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Plasmid#73748PurposeExpression of GFP-PP-RPN2 (918-932) fusion in E.coliDepositorInsertRPN2 (RPN2 Human)
UseTags6x His and GFPExpressionBacterialMutationConstruct possesses only residues 918-932 of RPN2PromoterT7Available sinceApril 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
HOXC5 (human) HIS-tag pET
Plasmid#8563DepositorInsertHOXC5 (HOXC5 Human)
UseTagsHis and T7ExpressionBacterialMutationStarts at Amino Acid #2 (Ser); makes protein by W…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl V1-258 (aka VD1)
Plasmid#46173DepositorInsertVcl 1-258 (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-258 (VD1)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pETCON-DnvLB16 M40R;W41H;H42S
Plasmid#45125DepositorInsertDnvLB16 M40R;W41H;H42S
UseTagsAga2p for yeast surface display and c-myc epitope…ExpressionYeastMutationM40R;W41H;H42S to DnvLB16PromoterGALAvailable sinceJuly 8, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b-NmCas9-Flag D16A+H588A
Plasmid#109033PurposeD16A+H588A mutant of Cas9 from Nm8013 cloned into pET28b with a C-terminal Flag tag for protein expressionDepositorInsertCRISPR-associated endonuclease Cas9
UseTagsFlag TagExpressionBacterialMutationPromoterT7Available sinceMay 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET151-Vta1(280-330) S278C
Plasmid#87739PurposeBacterial expression of a VSL domain variant of S. cerevisiae Vta1DepositorInsertVacuolar protein sorting-associated protein Vta1 (VTA1 Budding Yeast)
UseTags6xHis-V5-TEVExpressionBacterialMutationdeleted amino acids 1-277, mutated Ser 278 to CysPromoterT7Available sinceApril 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET15b/ECFP-GgVcl 884-1066 (Vt)
Plasmid#46180DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsECFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXB9 (murine) HIS-tag pET
Plasmid#8541DepositorInsertHOXB9 (Hoxb9 Mouse)
UseTagsHis and T7ExpressionBacterialMutationMissing the first 13 amino acids of HOXB9; makes …PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q109E (TAC-->TAT)
Plasmid#105735PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109E (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ109EPromoterT7Available sinceMay 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q109N (TAC-->TAT)
Plasmid#105737PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q109N (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ109NPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q99E (TAC-->TAT)
Plasmid#105734PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q99E (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ99EPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET11a asyn Q79E (TAC-->TAT)
Plasmid#105732PurposeBacterial expression of mutant human alpha synucleinDepositorInsertalpha-synuclein Q79E (TAC-->TAT) (SNCA Human)
UseTagsExpressionBacterialMutationQ79EPromoterT7Available sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
HOXD13 (chicken) HIS-tag pET
Plasmid#8555DepositorInsertHOXD13 (HOXD13 )
UseTagsHis and T7ExpressionBacterialMutationcontains 10 nt 5' to ATG start; makes prote…PromoterAvailable sinceMay 30, 2006AvailabilityAcademic Institutions and Nonprofits only -
pET-22HT-MSI1(7-192)R61E
Plasmid#60353PurposeR61E mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationR61E; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Hook3 I136A, I139A, M142A
Plasmid#74617PurposeExpresses a triple mutant truncation of Hook3 in bacteria for purificationDepositorInserthuman Hook3 amino acids 1-239, I136A, I139A, M142A (HOOK3 Human)
UseTags6x His, Strep II, and superfolder GFPExpressionBacterialMutationD102A, E108APromoterT7Available sinceAug. 5, 2016AvailabilityAcademic Institutions and Nonprofits only -
p140 pETF-GST RanBP10 L301G
Plasmid#11093DepositorInsertRanBP10 (Ranbp10 Mouse)
UseTagsGSTExpressionBacterialMutationL301GPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
pETDUET1-hUCH37 (ISF1)-NFRKB(39-156)
Plasmid#61943PurposeCo-expression of hUCH37 (ISF1) and hNFRKB (39-156) in E.coliDepositorInsertsUseTags6x HISExpressionBacterialMutationResidues 2-39 deleted resulting in construct star…PromoterT7Available sinceOct. 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(166-611)
Plasmid#39338DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-10FNIII(x4)-DocI
Plasmid#58712PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsert10FNIII(x4)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/EYFP-GgVcl 884-1066 (Vt)
Plasmid#46181DepositorInsertVcl 884-1066 (VCL Chicken)
UseTagsEYFP and HisExpressionBacterialMutationaa 884-1066 (tail domain)PromoterT7Available sinceJuly 11, 2013AvailabilityAcademic Institutions and Nonprofits only