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PurposeExpress EGFP-Kir4.1 fusion protein in astrocytes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 52874 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepZac 2.1
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Vector typeMammalian Expression, AAV ; Adeno-Associated Virus ()
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameKir4.1
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Alt nameKcnj10
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SpeciesR. norvegicus (rat)
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GenBank IDNM_031602
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Entrez GeneKcnj10
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer T7
- 3′ sequencing primer EBV-rev (Common Sequencing Primers)
Resource Information
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pZac2.1 gfaABC1D-EGFP-Kir4.1 was a gift from Baljit Khakh (Addgene plasmid # 52874 ; http://n2t.net/addgene:52874 ; RRID:Addgene_52874) -
For your References section:
Astrocyte Kir4.1 ion channel deficits contribute to neuronal dysfunction in Huntington's disease model mice. Tong X, Ao Y, Faas GC, Nwaobi SE, Xu J, Haustein MD, Anderson MA, Mody I, Olsen ML, Sofroniew MV, Khakh BS. Nat Neurosci. 2014 May;17(5):694-703. doi: 10.1038/nn.3691. Epub 2014 Mar 30. 10.1038/nn.3691 PubMed 24686787