Addgene

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pEPCTΩSP0017

Plasmid

#187565

Purpose

Module for constitutive expression of firefly luciferase and nanoluc luciferase driven by 35s promoters

Depositor

Nicola Patron

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Firefly Luciferase

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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GB2513

Plasmid

#187803

Purpose

Module for constitutive expression of dCas9:EDLL, Ms2:VPR and a gRNA targeting the DFR promoter.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

dCasEV2.1

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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GB1024

Plasmid

#187804

Purpose

Module for the constitutive expression of the delta-11-desaturase from Amyelois transitella and a fatty acid reductase from Helicoverpa armigera.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Atr delta11

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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GB3683

Plasmid

#187808

Purpose

Transcriptional unit for expression of 1,2-diacyl-sn-glycerol:acetyl-CoA acetyltransferase from Euonymus fortunei, codon optimized for Nicotiana.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

EfDAct

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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GB3897

Plasmid

#187809

Purpose

Module for dCasEV2.1 activated expression of AtrD11, HarFAR and ScATF1 plus gRNA-1DFR.

Depositor

Diego Orzaez

Article

Kallam et al Plant Biotechnol J. 2023 Apr 9. doi: 10.

Insert

Moth Pheromone pathway

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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pMXBP_aFb

Plasmid

#114262

Purpose

POC12404, MetClo assembly vector with F replication origin and ampicillin resistance for BsaI-based MetClo, adaptor sequence type a-b

Depositor

Christopher A. O'Callaghan

Article

Lin et al Nucleic Acids Res. 2018 Jul 9. pii: 5050

Insert

LacZalpha

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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LentiSAMPHv2

Plasmid

#167934

Purpose

All-in-one lentiviral CRISPRa gRNA expression with blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pGTQL1211YN

Plasmid

#61704

Purpose

Gateway compatible BiFC vector (pEarleyGate201-YN)

Depositor

Yuhai Cui

Article

Lu et al Plant J. 2010 Jan;61(2):259-70. doi: 10.

Type

Empty backbone

Use

Bifc assay

Tags

YFP-N and HA

Expression

Plant

Mutation

Promoter

35S

Available since

May 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-mMaroon1

Plasmid

#83840

Purpose

Expresses mMaroon1

Depositor

Michael Lin

Article

Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mMaroon1

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 3, 2016

Availability

Academic Institutions and Nonprofits only

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SpyCatcher003-sfGFP

Plasmid

#133449

Purpose

Expresses SpyCatcher003-superfolder GFP in bacterial cytoplasm

Depositor

Mark Howarth

Article

Keeble et al Proc Natl Acad Sci U S A. 2019 Dec 10. p

Insert

SpyCatcher003-sfGFP

Use

Tags

His6 and TEV site

Expression

Bacterial

Mutation

Promoter

T5

Available since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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mCyRFP1-Cdc42

Plasmid

#84360

Purpose

fusion protein of Cdc42, FRET donor

Depositor

Ryohei Yasuda

Article

Laviv et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

Insert

mCyRFP1-Cdc42

Use

Tags

mCyRFP1 N terminal fusion to Cdc42

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1ER

Plasmid

#36325

Depositor

Roger Tsien, Amy Palmer

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1ER second generation cameleon (calcium sensor) targeted to ER

Use

Tags

KDEL

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 12, 2012

Availability

Academic Institutions and Nonprofits only

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pAM5409

Plasmid

#132662

Purpose

RSF1010-based broad host-range plasmid with RK2 bom site expressing YFP and Sp/Sm resistance

Depositor

Susan Golden

Article

Bishe et al iScience. 2019 Oct 25;20:216-228. doi: 1

Inserts

aadA
YFP

Use

Tags

Expression

Bacterial

Mutation

Promoter

PaadA and PconII

Available since

Nov. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCasper_Pdf-LexA::VP16

Plasmid

#33259

Depositor

Michael Rosbash

Article

Shang et al Proc Natl Acad Sci U S A. 2008 Dec 16;10

Insert

LexA::VP16-SV40 3'UTR (lexA E. coli)

Use

Tags

Expression

Insect

Mutation

Promoter

Pdf

Available since

Dec. 12, 2011

Availability

Academic Institutions and Nonprofits only
LentiGuide-TagBFP-2A-BSD

Plasmid

#167929

Purpose

Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6, EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pLK65

Plasmid

#37257

Depositor

Ann Hubbard

Article

Kallay et al J Cell Biochem. 2006 Oct 1;99(2):647-64.

Insert

vang-like 2 (VANGL2 Human)

Use

Adenoviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only
TfR-pHuji

Plasmid

#61505

Purpose

Human transferrin receptor tagged with pH sensitive red fluorescent protein pHuji

Depositor

David Perrais

Article

Shen et al J Cell Biol. 2014 Nov 10;207(3):419-32.

Insert

Transferrin receptor (TFRC Human)

Use

Tags

pHuji

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2014

Availability

Academic Institutions and Nonprofits only

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pJJB302

Plasmid

#107694

Purpose

OsActin Promoter-LwaCas13a-HSP Terminator

Depositor

Daniel Voytas

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

Cas13a

Use

Tags

Expression

Plant

Mutation

Promoter

OsActin

Available since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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LES2A-CRE

Plasmid

#167933

Purpose

Lentiviral CRE expression tracked by mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Insert

mScarlet-P2A-CRE

Use

Cre/Lox and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-D1cpV

Plasmid

#37479

Depositor

Roger Tsien, Amy Palmer

Article

Palmer et al Proc Natl Acad Sci U S A. 2004 Dec 14;10

Insert

D1cpV second generation cameleon (calcium sensor)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 11, 2012

Availability

Academic Institutions and Nonprofits only