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GFP-BLM DM

Plasmid

#80071

Purpose

Expression of a sumo-mutant form GFP-BLM with K317R and K331R sumo-site mutations

Depositor

Nathan Ellis

Article

Eladad et al Hum Mol Genet. 2005 May 15;14(10):1351-6

Insert

BLM (BLM Human)

Use

Tags

GFP-BLM

Expression

Mammalian

Mutation

K317R (AAA>AGA) and K331R (AAA>AGA)

Promoter

Available since

July 25, 2016

Availability

Academic Institutions and Nonprofits only

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GFP-SBP-VAP-A*

Plasmid

#120175

Purpose

Expresses a chimera of GFP (fluorescent tag), SBP tag and the C-terminal of ER-resident protein VAP-A

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

VAMP associated protein A (VAPA Human)

Use

Tags

EGFP and SBP

Expression

Mammalian

Mutation

Truncated VAP-A: 118-249

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-hTRF2-tagRFP-T

Plasmid

#103812

Purpose

BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN-hTRF2-tagRFP-T (CIB1, TERF2 Human, Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

hTRF2: deletion of amino acids 1-42 and 476 compa…

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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mCh-KIF13A*-strep

Plasmid

#120166

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF13A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 13A (KIF13A Human)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF13A: 1-639 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_ARHGAP4-1/1

Plasmid

#91518

Purpose

Protein expression and purification of human SH3 domain construct ARHGAP4-1/1

Depositor

Sachdev Sidhu

Article

Teyra et al Structure. 2017 Oct 3;25(10):1598-1610.e

Insert

ARHGAP4-1/1 (ARHGAP4 Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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mApple-M11-92

Plasmid

#134466

Purpose

M11-92 expression

Depositor

Jennifer Lippincott-Schwartz

Article

Chang et al J Cell Biol. 2019 Aug 5;218(8):2583-2599

Insert

M1 Spastin Amino Acids 1-92 (SPAST Human)

Use

Tags

Expression

Mammalian

Mutation

M1 Spastin Amino Acids 1-92

Promoter

CMV

Available since

Nov. 25, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-TPD54(R159E)

Plasmid

#172453

Purpose

Expression of Tumor Protein D52 like 2 (TPD54/TPD52L2) with N-terminal mCherry-FKBP tag R159E mutant

Depositor

Stephen Royle

Article

Larocque et al J Cell Biol. 2021 Oct 4;220(10). pii: 21

Insert

TPD54 (TPD52L2 Human)

Use

Tags

mCherry-FKBP

Expression

Mammalian

Mutation

changed Arginine 159 to Glutamic Acid

Promoter

CMV

Available since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV NucleoBAS N354K-I372V

Plasmid

#63574

Purpose

Fluorescent bile acid sensor (BAS) targeted to nucleus that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET

Depositor

Stan van de Graaf

Article

van der Velden et al Hepatology. 2013 Feb;57(2):740-52. doi:

Insert

NucleoBAS N354K-I372V

Use

Tags

SV40 NLS

Expression

Mammalian

Mutation

N345K and I372V

Promoter

CMV

Available since

May 11, 2015

Availability

Academic Institutions and Nonprofits only
FusionRed-Dectin1A-C-10

Plasmid

#56111

Purpose

Localization: Membrane, Excitation: 580, Emission: 608

Depositor

Michael Davidson

Article

Shemiakina et al Nat Commun. 2012;3:1204. doi: 10.1038/nc

Insert

Dectin1A (CLEC7A Human)

Use

Tags

FusionRed

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 1, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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YFP-Parkin C431N

Plasmid

#46924

Purpose

expresses mutant Parkin with YFP tag in mammalian cells

Depositor

Richard Youle

Article

Lazarou et al J Cell Biol. 2013 Jan 21;200(2):163-72.

Insert

Park2 (PRKN Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed cysteine 431 to asparagine

Promoter

CMV

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEGFP-hGATE-16

Plasmid

#87870

Purpose

Express a EGFP-human GATE-16 in mammalian cells

Depositor

Isei Tanida, Eiki Kominami

Article

Tanida et al J Biol Chem. 2001 Jan 19;276(3):1701-6.

Insert

GATE-16 (GABARAPL2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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mCherry-YOD1

Plasmid

#85663

Purpose

Expression of human YOD1 with N-terminal mCherry tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-YOD1

Plasmid

#85664

Purpose

Expression of human YOD1 with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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FRB-GFP-Gab2(Y604F/Y634F)

Plasmid

#188659

Purpose

FRB coupled to GFP tagged Gab2 with Shp2 binding sites mutated to Phe

Depositor

Andrei Karginov

Article

Fauser et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

GRB associated binding protein 2 (Gab2 Mouse)

Use

Tags

FRB and GFP

Expression

Mammalian

Mutation

Y604F and Y634F

Promoter

CMV

Available since

Aug. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP VAMP7 (102-220)

Plasmid

#42314

Depositor

Thierry Galli

Article

Martinez-Arca et al J Cell Biol. 2000 May 15;149(4):889-900.

Insert

VAMP7 Coiled Coil domain (102-220) (VAMP7 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Δ1-102

Promoter

CMV

Available since

March 13, 2013

Availability

Academic Institutions and Nonprofits only
mCardinal-ZO1-C-14

Plasmid

#56179

Purpose

Localization: Tight Junctions, Excitation: 604, Emission: 659

Depositor

Michael Davidson

Article

Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

Insert

ZO1 (TJP1 Human)

Use

Tags

mCardinal

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mCh-KIF5A*-strep

Plasmid

#120163

Purpose

Expresses a chimera of mCherry (fluorescent tag), a KIF5A-based anterograde motor and streptavidin

Depositor

Juan Bonifacino

Article

Guardia et al PLoS Biol. 2019 May 17;17(5):e3000279. d

Insert

kinesin family member 5A (Kif5a Mouse)

Use

Tags

Streptavidin and mCherry

Expression

Mammalian

Mutation

Truncated KIF5A: 1-559 aa

Promoter

CMV

Available since

July 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEGFP-YOD1-C160S

Plasmid

#85666

Purpose

Expression of human YOD1 C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C160S

Promoter

CMV

Available since

Feb. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-TDAG51

Plasmid

#32699

Depositor

Richard C. Austin

Article

Hossain et al J Biol Chem. 2003 Aug 8;278(32):30317-27

Insert

human tdag51 (PHLDA1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 14, 2013

Availability

Academic Institutions and Nonprofits only
KCC2-pHext

Plasmid

#104076

Purpose

To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

Depositor

Igor Medyna

Article

Friedel et al eNeuro. 2017 Jul 21;4(4). pii: ENEURO.00

Insert

KCC2 (b isoform) (Slc12a5 Rat)

Use

Tags

pHluorine tag inserted in the second putative tra…

Expression

Mammalian

Mutation

EcoR1 site in rat KCC2 was removed by silent muta…

Promoter

UbC

Available since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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