Addgene

We narrowed to 3,798 results for: SPL

Showing: 3441 - 3460 of 3798 results

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pTag4C_ADRA1A-V2R-NTEV-tcs-GV

Plasmid

#227101

Purpose

ADRA1A receptor for split TEV assay

Depositor

Michael Wehr

Insert

ADRA1A-NTEV (ADRA1A Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pJM154

Plasmid

#218121

Purpose

Hybrid circuit displaying FKBP12 bait and driving expression of luxAB

Depositor

David Liu

Article

Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

Inserts

FKBP12
luxAB

Use

Luciferase

Tags

434cI_RR69

Expression

Mutation

Promoter

Available since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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AA265

Plasmid

#215967

Purpose

Fragmid fragment: (Pol 2 promoter) spleen focus-forming virus promoter

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Has Service

Cloning Grade DNA

Insert

SFFV_v1

Use

Fragment

Tags

Expression

Mutation

Promoter

Available since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJET-LB-geneticin

Plasmid

#213468

Purpose

Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

Depositor

Kim Hammond-Kosack

Article

Darino et al Fungal Biol Biotechnol. 2024 Feb 26;11(1

Insert

Left border of the TSI locus 1-split fragment of geneticin cassette

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

April 24, 2024

Availability

Academic Institutions and Nonprofits only

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p4A8_M59I_T94M_BC

Plasmid

#217833

Purpose

4A8 variant in barcoded, yeast surface display plasmid

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

4A8 variant Fab

Use

Tags

Expression

Yeast

Mutation

VH: M59I, VL: T94M

Promoter

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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FLAG NC

Plasmid

#214675

Purpose

Negative control plasmid for splicing reporters consisting of MCP fused FLAG epitope tags

Depositor

Eugene Yeo

Article

Schmok et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

FLAG

Use

Tags

MCP and V5

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3_ERBB4-NTEV-tcs-GV-2xHA

Plasmid

#214613

Purpose

ERBB4 receptor for split TEV assay

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

ERBB4-NTEV (ERBB4 Human)

Use

Tags

TEV-Gal4-VP16-2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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185_pETcon_SARS2_BQ1-1

Plasmid

#208085

Purpose

yeast surface display of the SARS-CoV-2 BQ.1.1 variant RBD

Depositor

Tyler Starr

Article

Taylor et al PLoS Pathog. 2023 Dec 29;19(12):e1011901

Insert

SARS-CoV-2 BQ.1.1 RBD (S Budding Yeast, SARS-CoV-2 virus)

Use

Tags

HA and c-Myc

Expression

Yeast

Mutation

Promoter

Available since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLV-SFFV-Llgl1-WPRE-UbC-Emerald

Plasmid

#203805

Purpose

Lentiviral vector plasmid expressing mouse Llgl1 under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

Depositor

Bart Nieuwenhuis, Richard Eva

Inserts

Llgl1 (Llgl1 Mouse)
Emerald

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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mCh-Miro1(∆TM)-SspB

Plasmid

#194007

Purpose

Optogenetic tool

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Miro1 (var4, peroxisomal splice variant) (RHOT1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo W42A

Plasmid

#191399

Purpose

W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo W42A

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

W42A

Promoter

CMV

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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EK0362 SFFV-mCherry-SG(Bdnf.s1L36+54@202)SG-EGFP (FLP-IN)

Plasmid

#191153

Purpose

Mammalian expression of a 90 bp (36+54) long sensor for murine Bdnf 3' UTR where the trigger sequence is split

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:Bdnf.s1L36+54:EGFP

Use

Tags

Expression

Mammalian

Mutation

WT

Promoter

SFFV

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pSEM264 - pDESTR4-R3 mosTI Pmlc-2_GFP target

Plasmid

#159842

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pDESTR4-R3 mosTI Pmlc-2_GFP target

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Use

Tags

Cmyc and HA

Expression

Yeast

Mutation

Promoter

Available since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pKB238

Plasmid

#170033

Purpose

Encodes LacI-controlled expression of surface-displayed NPY and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
Neuropeptide Y (NPY Human, Escherichia coli, Synthetic)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pKB235

Plasmid

#170024

Purpose

Encodes LacI-controlled expression of surface-displayed CCL20 and PvirK-mNeonGreen reporter

Depositor

Jeffrey Tabor

Article

Brink et al bioRxiv

Inserts

mNeonGreen
CCL20 (CCL20 Human, Escherichia coli, Synthetic)

Use

Synthetic Biology

Tags

Lpp, OmpA, and Tether

Expression

Bacterial

Mutation

Promoter

Ptac and PvirK

Available since

July 1, 2021

Availability

Academic Institutions and Nonprofits only

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pSEM323 - array co-injection fragment mosTI Pmlc-2_GFP

Plasmid

#159836

Purpose

Single copy or array insertion by MosTI using split Pmlc-2 GFP selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

array co-injection fragment mosTI Pmlc-2_GFP

Use

Tags

Expression

Worm

Mutation

Not applicable

Promoter

Available since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_018

Plasmid

#123733

Purpose

Encodes rapamycin and tamoxifen activated split-dCAS9 (N-terminal) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

spdCas9(1-204)-frb-D10A-ERT2

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMT dati MCS Exon

Plasmid

#110119

Purpose

Expression plasmid for expressing circular RNAs of a desired sequence in Drosophila using the dati flanking introns to drive backsplicing

Depositor

Jeremy Wilusz

Article

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

dati (dati Fly)

Use

Tags

Expression

Insect

Mutation

Promoter

Metallothionein Promoter (pMT)

Available since

June 1, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mus-Bnip3_deltaExon3

Plasmid

#100793

Purpose

Mammalian expression of Bnip3_deltaExon3 splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mouse-Bnip3_deltaExon3 (Bnip3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Exon3 deletion

Promoter

CMV

Available since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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