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pR2Q024y

Plasmid

#101022

Purpose

fluorescent, functional, glutamate receptor subunit

Depositor

Thomas Hughes

Article

Sheridan et al Neuroscience. 2006 Aug 25;141(2):837-49.

Insert

Glutamate receptor subunit (Gria2 Rat)

Use

Tags

fluorescent protein

Expression

Mammalian

Mutation

tagged with fluorescent protein

Promoter

cmV

Available since

July 27, 2018

Availability

Academic Institutions and Nonprofits only

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SLF063: Lenti-fCAG-jRGECO1a

Plasmid

#124775

Purpose

For general lentiviral or direct expression of cytosolic jRGECO1a

Depositor

Adam Cohen

Article

Farhi et al J Neurosci. 2019 Apr 5. pii: JNEUROSCI.0

Insert

jRGECO1a

Use

Lentiviral

Tags

NES-6xHIS

Expression

Mammalian

Mutation

Promoter

CAG

Available since

June 4, 2019

Availability

Academic Institutions and Nonprofits only
pNIR-FbGFP

Plasmid

#184675

Purpose

NIR-Fb to GFP

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 May 23. pii: 10.1038/s

Insert

NIR-FbGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 23, 2022

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-5ptaseOCRL

Plasmid

#66836

Purpose

Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

5ptaseOCRL (OCRL Human)

Use

Tags

mCherry-CRY2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pBad33_ISYmu1_reRNA

Plasmid

#205358

Purpose

Bacterial expression of ISYmu1 TnpB and its reRNA

Depositor

Haoyi Wang

Article

Xiang et al Nat Biotechnol. 2023 Jun 29. doi: 10.103

Insert

ISYmu1 TnpB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pBID-UASC

Plasmid

#35200

Depositor

Brian McCabe

Article

Wang et al PLoS One. 2012;7(7):e42102. Epub 2012 Ju

Type

Empty backbone

Use

Drosophila transgene expression

Tags

Expression

Mutation

Promoter

Drosophila Synthetic Core Promoter (DSCP)

Available since

July 26, 2012

Availability

Academic Institutions and Nonprofits only

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Arf1-GFP

Plasmid

#39554

Depositor

Paul Melancon

Article

Chun et al Mol Biol Cell. 2008 Aug;19(8):3488-500.

Insert

ADP ribosylation factor 1 (ARF1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 10, 2012

Availability

Academic Institutions and Nonprofits only

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LV-TRE-VP64 mouse MyoD-T2A-dsRedExpress2

Plasmid

#60625

Purpose

Expresses VP64 mouse MyoD and dsRedExpress2 in response to doxycycline

Depositor

Charles Gersbach

Article

Kabadi et al ACS Synth Biol. 2014 Dec 10.

Insert

VP64 mouse MyoD

Use

Lentiviral

Tags

Expression

Mutation

Promoter

TRE

Available since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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TRUPATH Triple (bidirectional) GasS

Plasmid

#196055

Purpose

Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

Depositor

Justin English

Article

Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

Inserts

GAlphasS-RLuc8
GGamma9-GFP2
GBeta3 (GNB3 Human)

Use

Luciferase

Tags

GFP2 and GSAG linker

Expression

Mammalian

Mutation

RLuc8 and flanking SGGGGS linkers have been inser…

Promoter

CMV

Available since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pATetO 6XHis-Taq DNA Ligase

Plasmid

#178798

Purpose

Inducible expression of Taq DNA Ligase in E. coli

Depositor

Andrew Ellington

Article

Bhadra et al PLoS One. 2021 Jun 1;16(6):e0252507. doi

Insert

Taq DNA Ligase

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +2

Plasmid

#66941

Purpose

CRISPaint frame selector +2

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +2

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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ecAT3.10

Plasmid

#107215

Purpose

Mammalian expression plasmid to target the ecAT3.10 extracellular ATP sensor to the cell surface.

Depositor

Mathew Tantama

Article

Conley et al PLoS One. 2017 Nov 9;12(11):e0187481. do

Insert

ecAT3.10

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pBPGAL4.2Uw-2

Plasmid

#26227

Depositor

Gerald Rubin

Article

Pfeiffer et al Genetics. 2010 Aug 9. ():.

Insert

GAL4 (GAL4 Budding Yeast)

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Oct. 7, 2010

Availability

Academic Institutions and Nonprofits only

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pR26 GFP Dest

Plasmid

#74283

Purpose

Gene targeting vector for the mouse Rosa26 locus, including a loxP flanked stop cassette and GFP, for gateway cloning

Depositor

Ralf Kuehn

Article

Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

Insert

Rosa26 5-homology region

Use

Tags

Expression

Mutation

Promoter

Available since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pHR_Gal4UAS_IRES_mC_pGK_tBFP

Plasmid

#79123

Purpose

Lentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors

Depositor

Wendell Lim

Article

Morsut et al Cell. 2016 Feb 11;164(4):780-91. doi: 10

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCAS9-mCherry-Frame +0

Plasmid

#66939

Purpose

CRISPaint frame selector +0

Depositor

Veit Hornung

Article

Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

Insert

gRNA frame +0

Use

Tags

Expression

Mutation

Promoter

Available since

Sept. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2-FUS-1-526aa-V5

Plasmid

#29609

Depositor

Aaron Gitler

Article

Sun et al PLoS Biol. 2011 Apr;9(4):e1000614. doi:

Insert

FUS (FUS Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3_FLAG_MTA3

Plasmid

#31696

Depositor

Paul Wade

Article

Fujita et al Cell. 2003 Apr 18. 113(2):207-19.

Insert

Metastasis associated protein 3 (MTA3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)/AUG-nLuc-3XFLAG

Plasmid

#127299

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 21, 2019

Availability

Academic Institutions and Nonprofits only

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pLD037-pCMV-APOBEC-Cas9(D10A)-rXRCC1

Plasmid

#165444

Purpose

Expresses ACX, rXRCC1 in mammalian cells

Depositor

Wei Leong Chew

Article

Chen et al Nat Commun. 2021 Mar 2;12(1):1384. doi:

Insert

APOBEC-nCas9-rXRCC1 (Apobec1 Synthetic, Streptococcus pyogenes, Rat)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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