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Plasmid#175014PurposeNegative-control construct for Akt-STOPS.DepositorInsertmCherry-Akt-Scramble
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJan. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCherry-CD81-10
Plasmid#55012PurposeLocalization: Tetraspanin/Membrane, Excitation: 587, Emission: 610DepositorInsertCD81 (CD81 Human)
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAH123
Plasmid#66077PurposeThe pAH123 plasmid [Haldimann2001] is the phi80 helper plasmid necessary for the genome integration of the burden monitor phi80 versionDepositorInsertphi80 integrase
UseTagsExpressionMutationPromoterAvailable sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pL-CRISPR.EFS.GFP
Plasmid#57818PurposeLentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA. Coexpresses eGFP via P2A cleavage site. EFS Promoter drivenDepositorInsertsUseCRISPR and LentiviralTagsFLAGExpressionMutationPromoterEFS and hU6Available sinceAug. 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
CMV-LUC2CP/ARE
Plasmid#62857PurposeSplicing reporter control (no intron) with elements to shorten the half-life of the luciferase protein as well as the luciferase mRNA.DepositorInsertLuciferase
UseLuciferaseTagsExpressionMammalianMutationdestabilizing sequences (DS) added to the C termi…PromoterCMVAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKII-Cre
Plasmid#182736PurposeCre expression under the control of CamKII promoterDepositorInsertCre
UseAAVTagsExpressionMutationPromoterCamKIIAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
AAV sgRNA Expression Plasmid
Plasmid#174540PurposeContains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.DepositorTypeEmpty backboneUseAAVTagsNoneExpressionMammalianMutationPromoterCMVAvailable sinceNov. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGRG36
Plasmid#16666DepositorInsertmTn7::MCS
UseTagsExpressionBacterialMutationPromoterAvailable sinceJan. 10, 2008AvailabilityAcademic Institutions and Nonprofits only -
3xnls-mNeonGreen
Plasmid#98875PurposeIn vivo visualization of the nucleus (can be used for colocalization studies)DepositorArticleInsert3xNLS
UseTagsmNeonGreenExpressionMammalianMutationPromoterCMVAvailable sinceMarch 7, 2019AvailabilityAcademic Institutions and Nonprofits only -
p2706-pHAGE-EF1aL-YAP5SA-UBC-GFP
Plasmid#216475Purposelentiviral dual expression of YAP5SA and eGFPDepositorInsertsYAP5SA
eGFP
UseLentiviralTagsExpressionMutationS61A, S109A, S127A, S164A, S381APromoterEF1aL and UBCAvailable sinceApril 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
LentiGuide_DKO
Plasmid#183193PurposePlasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screenDepositorTypeEmpty backboneUseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterhU6, mU6Available sinceAug. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
p2CT-His-MBP-Lwa_Cas13a_WT
Plasmid#91865PurposeBacterial expression for Cas13aDepositorInsertLwaCas13a
UseCRISPRTagsHis6-MBP-TEV siteExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
NOVA1 eGFP
Plasmid#61275PurposeGFP-tagged ORF clone of Homo sapiens neuro-oncological ventral antigen 1 (NOVA1), transcript variant 1DepositorInsertNOVA1 (NOVA1 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 11, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMSCVhygro-Cre
Plasmid#34565DepositorInsertCre
UseRetroviralTagsExpressionMammalianMutationPromoter5'LTRAvailable sinceMarch 28, 2012AvailabilityAcademic Institutions and Nonprofits only -
pDESTmycIGF2BP3
Plasmid#19879DepositorInsertInsulin-like growth factor 2 mRNA binding protein 3 (IGF2BP3 Human)
UseTagsMYCExpressionMammalianMutationPromoterAvailable sinceDec. 31, 2008AvailabilityAcademic Institutions and Nonprofits only -
pMSCVpuro-Mito-Pericam
