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Plasmid#162297PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0545
Plasmid#162296PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0544
Plasmid#162295PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[Factor Xa cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0543
Plasmid#162294PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0542
Plasmid#162293PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-GST-[thrombin cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0541
Plasmid#162292PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[thrombin cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Integrase triple controller
Plasmid#126540PurposePben-int5; Ptet-bxb1; Pbad-tp901: colE1 Chlo+DepositorInsertBenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2373
Plasmid#160079PurposeZ. mobilis CRISPRi, promoter F, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2371
Plasmid#160078PurposeZ. mobilis CRISPRi, promoter E, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2369
Plasmid#160077PurposeZ. mobilis CRISPRi, promoter D, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2093
Plasmid#160074PurposeZ. mobilis CRISPRi, promoter B, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSWD95
Plasmid#158617PurposeCckA FRET SensorDepositorInsertCckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691, Linker SNVTRHRSAT, mRuby3
UseTagsExpressionBacterialMutationPromoterXyloseAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-09
Plasmid#158437PurposepBAD33 vector encoding MotA, Dendra2 - GSGSGS linker - MotBDepositorInsertsMotA
MotB
UseTagsDendra2- 3 x EAAAK linkerExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-25
Plasmid#158436PurposepBAD33 vector encoding MotA, YPet - 3 x EAAAK linker - MotBDepositorInsertsMotA
MotB
UseTagsyPET - 3 x EAAAK linkerExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMH-05
Plasmid#158435PurposepBAD33 vector encoding MotA, YPet - EAAAK linker - MotBDepositorInsertsMotA
MotB
UseTagsyPET - EAAAK linkerExpressionBacterialMutationPromoterAvailable sinceOct. 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAF302
Plasmid#137752PurposeIntracellular control plasmid for HiBiTopt experimentsDepositorInserthupA-linker-hibitopt
UseTagsHiBiToptExpressionBacterialMutationPromoterPtetAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAP233
Plasmid#137753PurposeExtracellular control for HiBiTopt experimentsDepositorInsertsrtB-linker-hibitopt
UseLuciferaseTagsHiBiToptExpressionBacterialMutationPromoterPtetAvailable sinceSept. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pACYCDuet1-ammA
Plasmid#134682PurposeExpresses His6-AmmA in E. ColiDepositorInsertammA
UseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceJuly 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM587
Plasmid#133593PurposePart Plasmid for dCas9, Part 3b(coding sequence)DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMM586
Plasmid#133592PurposePart Plasmid for dCas9, Part 3(coding sequence)DepositorInsertdCas9
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceMarch 10, 2020AvailabilityAcademic Institutions and Nonprofits only