HOXD13 (chicken) HIS-tag pET
(Plasmid #8555)

• Depositing Lab: Corey Largman
• Publication: Shen et al J Biol Chem 1997 Mar 28;272(13):8198-206.

Backbone

• Vector backbone: pET28c
• Backbone manufacturer: Novagen
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXD13
• Alt name: HOX4I
• Alt name: HOX4.8
• Insert Size (bp): 1970
• Mutation: contains 10 nt 5' to ATG start; makes protein by Western
• GenBank ID: L09550
• Entrez Gene: HOXD13
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: William Holt

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Rogina et al Nuc. Acids Res. 21: 1316 (1993)