasmid#174215PurposeStra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.DepositorInsertStra6L (Stra6l Mouse)
UseTags6-HisExpressionMammalianMutationnonPromoterCMVAvailable sinceAug. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pCDNA-CRY2-mCh-superPLDx30-P2A-CIBN-CAAX
Plasmid#188992PurposeA plasmid for dual expression of CRY2-mCh-superPLDx30 (an engineered PLD mutant with 30 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationK109R, P245A, G328S, G381V, G429DPromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
Mfn1(K88T)-10xmyc
Plasmid#26050DepositorInsertMfn1 (Mfn1 Mouse)
UseTags10xMycExpressionMammalianMutationK88T GTPase mutantPromoterAvailable sinceAug. 20, 2010AvailabilityAcademic Institutions and Nonprofits only -
WN10151
Plasmid#80441PurposeExpresses human codon-optimized dead (832A) LbCpf1DepositorInsertdead (D832A) LbCpf1
UseCRISPRTagsNLS-3xHAExpressionMammalianMutationD832APromoterAvailable sinceAug. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 RL3Sc
Plasmid#109365PurposeHuman rod opsin chimera with intracellular loop 3 from Scallop opsin with 1D4 tagDepositorInsertUseTags1D4ExpressionMammalianMutationPromoterCMVAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGP-CMV-jGCaMP8f F366H
Plasmid#204141PurposeFluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with F366H mutationDepositorInsertjGCaMP8f F366H
UseTagsN-terminal His tagExpressionMammalianMutationPhenylalanine 366 changed to histidinePromoterCMVAvailable sinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
YFP Rab4DN
Plasmid#99019PurposeLocalization and visualization of the dominant negative form of Rab4DepositorInsertRab4 DN (Rab4 Synthetic, Fly)
UseTagsYFPExpressionMammalianMutationS22N Dominant negativePromoterCMVAvailable sinceJune 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HCMVgL-FLAG (Merlin)
Plasmid#136450PurposeMammalian expression plasmid for HCMV glycoprotein L (Merlin strain) with FLAG tagDepositorInsertglycoprotein L (UL115 Human betaherpesvirus 5)
UseTagsShort SGGSG linker and FLAG tagExpressionMammalianMutationCodon-optimized for human cell expression.PromoterCMVAvailable sinceJuly 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA
Plasmid#100279PurposeIn vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutantDepositorInsertKdm6b (Kdm6b Mouse)
UseTagsFlag epitope tagExpressionMammalianMutationMutated catalytic domain (1025aa-End) (please seeā¦PromoterAvailable sinceSept. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA-CRY2-mCh-superPLDx50-P2A-CIBN-CAAX
Plasmid#188993PurposeA plasmid for dual expression of CRY2-mCh-superPLDx50 (an engineered PLD mutant with 50 times higher activity than the wild-type) and plasma membrane-tagged CIBNDepositorInsertPLD
UseTagsCAAX, CIBN, CRY2, P2A, and mCherryExpressionMammalianMutationD201G, A258T, K327R, G328S, G381V, G429D, T450APromoterCMVAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only