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Plasmid#198509PurposeExpresses optimized PdCO in frame with mScarlet under control of human synapsin1 promotor after Cre-dependent recombination.DepositorInsertPdCO
UseAAV and Cre/LoxTagsRho1D4 and mScarletExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa(0.4)-PdCO-mScarlet-WPRE
Plasmid#198508PurposeExpresses optimized PdCO in frame with mScarlet under control of minimal CamKIIa promotor.DepositorInsertPdCO
UseAAVTagsRho1D4 and mScarletExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NES
Plasmid#133022PurposeNES sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorInsertMTHFD1 (MTHFD1 Human)
UseTagsNESExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCG_SARS-CoV2/bat-CoV RaTG13 E C-V5-tag
Plasmid#179977PurposeMammalian expression vector for SARS-CoV-2 E or bat RaTG13 E, V5-tagged (SARS-CoV-2 E and RaTG13 E have the same amino acid sequence and codon optimized sequence).DepositorInsertSARS-CoV-2 E (E Synthetic)
UseTagsExpressionMammalianMutationhuman codon-optimizedPromoterAvailable sinceFeb. 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_N-DYK_MTHFD1-NLS
Plasmid#133023PurposeNLS sequence was fused to MTHFD1-wild type through site-directed mutagenesis (Q5 Site-Directed Mutagenesis Kit, New England Biolabs)DepositorInsertMTHFD1 (MTHFD1 Human)
UseTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceAug. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLX311-SHOC2-V5
Plasmid#134521PurposeLentiviral plasmid expressing SHOC2 with V5 C-term tagDepositorInsertSHOC2 (SHOC2 Human)
UseLentiviralTagsV5ExpressionMammalianMutationSilent mutations made at wobble base position at …PromoterEF1alphaAvailable sinceSept. 25, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDCAF3-IS5
Plasmid#65220PurposeExpresses N-demethylases for the degredation of caffeine and related methylxanthines. Can be used to measure caffeine content as described in the publication listed below.DepositorInsertsndmA
ndmB
ndmC
ndmD
gst9
chlR
UseSynthetic BiologyTagsExpressionBacterialMutationChanged start codon from gtg to atg and Changed s…PromoterBBa_J23100Available sinceJan. 8, 2016AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAAV_CaMKIIa(0.4)-PdCO-EGFP-WPRE
Plasmid#198514PurposeExpresses optimized PdCO in frame with EGFP under control of minimal CamKIIa promotor.DepositorInsertPdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
A3Bi-ctd E255A -Cas9n-UGI-NLS
Plasmid#109429PurposeExpresses the C-terminal catalytically inactive mutant of human APOBEC3B containing an L1 intron fused to Cas9n, Uracil DNA Glycosylase Inhibitor, with a nuclear localization signal.DepositorInsertApolipoprotein mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal Doman E255A Catalytic Mutant (APOBEC3B Human)
UseCRISPRTagsExpressionMammalianMutationInsertion of an L1 intron into the coding sequenc…PromoterCMVAvailable sinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6sgp53-mTSG-GFAPCre
Plasmid#100276PurposeAAV-CRISPR library for pool mutagenesis of top tumor suppressor genesDepositorUseAAV, CRISPR, Mouse Targeting, and Synthetic Biolo…TagsExpressionMammalianMutationPromoterAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET3a-SF-LMNC-TS
Plasmid#169374PurposeE. coli expression plasmid for human LMNA residues 31-542, corresponding to the shorter lamin C isoform lacking the unstructured head, with an N-terminal splitFlAsH tag and a C-terminal TwinStrep tag.DepositorInsertLMNA residues 31-542 (LMNA Human)
UseTagsSplit-FlAsH Tag and TwinStrep TagExpressionBacterialMutationdeleted amino acid 1-30 (unstructured head domain…PromoterNative pET3a promotorAvailable sinceMay 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-Her1-dL5
Plasmid#73216PurposeBacterial expression vector for Z1907-dL5(E52D) affibody fusion protein (AF) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsert10XHis-GST-HRV-Her1-dL5 (EGFR Schistosoma japonicum, Human, Synthetic)
UseTags10XHis-GST-HRV and The Her1 Affibody and dL5 FAP …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET21-10XHis-GST-HRV-dL5-Her1
Plasmid#73215PurposeBacterial expression vector for dL5(E52D)-Z1907 affibody fusion protein (FA) with N-terminal His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP)DepositorInsertEGFR (EGFR Schistosoma japonicum, Human, Synthetic)
UseTags10XHis-GST-HRV and The dL5 FAP and Her1 affibody …ExpressionBacterialMutationThe dL5** FAP is E50D, L89S, also known as E52D/L…PromoterT7Available sinceMarch 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated
Plasmid#215769Purposeencodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locusDepositorInsertsUseTagsExpressionBacterialMutationCas12a PAM mutatedPromoterAvailable sinceJune 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDH-V1AUC-DelStem-CD20-Puro
Plasmid#209758PurposeLentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1; variant 1 mRNA isoform with mutations to disrupt the uORFs and the stem-loop within the 5'-UTR.DepositorInsertMS4A1 (MS4A1 Human)
UseLentiviralTagsExpressionMutationMutations to disrupt the uORFs and the stem-loop …PromoterAvailable sinceNov. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMIG-hNFATc2/C(1-686, RIT)FLAG-AVITEV
Plasmid#74055Purposeretroviral expression plasmid for human NFATc2/C(1-686, RIT) (without C-terminal region, with RIT mutation abrogating AP1 binding) with C-terminal BirA-biotinylation signal and TEV celavage siteDepositorInserthuman NFATc2, isoform C (NFATC2 AS 1-686 (N terminus deleted), Human)
UseRetroviralTagsAVI-TEV and FLAGExpressionMammalianMutationsilent mutation A1723C in the sequence of NM_1730…PromoterAvailable sinceJune 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CamKIIa(0.4)-stChrimsonR-EGFP-P2A-PdCO-WPRE
Plasmid#202198PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of minimal CamKIIa promotorDepositorInsertstChrimsonR, PdCO
UseAAVTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterCaMKIIα minimal promotor (0.4kb)Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-SARS2-B.1.617.2
Plasmid#172320Purposeexpressing SARS-CoV-2 B.1.617.2 spike protein (delta strain) for pseudovirus production.DepositorInsertSpike of B.1.617.2 strain (S SARS-CoV-2)
UseTagsExpressionMammalianMutationT19R, 156G, 157-158del, L452R, T478K, D614G, P681…PromoterCMV-FAvailable sinceJuly 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE
Plasmid#202199PurposeExpresses bicistronically soma-targeted ChrimsonR in frame with EGFP and the optimized PdCO under control of human synapsin1 promotor after Cre-dependent recombinationDepositorHas ServiceAAV1 and AAV5InsertstChrimsonR, PdCO
UseAAV and Cre/LoxTagsEGFP and Rho1D4ExpressionMammalianMutationPromoterhSyn1Available sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLEX305_FKBP12F36V-SHOC2
Plasmid#134522PurposeLentiviral plasmid expressing SHOC2 with N-term FKBP12(F36V) tagDepositorInsertSHOC2 (SHOC2 Human)
UseLentiviralTagsFKBP12F36V-2xHAExpressionMammalianMutationSilent mutations made at wobble base position at …PromoterhPGKAvailable sinceNov. 21, 2019AvailabilityAcademic Institutions and Nonprofits only