Addgene

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pGEX Txk-SH2

Plasmid

#46526

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Txk (Txk Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 138-258

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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PRRX2-pSG5

Plasmid

#21009

Depositor

Corey Largman

Article

Stelnicki et al J Invest Dermatol 1998 Jul;111(1):57-63.

Insert

PRRX2 (Prrx2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only

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WT-β3

Plasmid

#166579

Purpose

For expression of cytoplasmic tail of mouse beta3 integrin with N-terminal GST fusion protein in bacterial cells.

Depositor

Vesa Hytönen

Article

Zhang et al Proc Natl Acad Sci U S A. 2020 Dec 22;11

Insert

β3 (Itgb3 Mouse)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

Available since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pRSETB PKA Cat

Plasmid

#14920

Depositor

Susan Taylor

Article

Taylor et al Biochim Biophys Acta. 2005 Dec 30. 1754(

Insert

PKA catalytic subunit alpha (Prkaca Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 24, 2007

Availability

Academic Institutions and Nonprofits only
pBabe(SV40)-Cytochrome C-GFP

Plasmid

#41184

Depositor

Douglas Green

Article

Goldstein et al Nat Cell Biol. 2000 Mar;2(3):156-62.

Insert

Cytochrome C-GFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

SV40

Available since

Nov. 26, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3-myc-mE6-AP/2591a

Plasmid

#11468

Depositor

Allan Weissman

Article

Hatakeyama et al J Biol Chem. 1997 Jun 13. 272(24):15085-

Insert

mE6-AP (Ube3a Mouse)

Use

Tags

myc

Expression

Mammalian

Mutation

n/a

Promoter

Available since

March 8, 2006

Availability

Academic Institutions and Nonprofits only
pB.GFP-KIF1A tail 395-1698

Plasmid

#134618

Purpose

Mammalian expression of mouse KIF1A 395-1698

Depositor

Marvin Bentley

Article

Yang et al Traffic. 2019 Aug 28. doi: 10.1111/tra.1

Insert

KIF1A (Kif1a Mouse)

Use

Tags

eGFP

Expression

Mammalian

Mutation

WT with deletion of the 394 N-terminal residues.

Promoter

Chicken Beta actin

Available since

Jan. 7, 2020

Availability

Academic Institutions and Nonprofits only

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FLAG-MED10

Plasmid

#15417

Depositor

Ronald Conaway, Joan Conaway

Article

Sato et al J Biol Chem. 2003 Apr 25. 278(17):15123-

Insert

MED10 (Med10 Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

N/A

Promoter

Available since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pLV-EF1a-V5-Smpd4

Plasmid

#120237

Purpose

Expresses V5-tagged Smpd4 in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Smpd4 (Smpd4 Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1(-) mouse C/EBP gamma

Plasmid

#12560

Depositor

Peter Johnson

Article

Parkin et al J Biol Chem. 2002 Jun 28;277(26):23563-7

Insert

CCAAT enhancer binding protein gamma (Cebpg Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only
PB-TRE-GNL

Plasmid

#204499

Purpose

Expresses Glis1, Nanog and lin28a by tet-on promoter in mammalian cells

Depositor

Wataru Fujii

Article

Matsuya et al Sci Rep. 2024 Feb 8;14(1):3204. doi: 10.

Insert

Glis1 (Glis1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pBS CRX (CC#4)

Plasmid

#15127

Depositor

Connie Cepko

Article

Furukawa et al Cell. 1997 Nov 14. 91(4):531-41.

Insert

CRX (Crx Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 8, 2007

Availability

Academic Institutions and Nonprofits only
pGEN-mSmoA1

Plasmid

#37674

Depositor

Philip Beachy

Article

Taipale et al Nature. 2000 Aug 31;406(6799):1005-9.

Insert

Smo (Smo Mouse)

Use

Tags

Expression

Mammalian

Mutation

W539L

Promoter

CMV

Available since

Nov. 12, 2012

Availability

Academic Institutions and Nonprofits only

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Bmi1 shRNA1

Plasmid

#21576

Depositor

Sally Temple

Article

Fasano et al Cell Stem Cell. 2007 Jun 7. 1(1):87-99.

Insert

Bmi-1 (Bmi1 Mouse)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 2, 2010

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-mLepr-Fc(DAPA)-AviTag-6xHis

Plasmid

#157617

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

Lepr (Lepr Mouse)

Use

Tags

Fc(DAPA)-Avi-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti-CRISPR-v2-gYap-1

Plasmid

#166481

Purpose

Expresses spCas9 and a gRNA targeting mouse Yap

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

gRNA for mouse Yap (Yap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 13, 2021

Availability

Academic Institutions and Nonprofits only

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Venus Cam KII alpha

Plasmid

#29428

Depositor

Steven Vogel

Article

Thaler et al Proc Natl Acad Sci U S A. 2009 Apr 14. 1

Insert

Venus CAM KII Alpha

Use

Tags

Venus

Expression

Mammalian

Mutation

Promoter

Available since

June 3, 2011

Availability

Academic Institutions and Nonprofits only
PLEXIN A4

Plasmid

#58239

Purpose

Riboprobe for in vitro transcription of Plexin A4

Depositor

Christiana Ruhrberg

Article

Schwarz et al Dev Biol. 2008 Dec 1;324(1):1-9. doi: 10

Insert

Plexin A4 (Plxna4 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only
Lenti-sgKmt2d#1/Cre

Plasmid

#173597

Purpose

Expresses a Kmt2d-targeting gRNA and Cre-recombinase

Depositor

Charles Swanton

Article

Cai et al Cancer Discov. 2021 Feb 19. pii: 2159-82

Insert

gRNA targeting Kmt2d (Kmt2d Mouse)

Use

CRISPR, Cre/Lox, and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

May 12, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA-Flag-Kdm6b(1025-End)_H1390A-pA

Plasmid

#100279

Purpose

In vitro transcription template (T7 promoter) for Kdm6b catalytic domain mutant

Depositor

Yi Zhang

Article

Inoue et al Nature. 2017 Jul 19. doi: 10.1038/nature

Insert

Kdm6b (Kdm6b Mouse)

Use

Tags

Flag epitope tag

Expression

Mammalian

Mutation

Mutated catalytic domain (1025aa-End) (please see…

Promoter

Available since

Sept. 25, 2017

Availability

Academic Institutions and Nonprofits only

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