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AICSDP-078:G3BP1-mEGFP

Plasmid

#193920

Purpose

Homology arms and mEGFP-SGLRS for N-terminus tagging of human G3BP stress granule assembly factor 1

Depositor

Allen Institute for Cell Science

Insert

G3BP1 Homology Arms with mEGFP-SGLRS (G3BP1 Human)

Use

CRISPR; Donor template

Tags

mEGFP-linker

Expression

Mutation

homology arms contain point mutations to disrupt …

Promoter

Available since

Dec. 22, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pNL4-3 envFS eGFP NoStarts

Plasmid

#101342

Purpose

All ATGs from the start of gag to NC mutated to ATC except the first which was mutated to ACG

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

No methonines in Gag

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-LacI-NLS-VP16

Plasmid

#103836

Purpose

Transcription activator (transactivation domain of viral VP16 protein) that is constitutively recruited lacO arrays

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

EGFP-LacI-NLS-VP16

Use

Tags

Expression

Mammalian

Mutation

Promoter

SV2

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-GFP-SF3B6-FLAG

Plasmid

#86870

Purpose

To express SF3B6 with a GFP and FLAG tags at N- and C-terminus, respectively.

Depositor

Yihong Ye

Article

Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

Insert

SF3B6 (SF3B6 Human)

Use

Tags

EGFP and FLAG

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

April 5, 2017

Availability

Academic Institutions and Nonprofits only

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pCXLE-dCas9VP192-T2A-EGFP

Plasmid

#69536

Purpose

Episomal plasmid encoding dCas9VP192

Depositor

Timo Otonkoski

Article

Balboa et al Stem Cell Reports. 2015 Sep 8;5(3):448-5

Insert

dCas9-dCas9VP192-EGFP

Use

CRISPR

Tags

EGFP

Expression

Mutation

Promoter

CAG

Available since

Nov. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pPB-MGT1-d2EGFP_CMV-mCherry

Plasmid

#213763

Purpose

Fluorescent reporter for genetic tracing of mesenchymal Glioblastoma cell-state.

Depositor

Gaetano Gargiulo

Article

Company et al Nat Commun. 2024 Feb 5;15(1):897. doi: 1

Insert

MGT#1-d2EGFP

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pZac2.1 gfaABC1D-EGFP-Kir4.1

Plasmid

#52874

Purpose

Express EGFP-Kir4.1 fusion protein in astrocytes

Depositor

Baljit Khakh

Article

Tong et al Nat Neurosci. 2014 May;17(5):694-703. do

Insert

Kir4.1 (Kcnj10 Rat)

Use

AAV; Adeno-associated virus ()

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 5, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV2_hSyn_NES-Caprola_06-mEGFP_WRPE-SV40

Plasmid

#194690

Purpose

hSyn1 driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through AAV transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

AAV

Tags

mEGFP

Expression

Mutation

Promoter

hSyn1

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET22b-mEB2-eGFP-6xHis

Plasmid

#130982

Purpose

Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB2-GFP-6xHis (Mapre2 Mouse)

Use

Tags

eGFP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pNESx2-EGFP-C1-PABDx2-Spo20

Plasmid

#220082

Purpose

PA biosensor with tandem Spo20-PABDs and two NES domains to aid in nuclear export

Depositor

Gerry Hammond

Article

Weckerly et al bioRxiv [Preprint]. 2024 Feb 28:2024.02.

Insert

Spo20 (SPO20 Budding Yeast)

Use

Tags

X. leavis map2k1.L(32-44)-GGSG-X. leavis map2k1.L…

Expression

Mammalian

Mutation

amino acids 51-91, tandem dimer

Promoter

CMV

Available since

May 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-eGFP-NLS-RNF168

Plasmid

#133977

Purpose

mammalian expression vector of eGFP tagged wild type RNF168

Depositor

Daniel Durocher

Article

Stewart et al Cell. 2009 Feb 6;136(3):420-34. doi: 10.

Insert

RNF168 (RNF168 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

siRNA resistant sequence: GAGGAGTCGTGTTTATTGA

Promoter

CMV

Available since

Nov. 4, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pTRE3G-EGFPC-uL10-(Neo)R

Plasmid

#158069

Purpose

eGFP and uL10 ribosomal protein under the TRE3G promoter, Neo resistance

Depositor

Adolfo Rivero-Muller

Article

Michalec-Wawiorka et al Int J Mol Sci. 2021 May 14;22(10). pii:

Insert

RPLP0 (RPLP0 Human)

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

TRE3G

Available since

Nov. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO_EGFP-RandE3

Plasmid

#79872

Purpose

For generating adeno-associated virus to express RandE3 under the EF1a promoter in a 'Cre-on' dependent manner.

Depositor

Don Arnold

Article

Gross et al Nat Methods. 2016 Jun 6. doi: 10.1038/nm

Insert

GFP-RandE3

Use

AAV, Cre/Lox, and Synthetic Biology

Tags

GFP and HA

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

July 21, 2016

Availability

Academic Institutions and Nonprofits only

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IRE1 alpha KA-pcDNA3.EGFP

Plasmid

#13010

Depositor

Fumihiko Urano

Article

Lipson et al Cell Metab. 2006 Sep . 4(3):245-54.

Insert

Inositol Requiring Enzyme-1 (ERN1 Human)

Use

Tags

Expression

Mammalian

Mutation

R4L, K599A

Promoter

CMV

Available since

Nov. 27, 2006

Availability

Academic Institutions and Nonprofits only

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pCMMP-LMP1-IRES-eGFP

Plasmid

#36955

Depositor

Bill Sugden

Article

Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

Insert

LMP1 (LMP-1 )

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 10, 2012

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Blast-rLAMP1-eGFP

Plasmid

#109028

Purpose

stable lentiviral expression of cDNA

Depositor

Jacob Corn

Article

Liang et al J Cell Biol. 2018 Aug 24. pii: jcb.20180

Insert

rLAMP1

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

EIF-1a promoter

Available since

Aug. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP - FLAG-APEX2- hs Lamin B1

Plasmid

#139442

Purpose

APEX2-Lamin B1 expression plasmid

Depositor

Yixian Zheng

Article

Tran et al bioRxiv 2020.02.05.935635

Insert

LMNB1 (LMNB1 Human)

Use

Tags

3xGGGGS linker (between APEX2 and Lamin B1), APEX…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 13, 2020

Availability

Academic Institutions and Nonprofits only

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EGFP-Talin head-SpyCatcher003

Plasmid

#133566

Purpose

Expresses EGFP-Talin head-SpyCatcher003 in mammalian cells

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

EGFP-Talin head-SpyCatcher003

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pCAGGS-Stargazin-mEGFP-iLID

Plasmid

#178523

Purpose

Expresses Stargazin-mEGFP-iLID in mammalian cells

Depositor

Kazuhiro Aoki

Article

Yamamoto et al Nat Commun. 2021 Dec 8;12(1):7145. doi:

Insert

Stargazin-mEGFP-iLID

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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