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Plasmid#104150PurposedCas9 plasmid expressing full length mKate2 mRNA without RBSDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only
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PAJ389
Plasmid#104147PurposedCas9 plasmid expressing 2A sRNA triggerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
PAJ390
Plasmid#104148PurposedCas9 plasmid expressing 3A sRNA triggerDepositorTypeEmpty backboneUseCRISPRTagsExpressionBacterialMutationPromoterpLtetO-1Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEM-Cas9HF1-D1135E
Plasmid#154924PurposeaTc inducible Cas9HF1 with D1135E mutationDepositorInsertCas9HF1-D1135E
UseCRISPR and Synthetic BiologyTagsssrAExpressionBacterialMutationN497A/R661A/Q695A/Q926A/D1135E and stop codon on …PromoterpTetAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0027
Plasmid#162309PurposeLevel 0 C-terminal tag part for MoClo assembly, TTCG-GCTTDepositorInsertC-terminal tag, 9 aa linker, superfolder GFP, strep tag, stop codon
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0548
Plasmid#162299PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-TrxA-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0550
Plasmid#162301PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-SUMO-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEE22
Plasmid#161787PurposeHi-SC2 reporter delivery plasmidDepositorInsertHi-SC2 reporter
UseConjugative plasmid to deliver hi-sc2 reporterTagsExpressionMutationHimar site included MmeI site, tandem loxP sites,…Promotern/aAvailable sinceDec. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0539
Plasmid#162306PurposeLevel 0 CDS part for MoClo assembly, AATG-TTCGDepositorInsertCDS, TEV protease (S219V), no stop codon
UseSynthetic BiologyTagsExpressionMutationS219V, codon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0551
Plasmid#162303PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-[S-tag]-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0547
Plasmid#162298PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, TrxA-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0546
Plasmid#162297PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0545
Plasmid#162296PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-MBP-[Factor Xa cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0544
Plasmid#162295PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, MBP-[Factor Xa cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0543
Plasmid#162294PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[TEV cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0542
Plasmid#162293PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, HiBiT-GST-[thrombin cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0541
Plasmid#162292PurposeLevel 0 N-terminal tag part for MoClo assembly, CCAT-AATGDepositorInsertN-terminal tag, GST-[thrombin cleavage site]
UseSynthetic BiologyTagsExpressionMutationcodon optimized for E. coliPromoterAvailable sinceDec. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
Integrase triple controller
Plasmid#126540PurposePben-int5; Ptet-bxb1; Pbad-tp901: colE1 Chlo+DepositorInsertBenR_pBEN-int5_pTet-bxb1_AraC_pBAD-tp901-1
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2373
Plasmid#160079PurposeZ. mobilis CRISPRi, promoter F, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJMP2371
Plasmid#160078PurposeZ. mobilis CRISPRi, promoter E, empty guide, sfGFPDepositorInsertempty sgRNA
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 17, 2020AvailabilityAcademic Institutions and Nonprofits only