Plasmid#87381PurposeRetroviral transfection of cells to express ratiometric Pericam as described by Nagai et al 2001 (PMID 11248055) localising to the mitochondrial matrix via the transit peptide from human COX8A.DepositorInsertMito-Pericam [ratiometric]
UseRetroviralTagsExpressionMammalianMutationPromoterRetroviral 5' LTR promoterAvailable sinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMT525
Plasmid#131392PurposeL1RP retrotransposition reporterDepositorInsertL1RP GFP-AI
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
rnLbCas12a-PE2
Plasmid#213746PurposeFor circular RNA-mediated prime editor using rnLbCas12a-PE2 in HEK293T cellsDepositorInsertrnLbCas12a-M-MLV RT∆RNase H
UseCRISPRTagsBPNLSExpressionMammalianMutationD156R, H759A, R1138A in LbCas12aPromoterCMVAvailable sinceMarch 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
mNeonGreen-Paxillin
Plasmid#176106PurposeFocal Adhesion markerDepositorInsertPaxillin (PXN Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
PiggyBac-TetO-En1-t2a-Foxa2-hygro
Plasmid#176483PurposeTetO-En1-t2a-Foxa2 in a PiggyBac vector with hygromycin resistanceDepositorUseTagsExpressionMammalianMutationPromoterTetOAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBO.ACT001
Plasmid#182711PurposeaTc inducible dCasRxDepositorInsertdCasRx
UseCRISPRTags2x-FLAGExpressionBacterialMutationCatalytically deactivated R295A, H300A, R849A, H8…PromoterpTetAvailable sinceAug. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUltra-sY
Plasmid#82417PurposeExpresses Mj aaRS for sulfotyrosine and Mj tRNA for decoding UAG codonsDepositorInsertMethanococcus jannaschii aaRS for sulfotyrosine
UseTagsExpressionBacterialMutationTyr32Leu, Leu65Pro, Asp158Gly, Ile159Cys, Leu162L…PromotertacIAvailable sinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
mCherry-WASH1-C-18
Plasmid#55162PurposeLocalization: Cytoskeleton, Excitation: 587, Emission: 610DepositorInsertWASH1
UseTagsmCherryExpressionMammalianMutationPromoterCMVAvailable sinceSept. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRE recombinase
Plasmid#62730PurposeExpresses Cre recombinase protein in bacterial cellsDepositorInsertCre recombinase
UseCre/LoxTagsHis tag and NLSExpressionBacterialMutationCodon optimazed for e.coliPromoterT7Available sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMiniT:GFP-Gephyrin TKIT donor
Plasmid#169433PurposeDonor DNA for TKIT knockin of GFP to Gephyrin in mouseDepositorInsertEGFP
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pT076
Plasmid#137880PurposeDonor vector for integration at the human AAVS1 safe harbor locus of Doxicycline-inducible dCas9-VPR-T2A-EGFPDepositorInsertTRE3G-dCas9-VPR-T2A-EGFP-CAG-TetON-NeoR-SA
UseAAVTagsExpressionMammalianMutationPromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1_hHNF4alpha
Plasmid#206878PurposeThe ORF of HNF4α (variant 2) cDNA amplified from the RT human liver RNA was cloned at the Bam HI/XbaI sites of the pcDNA3.1(+), the mammalian expression vector.DepositorInsertHuman hepatocyte nuclear factor 4 alpha (HNF4A Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNHA_OROV_NCP
Plasmid#208937PurposeExpresses N-terminally HA-tagged Oropouche orthobunyavirus nucleocapsid protein from CMV promoter in mammalian cellsDepositorInsertNucleocapsid
UseTagsHAExpressionMammalianMutationDeleted start codon so HA included at N-terminusPromoterCMVAvailable sinceJan. 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
phND2-N106
Plasmid#31780DepositorInsertNeuroD2 (NEUROD2 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
epB_UbC_CymRV5-nls-GFP_DEx2
Plasmid#119906PurposeePiggyBac mammalian expression plasmid for CymR-GFP fusionDepositorInsertCymRV5-nls-GFP
UseTagsExpressionMammalianMutationPromoterAvailable sinceJune 6, 2019AvailabilityAcademic Institutions and Nonprofits only