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Showing: 3461 - 3480 of 10430 results

  4. IL1RN gRNA1

    Plasmid
    #113130
    Purpose
    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)
    Depositor
    Dominik Niopek
    Article
    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s
    Insert
    IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    Use
    AAV, CRISPR, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6 for gRNA expression, RSV for GFP expression
    Availability
    Academic Institutions and Nonprofits only
    113130-plasmid-map-sequence-id-217998

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  13. pRYG

    Plasmid
    #113642
    Purpose
    Positive control: shows the ratio of GFP signal to RFP signal with a tyrosine residue in the linker that connects the two genes.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113642-plasmid-map-sequence-id-214560

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  14. pRXG

    Plasmid
    #113643
    Purpose
    This construct contains an amber stop codon within the linker between the two genes. The read through rate of the amber stop codon can be determined using the ratio of GFP signal to RFP signal.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    mRFP1-linker-sfGFP
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Academic Institutions and Nonprofits only
    113643-plasmid-map-sequence-id-217116

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  15. pRF0G-Tyr

    Plasmid
    #113644
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    MjYRS and tRNA gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    113644-plasmid-map-sequence-id-217129

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  16. pRF0G-IodoY

    Plasmid
    #113645
    Purpose
    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging iodotyrosine at amber stop codons.
    Depositor
    Jeffrey Barrick
    Article
    Monk et al ACS Synth Biol. 2016 Sep 20.
    Insert
    IodoYRS and tRNA gene
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Availability
    Industry, Academic Institutions, and Nonprofits
    113645-plasmid-map-sequence-id-217127

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Showing: 3461 - 3480 of 10430 results