Addgene

Showing: 3441 - 3460 of 30473 results

Results per page:

Sort results by:

pPD472 CMV MCS (TUPV2)

Plasmid

#139213

Purpose

mMoClo TUPV2, with CMV promoter and an MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pPD471 CMV MCS (TUPV1)

Plasmid

#139212

Purpose

mMoClo TUPV1, with CMV promoter and an MCS

Depositor

Joshua Leonard

Article

Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

Plasmid

#163550

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

RBMX-eA3A-UdgX-HF-NG-nCas9

Use

CRISPR

Tags

Expression

Mammalian

Mutation

nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

Promoter

Available since

Dec. 22, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_RKK/AAA POLI

Plasmid

#131240

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations R524A, K526A and K530A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert arginine re…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_3KA + S580A POLI

Plasmid

#131241

Purpose

Mammalian expression of N-terminally FLAG-tagged DNA polymerase iota containing mutations K440A, K444A, K446A and S580A

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains point mutations that convert Pol iota se…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_1-590 POLI

Plasmid

#131233

Purpose

Mammalian expression of DNA polymerase iota residues 1-590 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV6-AN-DDK_1-420 POLI

Plasmid

#131234

Purpose

Mammalian expression of DNA polymerase iota residues 1-420 with an N-terminal FLAG-tag

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

Insert

DNA polymerase iota (POLI Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Contains a 24 bp insertion within the Pol iota co…

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
p3x-FLAG-CMV-MSTN

Plasmid

#157953

Purpose

expression of MSTN gene

Depositor

Yan-zhen Bi

Insert

MSTN (MSTN Sus scrofa)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pRC/CMV-TYK2VF/PA-VSV

Plasmid

#139351

Purpose

Expression of the double mutant of TYK2-V678F-P1104A

Depositor

Sandra Pellegrini

Article

Li et al J Immunol. 2013 Mar 1;190(5):2335-44. do

Insert

TYK2 cDNA with 267nt of 5' UTR (TYK2 Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

V362F-V678F-P1104A

Promoter

CMV

Available since

June 4, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-ILVBLpA-G10C

Plasmid

#120206

Purpose

Human ILVBL poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

ILVBL poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L4R1-hCMVstar1+bgloInt

Plasmid

#139528

Purpose

Provides conditional promoter cassette between attL4-attR1 on Gateway entry vector

Depositor

Minoru Ko

Insert

hCMVstar1 promoter

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-RPS5pA-del

Plasmid

#120200

Purpose

human RPS5 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-RPS5pA-m2r

Plasmid

#120199

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 2 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-RPS5pA-m1r

Plasmid

#120197

Purpose

Human RPS5 poly(A) site reporter, stem loop mutant 1 rescue

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

RPS5 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-CENPBpA-del

Plasmid

#120193

Purpose

human CENPB poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

CENPB poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-TFE3pA-del

Plasmid

#120204

Purpose

human TFE3 poly(A) site reporter, with stem loop deleted

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-GFP-TFE3pA-G9C

Plasmid

#120202

Purpose

Human TFE3 poly(A) site reporter, stem loop mutant

Depositor

David Bartel

Article

Wu et al Cell. 2017 May 18;169(5):905-917.e11. do

Insert

TFE3 poly(A) site

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 12, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
BII-Sh-PnGW-CMV-Luc

Plasmid

#133367

Purpose

Piggybac vector for shRNA-mediated knockdown, co-expressed with GFP-NLS

Depositor

Blair Madison

Article

Fischer et al Am J Physiol Gastrointest Liver Physiol.

Insert

GFP-NLS

Use

Tags

HA-tagged GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV

Available since

Jan. 3, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-CMV-Flag-hcRed_IDG-K

Plasmid

#135274

Purpose

Gateway destination clone of hcRed (as control) tagged with N-terminal Flag for generating protein-protein networks using fusion tag affinity-based proteomics

Depositor

Ben Major

Insert

Flag-hcRed

Use

Lentiviral; Gateway destination

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV 3xFLAG-ATG3 allKR

Plasmid

#129298

Purpose

Expresses pCMV 3xFLAG-ATG3 allKR (all lysines mutated to arginine) in mammalian cells

Depositor

Robin Ketteler

Article

Agrotis et al J Biol Chem. 2019 Jul 17. pii: AC119.009

Insert

Ubiquitin-like-conjugating enzyme ATG3 (ATG3 Human)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

changed all 22 Lysine residues to Arginine

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge