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Plasmid#164215PurposepLEX lentivirus backbone expresses EYFP tagged LAMP1 under PGK promoterDepositorInsertLAMP1 (Lamp1 Rat)
UseLentiviralTagsEYFPExpressionMutationPromoterPGKAvailable sinceMarch 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCSC-NLS-EGFP
Plasmid#221811PurposeLentiviral vector expressing green fluorescent protein (GFP) fused with nuclear localization sequence (NLS)DepositorInsertNLS-EGFP
UseLentiviralTagsNLSExpressionMammalianMutationPromoterCMVAvailable sinceJuly 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
gCH27 (crCD55-4)
Plasmid#217343PurposecrRNA targeting CD55DepositorInsertcrCD55-4 (CD55 Human)
UseCRISPR and LentiviralTagsExpressionMutationPromoterhU6Available sinceMarch 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
TFORF2297
Plasmid#143361PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertTAF10 (TAF10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
ICP8-P2A/Crimson (pSLIK4)
Plasmid#113859PurposeFor Doxycycline-inducible expression of ICP8 synaptase gene, with ICP8-P2A-red fluorescent protein gene E2-CrimsonDepositorInsertICP8
UseLentiviralTagsP2A/CrimsonExpressionMutationPromoterAvailable sinceJune 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-mScarlet-I
Plasmid#159172PurposeTo express a bright monomeric red FP for labelling and detection of mammalian cellsDepositorInsertmScarlet-I
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationCodons were optimized to Human codon usagePromoterCMVAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
Lenti-DIO-P-EF1a-RPL22-3xHA-IRES-YFP
Plasmid#170327PurposeDouble-floxed inverse Orientation HA and IRES-YFP tagged RPL22DepositorInsertRPL22 (RPL22 Human)
UseLentiviralTagsExpressionMutationNonePromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Fuw-dCas9-Dnmt3a_IM
Plasmid#84478PurposeLentiviral construct to express dCas9 fused with an inactive Dnmt3a catalytic domain (E664A, E756A)DepositorInsertdCas9-Dnmt3a_IM
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
lentiCRISPRi(v1)-Blast
Plasmid#170067PurposeExpresses dCas9 repressor KRAB-dCas9 and blasticidin resistanceDepositorInsertKRAB-dCas9
UseCRISPR and LentiviralTags2A and 2xHA tagExpressionMammalianMutationPromoterEFS-NSAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
EF1a_MYOD1_P2A_Hygro_Barcode
Plasmid#120464PurposeBarcoded lentiviral vector to express MYOD1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorInsertMYOD1 (MYOD1 Human)
UseLentiviralTagsExpressionMutationPromoterEF1aAvailable sinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLV[Exp]-EF1A>Tet3G:IRES:Neo
Plasmid#198756PurposeVector encodes a Tet-On Advanced transactivator under control of a constitutive EF1α promoter and confers resistance to the antibiotic G418DepositorInsertTet3G, IRES, Neo
UseLentiviralTagsExpressionMutationPromoterEF1AAvailable sinceJuly 31, 2023AvailabilityAcademic Institutions and Nonprofits only -
EF.hICN1.Ubc.GFP
Plasmid#17626DepositorInsertICN1 (NOTCH1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
TFORF2659
Plasmid#144215PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertSTAT2 (STAT2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2402
Plasmid#142413PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertNFE2L2 (NFE2L2 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE
Plasmid#139094PurposeExpresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.DepositorInsertKRAB-dSpCas9
UseCRISPR and LentiviralTagsExpressionMammalianMutationD10A, H840APromoterAvailable sinceMarch 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pXL004-ishRNA-scramble
Plasmid#36311DepositorInsertshRNA of scramble
UseLentiviral and RNAiTagsEF1a-TetR-IRES-RFP-PuroExpressionMammalianMutationPromoterTetO-H1Available sinceJuly 9, 2012AvailabilityAcademic Institutions and Nonprofits only -
pLminP_Luc2P_RE23
Plasmid#90365PurposeATF4 - Amino acid deprivation gene reporterDepositorInsertFirefly luciferase
UseLentiviralTagsExpressionMutationPromoterAAREAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLKO-puro-tetON-shRFP
Plasmid#110940PurposeTet-inducible shRNA targeting RFPDepositorInsertshRFP
UseLentiviral and RNAiTagsExpressionMutationPromoterH1/TOAvailable sinceNov. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVX-dL5**-mCER-TRF1
Plasmid#168176PurposeExpresses FAP (dL5**) fused to mCerulean and the telomere binding protein TRF1DepositorInsertTRF1 (TERF1 Human)
UseLentiviralTagsFAP (dL5**) and mCeruleanExpressionMammalianMutationPromoterAvailable sinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCDF1-PTPRD-WT
Plasmid#25642DepositorInsertProtein tyrosine phosphatase receptor-type delta (PTPRD Human)
UseLentiviralTagsExpressionMammalianMutationAn NsiI restriction site was engineered into the …PromoterAvailable sinceApril 21, 2011AvailabilityAcademic Institutions and Nonprofits only