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Plasmid#61420PurposeExpresses human OTUB1 (full-length) in E. coli.DepositorInsertOTUB1 (Ubiquitin thioesterase OTUB1) (OTUB1 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pT2K-CAGGS-T2TP
Plasmid#114725Purposeexpression in mammalian cellsDepositorInsertTol2 transposase
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET30a-Spike-E
Plasmid#165096Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 spike protein (S SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 11, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
Cer(1-158)-RGS7
Plasmid#55760PurposeAn amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.DepositorInsertmCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
UseTagsCer(1-158) was fused to the amino terminus of RGS…ExpressionMammalianMutationRGS7 was amplified via PCR, which added an N-term…PromoterCMVAvailable sinceJuly 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
MSI2-DCD
Plasmid#166968PurposeExpress the fusion protein of human MSI2 and the catalytic dead domain of Drosophila ADARDepositorInsertMSI2-DCD
UseRetroviralTagsMSI2-DCDExpressionMammalianMutationN495SPromoterMSCVAvailable sinceMarch 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWPI-LUC7L2-3xFLAG
Plasmid#201641PurposeOver-expression of 3xFLAG-tagged human LUC7L2DepositorInsertLUC7L2 (LUC7L2 Human)
UseLentiviralTags3xFLAGExpressionMutationPromoterAvailable sinceAug. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
HA-mDyn2 pcDNA3
Plasmid#36264DepositorInsertDynamin 2 (Dnm2 Mouse)
UseTagsHAExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceApril 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-A2UCOE-EF1a-iCasp9-P2A-Crry-T2A-mCD59-E2A-Qa1 SCT-F2A-neo-AAVS1
Plasmid#205445Purposehuman AAVS1-targeting plasmid for expression of iCasp9, Crry, mouse CD59, and Qa1 SCTDepositorInsertiCasp9-Crry-mCD59-Qa1 SCT
UseTagsExpressionMammalianMutationPromoterEF1aAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE
Plasmid#73438PurposeProdeoxyviolacein pathway (vioABE) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variant G6.DepositorInsertPG6-vioABE
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6 (orthogonal T7-lac variant)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
TRE-UBC-mCD55-T2A-GFP
Plasmid#205450Purposelentiviral plasmid for expression of mouse CD55DepositorInsertmCD55 (Cd55 Mouse)
UseTagsExpressionMammalianMutationPromoterUBCAvailable sinceSept. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-mouse-full-length-CHMP3-HA
Plasmid#154176Purposeexpresses mouse CHMP3 in mammalian cellsDepositorInsertCHMP3 (Chmp3 Mouse)
UseTagsHAExpressionMammalianMutationPromoterEF1alphaAvailable sinceOct. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD6B (full-length, aa 1-293)
Plasmid#61418PurposeExpresses human OTUD6B (full-length) in E. coli.DepositorInsertOTUD6B (OTUD6B Human)
UseTagsHis6-GST-3CExpressionBacterialMutationPromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
hEF1α-H2B-mVenus-IRES-mCherry PGK-Puromycin
Plasmid#99278PurposeDual fluorescent reporter - constitutive expression of mVenus (515/528) localized in the nucleus, mCherry (587/610) untargeted and visualized in the cytoplasm, and Puromycin resistanceDepositorInsertsH2B-mVenus-IRES-mCherry
Puro
UseLentiviralTagsH2B, IRES, PGK, mCherry, and mVenusExpressionMutationPromoterEF1 alpha-human and PGK-mouseAvailable sinceNov. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pRepair-mScI-CTNNB1
Plasmid#153431PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with mScarlet-iDepositorInsertCTNNB1 homology arms and mScI coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsmScarlet-iExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET30a-SARS-CoV2-NP-Nt(47-173)
Plasmid#165091Purposeprotein expression in E. coliDepositorInsertSevere acute respiratory syndrome coronavirus 2 nucleocapsid protein N-terminal(47-173) (N SARS-CoV2)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 22, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
AttB_ACE2[dEcto]_IRES-mCherry-H2A-P2A-PuroR
Plasmid#171596PurposeEctodomain deleted negative control ACE2 from Bxb1 landing padDepositorInsertsUseSynthetic Biology; Promoterless bxb1 dna recombin…TagsExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDONR-53BP1 1-1972 WT
Plasmid#53446PurposeRecombinational donor/master vectorDepositorInsert53BP1 (TP53BP1 Human)
UseGatewayTagsExpressionMutationsiRNA resistant and mutations A128V, T720A, A1347…PromoterAvailable sinceApril 10, 2015AvailabilityIndustry, Academic Institutions, and Nonprofits -
pRepair-SGFP2-CTNNB1
Plasmid#153430PurposeHomology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SGFP2DepositorInsertCTNNB1 homology arms and mTurquoise2 coding sequence (CTNNB1 Human)
UseCRISPR; Hdr donor templateTagsSGFP2ExpressionMutationPromoterAvailable sinceSept. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-OTUD5 (OTU, aa 171-358)
Plasmid#61415PurposeExpresses human OTUD5 (OTU domain) in E. coli.DepositorInsertOTUD5 (OTUD5 Human)
UseTagsHis6-GST-3CExpressionBacterialMutationDeleted aa 1-170 and aa 359-571.PromoterT7Available sinceMarch 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCAP03-sp44/p21
Plasmid#120232PurposeCarries bidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites that can be amplified with homology sequence for refactoring.DepositorInsertbidirectional promoter cassette containing sp44 and p21 promoters and apramycin resistance gene flanked by two FRT sites
UseSynthetic BiologyTagsExpressionMutationaac(3)IV (apramycin resistance gene)PromoterAvailable sinceMarch 21, 2019AvailabilityAcademic Institutions and Nonprofits only