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CMVTCRa_candidate8

Plasmid

#165005

Purpose

Expression of candidate TCR #8 TCRalpha cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

TRAV (TRA Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pUC19 KGA HA5'-mKO2-P2A-Zeo-3'

Plasmid

#110408

Purpose

Homology donor for glutaminase KGA isoform knock-in, mKO2-P2A-Zeo

Depositor

Sandra Martha Gomes Dias

Insert

GLS glutaminase (GLS Human)

Use

CRISPR

Tags

Expression

Mutation

Promoter

none

Available since

Feb. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCW-SPIC

Plasmid

#162825

Purpose

Doxycycline-inducible overexpression of human SPIC

Depositor

Matthew Steinhauser

Article

Fazeli et al Mol Metab. 2020 Sep 28;42:101082. doi: 1

Insert

SPIC (SPIC Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Tet ON

Available since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

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p667-UBC-HIPK3-V5-miniTurbo_IDG-K

Plasmid

#158191

Purpose

Gateway destination clone of HIPK3 (human) tagged with C-terminal V5-miniTurbo for generating protein-proximity networks using proximity-dependent biotinylation proteomics

Depositor

Ben Major

Insert

HIPK3-V5-miniTurbo (HIPK3 Human)

Use

Lentiviral; Gateway destination

Tags

V5-miniTurbo

Expression

Mammalian

Mutation

Promoter

Ubiquitin

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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Tol2 8xSTAR-sTomato. PGK-puro

Plasmid

#136264

Purpose

Fluorescent reporter for intestinal stem cells through ASCL2 transcriptional activity

Depositor

Hugo Snippert

Article

Heinz et al STAR Protoc. 2020 Oct 8;1(3):100126. doi

Insert

stem cell Ascl2 reporter, 8 repeats

Use

Tol2 transposase

Tags

sTomato

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-10A.12D-48-3xFLAG

Plasmid

#130678

Purpose

Expresses human SMCHD1 Exon1-10A.12D-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-10A.12D-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D409_F489del

Promoter

CMV

Available since

June 24, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-36.47M-48-3xFLAG

Plasmid

#130680

Purpose

Expresses human SMCHD1 Exon1-36.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-36.47M-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

D1523_G1960del

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-46.47A-48-3xFLAG

Plasmid

#130677

Purpose

Expresses human SMCHD1 Exon1-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-46.47A-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

M1961_E1992del

Promoter

CMV

Available since

June 22, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-REG1-3'UTR

Plasmid

#136052

Purpose

REG1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (ATTGTATCTCTGTAGTTTAAG)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

REG1 (ZC3H12A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-UPF3A-3'UTR

Plasmid

#136042

Purpose

UPF3A shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GACGTAGAAACACGCAGAAAC)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF3A (UPF3A Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-3xHA-SMCHD1-Exon1-14.47M-48-3xFLAG

Plasmid

#130691

Purpose

Expresses human SMCHD1Exon1-14.47M-48 with an N-terminal 3xHA tag and a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)

Use

Tags

3xFLAG and 3xHA

Expression

Mammalian

Mutation

E653_G1960del

Promoter

CMV

Available since

Dec. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-46.47A-48-3xFLAG

Plasmid

#130689

Purpose

Expresses human SMCHD1 Exon37-46.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-46.47A-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, M1961_E1992del

Promoter

CMV

Available since

Nov. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-9.47M-48-3xFLAG

Plasmid

#130686

Purpose

Expresses human SMCHD1 Exon1-9.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.47M-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_G1960del

Promoter

CMV

Available since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon37-41S.44R-48-3xFLAG

Plasmid

#130690

Purpose

Expresses human SMCHD1 Exon37-41S.44R-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon37-41S.44R-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

1M_T1522delinsM, G1720_Y1846del

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-9.41-48-3xFLAG

Plasmid

#130685

Purpose

Expresses human SMCHD1 Exon1-9.41-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.41-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_Q1684del

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-SMCHD1-Exon1-14.47M-48-3xFLAG

Plasmid

#130682

Purpose

Expresses human SMCHD1 Exon1-14.47M-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-14.47M-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

E653_G1960del

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pLenti-CMV-SMCHD1-Exon1-9.47A-48-3xFLAG

Plasmid

#130687

Purpose

Expresses human SMCHD1 Exon1-9.47A-48 with a C-terminal 3xFLAG tag

Depositor

Stephen Tapscott

Article

Hiramuki et al Skelet Muscle. 2018 Aug 2;8(1):24. doi:

Insert

SMCHD1 Exon1-9.47A-48 (SMCHD1 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

I378_E1992del

Promoter

CMV

Available since

Sept. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-RtcAshRNA1

Plasmid

#126613

Purpose

Expresses shRNA against human RtcA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

RtcA shRNA-1

Use

RNAi

Tags

Expression

Mutation

Promoter

mouse U6 promoter

Available since

June 12, 2019

Availability

Academic Institutions and Nonprofits only

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pB-puro-mU6-RtcAshRNA2

Plasmid

#126614

Purpose

Expresses shRNA against human RtcA under mouse U6 promoter

Depositor

Xiaozhong Wang

Article

Unlu et al J Biol Chem. 2018 Dec 14;293(50):19365-1

Insert

RtcA shRNA-2

Use

RNAi

Tags

Expression

Mutation

Promoter

Mouse U6 promoter

Available since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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Fuw CBX8 R19A

Plasmid

#121112

Purpose

conditional expression

Depositor

Emily Dykhuizen

Article

Connelly et al Nucleic Acids Res. 2018 Dec 29. pii: 526

Insert

CBX8 (R19A) (CBX8 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

R19A

Promoter

Available since

Feb. 13, 2019

Availability

Academic Institutions and Nonprofits only

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