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PSD-93/Chapsyn-110 scFv [N18/28]

Plasmid

#190535

Purpose

Mammalian Expression of PSD-93/Chapsyn-110 scFV. Derived from hybridoma N18/28.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

PSD-93/Chapsyn-110 (Rattus norvegicus) recombinant scFV (Dlg2 Mouse)

Use

Tags

HA, Sortase, 6xHis

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2022

Availability

Academic Institutions and Nonprofits only

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shMEK1/2 puro

Plasmid

#72567

Purpose

Hairpin targeting MEK1 and MEK2 (murine).

Depositor

Kevin Janes

Article

Jensen et al Cell Syst. 2016 Feb 24;2(2):112-121.

Insert

MEK1/2 (Map2k1, Map2k2 Mouse)

Use

Lentiviral

Tags

None

Expression

Mammalian

Mutation

Promoter

Available since

July 12, 2016

Availability

Academic Institutions and Nonprofits only

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pCbeta EV (PKA catalytic subunit Cbeta)

Plasmid

#15311

Depositor

Stanley McKnight

Article

Uhler et al J Biol Chem. 1987 Nov 5. 262(31):15202-7

Insert

PRKACB (Prkacb Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 6, 2007

Availability

Academic Institutions and Nonprofits only
pRc/CMV2 Dvl-2-Myc

Plasmid

#42194

Depositor

Robert Lefkowitz, Shin-ichi Yanagawa

Article

Lee et al J Biol Chem. 1999 Jul 23;274(30):21464-7

Insert

Dvl 2 (Dvl2 Mouse)

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2013

Availability

Academic Institutions and Nonprofits only
pLNCX2-NGN2-IRES-GFP-T2A-Sox11

Plasmid

#90213

Purpose

To convert human skin fibroblasts into induced cholinergic neurons (hiCN) in combination with FGF2 and two small molecules, forskolin and dorsomorphin.

Depositor

Chun-Li Zhang

Article

Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

Insert

NGN2-IRES-GFP-T2A-Sox11 (Sox11, NEUROG2 Mouse, Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-mApg5

Plasmid

#22958

Depositor

Noboru Mizushima

Article

Mizushima et al J Cell Biol. 2001 Feb 19. 152(4):657-68.

Insert

autophagy related genes 5 (Atg5 Mouse)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 24, 2010

Availability

Academic Institutions and Nonprofits only

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pBS mCD4

Plasmid

#14613

Depositor

Dan Littman

Article

Landau et al Nature. 1988 Jul 14. 334(6178):159-62.

Insert

CD4 (Cd4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 27, 2007

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Lis1

Plasmid

#12574

Depositor

Li-Huei Tsai

Article

Shu et al Neuron. 2004 Oct 14. 44(2):263-77.

Insert

Lis1 (Pafah1b1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only

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pCMV-mA3-BE3

Plasmid

#113419

Purpose

Expresses mA3-BE3 in mammalian cells

Depositor

Jia Chen

Article

Wang et al Nat Biotechnol. 2018 Aug 20. pii: nbt.41

Insert

mA3-BE3 (Apobec3 S. pyogenes and Bacteriophage PBS2, Mouse)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pLVX-Tight-Puro_Nkx6.2

Plasmid

#64846

Purpose

A lentiviral vector for the expression of mouse Nkx6.2 under doxycyline control

Depositor

Paul Tesar

Article

Najm et al Nat Biotechnol. 2013 May;31(5):426-33. d

Insert

Nkx6.2 (Nkx6-2 Mouse)

Use

Lentiviral

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

May 28, 2015

Availability

Academic Institutions and Nonprofits only

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pBABEpuro WT ATG3

Plasmid

#26926

Depositor

Jayanta Debnath

Article

Radoshevich et al Cell. 2010 Aug 20. 142(4):590-600.

Insert

autophagy-related 3 (yeast) (Atg3 Mouse)

Use

Retroviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 31, 2011

Availability

Academic Institutions and Nonprofits only
Anti-HCN2 [N71/37R]

Plasmid

#177567

Purpose

Mammalian Expression Plasmid of anti-HCN2 (Rat). Derived from hybridoma N71/37.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

anti-HCN2 (Rattus norvegicus) recombinant mouse monoclonal antibody (Hcn2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Lem2

Plasmid

#120245

Purpose

Expresses V5-tagged Lem2 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Lem2 (Lemd2 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-IRES-hCD2-CD14

Plasmid

#133061

Purpose

Retroviral expression of mouse CD14

Depositor

Jonathan Kagan

Article

Tan et al Immunity. 2015 Nov 17;43(5):909-22. doi:

Insert

CD14 (Cd14 Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pENTR-L5-Kir2.1-mCherry-L2

Plasmid

#32669

Depositor

Matthew Nolan

Article

White et al Front Mol Neurosci. 2011;4:8. Epub 2011

Insert

Kir2.1-mCherry

Use

Gateway cloning vector

Tags

Expression

Mutation

Promoter

Available since

March 22, 2012

Availability

Academic Institutions and Nonprofits only

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pColdI-GST-mGTSF1l

Plasmid

#214817

Purpose

Bacterial expression of GST-tagged mouse GTSF1l

Depositor

Phillip Zamore

Article

Arif et al Nature. 2022 Aug;608(7923):618-625. doi:

Insert

GTSF1l (Gtsf1l Mouse)

Use

Tags

6x His and GST

Expression

Bacterial

Mutation

Promoter

CspA

Available since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2–mPKCε-CAT-HA

Plasmid

#190483

Purpose

Expresses a fluorescently-tagged, optogenetically activated PKC-epsilon. Used in tandem with CIBN-CAAX

Depositor

Diomedes Logothetis

Article

Gada et al J Biol Chem. 2022 May;298(5):101893. doi

Insert

mCherry-CRY2–mPKCε-CAT-HA (Prkce Synthetic, Mouse)

Use

Tags

Fused to CRY2, HA, and mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pSIN-Ski-gRNA2

Plasmid

#180369

Purpose

targeting mouse Ski gene

Depositor

Dan Littman

Article

Xu et al J Exp Med. 2021 Nov 1;218(11). pii: 2126

Insert

Ski targeting gRNA (Ski Mouse)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

human U6

Available since

June 10, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mNRDc-V5

Plasmid

#51297

Purpose

expression of mouse NRDc in mammalian cells

Depositor

Eiichiro Nishi

Article

Hiraoka et al Nat Commun. 2014;5:3224. doi: 10.1038/nc

Insert

NRDc (Nrd1 Mouse)

Use

Tags

HIS and V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCX-Klf2

Plasmid

#66655

Purpose

For expression of mouse Klf2.

Depositor

Barak Cohen

Article

Fiore et al Genome Res. 2016 Jun;26(6):778-86. doi:

Insert

Klf2 (Klf2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 25, 2015

Availability

Academic Institutions and Nonprofits only

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