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pBiT3.1-N_FBXW12:1-394

Plasmid

#211208

Purpose

Mammalian expression of full-length human FBXW12 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW12 :M1-T394 (FBXW12 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_CORO1B:1-489

Plasmid

#211196

Purpose

Mammalian expression of full-length human CORO1B. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

CORO1B:M1-A489 (CORO1B Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_KIF21A:1337-1653

Plasmid

#211213

Purpose

Mammalian expression of human KIF21A fragment L1337 to A1653. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

KIF21A:L1337-A1653 (KIF21A Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW10:1-1061

Plasmid

#211207

Purpose

Mammalian expression of full-length human FBXW10 isoform 2. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW10:M1-I1061 (FBXW10 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

isoform 2. R275L annotated sequence conflict

Promoter

CMV

Available since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF1:1-1507

Plasmid

#211028

Purpose

Mammalian expression of full-length human DCAF1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:M1-E1507 (DCAF1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_FBXW5:1-566

Plasmid

#211056

Purpose

Mammalian expression of full-length human FBXW5. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

FBXW5:M1-R566 (FBXW5 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_DCAF1:1038-1400

Plasmid

#211052

Purpose

Mammalian expression of human DCAF1 fragment E1038 to E1400. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al bioRxiv 2024.03.03.583197

Insert

DCAF1:E1038-E1400 (DCAF1 Human)

Use

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

wild type

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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Rtn4a-GFP

Plasmid

#61807

Purpose

Human Rtn4a fluorescently tagged with GFP on the C-terminus for mammalian expression

Depositor

Gia Voeltz

Article

Shibata et al J Biol Chem. 2008 Jul 4. 283(27):18892-9

Insert

Rtn4a (RTN4 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Rtn4a Stop Codon removed

Promoter

CMV

Available since

Feb. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pICE-RNaseHI-WT-NLS-mCherry

Plasmid

#60365

Purpose

Plasmid for expression of bacterial RNase HI tagged with NLS-mCherry that can be used to degrade R-loops. Confers resistance to Puromycin. Expression is inducible in T-REx cells.

Depositor

Sébastien Britton, Patrick Calsou

Article

Britton et al Nucleic Acids Res. 2014 Oct 1;42(14):904

Insert

RNase HI

Use

Tags

NLS from SV40 T antigen and mCherry

Expression

Mammalian

Mutation

Promoter

CMV-tet

Available since

Jan. 8, 2015

Availability

Academic Institutions and Nonprofits only

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pH-nCas9-PPE

Plasmid

#140447

Purpose

For plant prime editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

Insert

nCas9(H840A)-M-MLV

Use

CRISPR

Tags

Expression

Plant

Mutation

H840A for Cas9; D200N, T306K, W313F, T330P and L…

Promoter

maize Ubiquitin-1, OsU3

Available since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTol2CG2-ubi:QFGal4-SV40pA

Plasmid

#155119

Purpose

Tol2 vector for ubiquitous expression of QFGal4. Contains cmlc2:mRFP-SV40pA transgenesis marker

Depositor

Bret Pearson

Article

Burgess et al Dev Biol. 2020 Jul 19. pii: S0012-1606(2

Insert

ubi:QFGal4 (ubb Zebrafish, Neurospora crassa, Budding Yeast)

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

ubiquitin B

Available since

Sept. 14, 2020

Availability

Academic Institutions and Nonprofits only

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EGFPC2-CB2 SH3+

Plasmid

#68821

Purpose

EGFP tagged Collybistin expression in mammalian cells

Depositor

Shiva Tyagarajan

Article

de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

Insert

Collybistin (Arhgef9 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Aug. 27, 2015

Availability

Academic Institutions and Nonprofits only

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Cav3P104L-YFP

Plasmid

#68404

Purpose

Expression of fluorescently tagged Caveolin3 point mutation in mammalian cells

Depositor

Rob Parton

Article

Pol et al Mol Biol Cell. 2005 Apr;16(4):2091-105.

Insert

Cav3 (Cav3 Mouse)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Proline at position 104 changed to a Leucine

Promoter

CMV

Available since

Sept. 21, 2015

Availability

Academic Institutions and Nonprofits only
pCytERM-mGreenLantern

Plasmid

#164467

Purpose

Visualization of the ER in cells (compatible with OSER assay)

Depositor

Gregory Petsko

Article

Campbell et al Proc Natl Acad Sci U S A. 2020 Dec 1;117

Insert

mGreenLantern

Use

Tags

Cytochrome p450 first 29 animo acids (Oryctolagus…

Expression

Mammalian

Mutation

Clover-F64L/S72A/E124V/N149K/I167T/S175G/A206K/L2…

Promoter

CMV

Available since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pH-ABE8e-spG

Plasmid

#200775

Purpose

For plant adenine base editing in rice plants or monocotyledons protoplasts

Depositor

Caixia Gao

Article

Xue et al Nat Biotechnol. 2023 Mar 9. doi: 10.1038

Insert

nCas9-spG(D10A)-TadA8e

Use

CRISPR

Tags

Expression

Plant

Mutation

D10A, D1135L, S1136W, G1218K, E1219Q, R1335Q, T13…

Promoter

maize Ubiquitin-1, OsU3

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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EGFPC2-CB2 SH3-

Plasmid

#68822

Purpose

EGFP tagged Collybistin expression in mammalian cells

Depositor

Shiva Tyagarajan

Article

de Groot et al PLoS Genet. 2017 Oct 25;13(10):e1007073.

Insert

Collybistin (Arhgef9 Rat)

Use

Tags

EGFP

Expression

Mammalian

Mutation

none

Promoter

CMV

Available since

Aug. 26, 2015

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R/K63R)-GFP

Plasmid

#212795

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R/K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R/K63R)-GFP

Use

Integrative vector

Tags

Expression

Yeast

Mutation

CUb(K48R/K63R)

Promoter

CUP1

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDF1-ADAM29 (E111K)

Plasmid

#31144

Depositor

Yardena Samuels

Article

Wei et al Hum Mutat. 2011 Jun;32(6):E2148-75. doi:

Insert

ADAM29 (ADAM29 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Glutamic acid 111 changed to Lysine

Promoter

Available since

Sept. 1, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA5-FRT-TO-EGFR*-CUbo-GFP

Plasmid

#212815

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-GFP in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Use

Tags

8xHis and CUbo-GFP

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-EGFR*-CUbo-His6

Plasmid

#212819

Purpose

Constitutive or doxycycline-inducible expression of EGFR*-CUbo-His6 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

EGFR* (EGFR Human, Budding Yeast)

Use

Tags

CUbo-6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2024

Availability

Academic Institutions and Nonprofits only

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