-
Plasmid#105562Purposeretrovirally express TAF2 shRNA with puro resistance and GFP markerDepositorInsertTAF2 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shTAF12.364
Plasmid#105559Purposeretrovirally express of TAF12 shRNA with puro resistance and GFP markerDepositorInsertTAF12 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
TRIN-shREN
Plasmid#105586PurposeDox-inducible mir30 shREN (control shRNA)/dsRED expression with Venus marker and Neo resistanceDepositorInsertcontrol shRNA targeting luciferase
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCFD5-frame_selector_0,1,2
Plasmid#131152PurposeDrosophila expression of frame selector sgRNAs 0,1, and 2 that target the CRISPaint site for homology-independent knock-inDepositorInsertCRISPaint frame selector sgRNAs 0,1,2
UseCRISPRTagsExpressionInsectMutationPromoterdU6:3Available sinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtPT4Pro
Plasmid#159414PurposeMedicago truncatula PT4 promoter sequence (836 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtPT4 Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pL0-MtBCP1Pro
Plasmid#159415PurposeMedicago truncatula BCP1 promoter sequence (1108 bp immediately upstream of start codon) in pICH41295 MoClo Golden Gate level 0 acceptor for Pro+5U modules.DepositorInsertMtBCP1 Promoter
UsePuc19-derivedTagsExpressionBacterialMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
LEP-shREN
Plasmid#105580Purposeretrovirally express control shRNA with puro resistance and GFP markerDepositorInsertcontrol shRNA targeting luciferase
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
LEP-shMLL-AF9.643
Plasmid#105565Purposeretrovirally express MLL-AF9 shRNA with puro resistance and GFP markerDepositorInsertshRNA targeting MLL part of MLL-AF9 fusion
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterMSCV-LTRAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 (RNAi-res)
Plasmid#136645PurposeExpression of SH3BP1 in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1 (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTTCPromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGG184
Plasmid#165604PurposeReporter plasmid for B1H-dependent expression of the HIS3/GFP operon with a single hEGFP protospacer: PS1(upstream of promoter with 'CAGCG' PAM)-lac-HIS3 and GFPDepositorInserthEGFP protospacer ('CAGCG' PAM) upstream of the HIS3/GFP promoter
UseSynthetic BiologyTagsExpressionBacterialMutation'CAGCG' PAM replacing the 'CGGCG…PromoterlacAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-TO-HA-SH3BP1 R312A (RNAi-res)
Plasmid#136646PurposeExpression of SH3BP1R12A in mammalian cells; resistant to siRNA targeting GAUGACAGCCACCCACUUCDepositorInsertSH3BP1R312A (RNAi resistant) (SH3BP1 Human)
UseTagsHA (MQDLPGNDNSTAG)ExpressionMammalianMutationGATGACAGCCACCCACTTC changed to AATGACAGCAACACACTT…PromoterAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRIN-E-shREN
Plasmid#105585PurposeDox-inducible mirE shREN(control shRNA)/dsRED expression with Venus marker and Neo resistanceDepositorInsertcontrol shRNA targeting luciferase
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
PGL3-U6-RNF2_pegRNA-Csy4RS-nick_sgRNA-EGFP
Plasmid#172669PurposeFor expression of transcript containing pegRNA and nick-sgRNA targeting human RNF2 gene from the U6 promoter with pegRNA flanked by Csy4 recognition siteDepositorInsertRNF2 pegRNA and RNF2_nick-sgRNA
UseTagsExpressionMammalianMutationPromoterU6Available sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pU6-sgRNA EF1Alpha-puro-T2A-BFP.CYRANO
Plasmid#128755PurposeExpresses CYRANO gRNA for CRISPRiDepositorInsertCYRANO sgRNA (OIP5-AS1 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromotermU6Available sinceAug. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
TRIN-shMYB.2572
Plasmid#105574PurposeDox-inducible mir30 MYB shRNA/dsRED expression with Venus marker and neo resistanceDepositorInsertMYB shRNA #2572
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMarch 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
-
pSUPER.retro.puro-shMePCE
Plasmid#113536PurposeRetroviral vector designed to knock down MePCE.DepositorInsertshMePCE (MEPCE Human)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT2/sh-FYN1-GFP_Seq_C
Plasmid#201399PurposeKnockdown of FYN tyrosine kinase. Construct has inverted repeats to be used in Sleeping Beauty transposon system.DepositorInsertFYN
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
p3E-U6a-U6c-opa1 guide
Plasmid#121995PurposeAn entry vector with U6a and U6c promoter driving opa1 guide RNAs expressionDepositorInsertopa1 gRNA
UseCRISPRTagsExpressionMutationPromoterAvailable sinceSept. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
TRIN-E-shTAF12.364
Plasmid#105572PurposeDox-inducible mir30 MYB shRNA/dsRED expression with GFP marker and neo resistanceDepositorInsertTAF12 shRNA
UseRNAi and RetroviralTagsExpressionMammalianMutationPromoterTREAvailable sinceMay 4, 2018AvailabilityAcademic Institutions and Nonprofits only