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pPyCAG-HA-EGFP-Myc-TM-IRES-Pac
Plasmid#183605PurposeMammalian expression vector for membrane-tethered HA- and Myc-tagged EGFP from the CAG promoter. Confers puromycin resistance.DepositorInsertEGFP-TM
UseTagsHA, Human PDGFRB transmembrane domain, Mouse IgGK…ExpressionMammalianMutationPromoterCAGAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pOPINK-TRABID (NZF1, aa 1-33)
Plasmid#65437PurposeExpresses K29-/K33-specific TRABID NZF1 (aa 1-33) in E. coliDepositorInsertTRABID (ZRANB1 Human)
UseTagsHis6-GSTExpressionBacterialMutationonly codes for amino acids 1-33 of TRABID, which …PromoterT7Available sinceJuly 27, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRDA_791
Plasmid#216079PurposeMinimal plasmid; contains only ori and ampR, destination vectorDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseDestinationTagsExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA295
Plasmid#215986PurposeFragmid fragment: (N' terminus) SV40 Nuclear Localization SignalDepositorHas ServiceCloning Grade DNAInsertStart; SV40NLS_v1.19
UseFragmentTagsExpressionMutationPromoterAvailable sinceMay 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA014
Plasmid#216057PurposeFragmid fragment: (2A Selection) Puromycin resistanceDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; PuroR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA032
Plasmid#216012PurposeFragmid fragment: (Cas protein) deactivated CasDepositorHas ServiceCloning Grade DNAInsertdCas9_v1.1 [Sp]
UseCRISPR; FragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA065
Plasmid#215965PurposeFragmid fragment: (Pol 2 promoter) human EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEF1a_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGL-p21UTR
Plasmid#20014DepositorInsertp21 3'UTR (Cdkn1a Mouse)
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
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pCI-neo.ARRDC1
Plasmid#38321DepositorInsertARRDC1 (ARRDC1 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 15, 2012AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA3.1-CD63-(TEV)-tTA
Plasmid#162597Purposehuman CD63-TEV cleavage site-tTADepositorInserthuman CD63 (CD63 Human)
UseTagsTEV protease cleavage site and tTAExpressionMammalianMutationPromoterCMV promoterAvailable sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
FGF10-luciferase
Plasmid#24994DepositorInsertFgf10 promoter (Fgf10 Mouse)
UseLuciferaseTagsluciferaseExpressionMammalianMutationPromoterAvailable sinceAug. 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
XE122 XDsh delta DIX-GFP-CS2+
Plasmid#16787DepositorInsertXdsh-delta DIX (dvl2.L Frog)
UseXenopus expressionTagsgfpExpressionMutationdeletion of aa 1-159PromoterAvailable sinceMarch 14, 2008AvailabilityAcademic Institutions and Nonprofits only -
pBluescriptII[attB/Ins1]
Plasmid#74100Purpose"intermediate vector" for the two-step cloning method (Wang and Szaro, Dev Biol, 2015)DepositorInsertsPhi C31 attb element
Dual chicken beta-globin hypersensitive site 4 (HS4) insulators
UseTagsExpressionBacterialMutationPromoterAvailable sinceMay 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
mcherry-p62 Double Mutation/Silent
Plasmid#187982PurposeK7A/D69A p62 double mutant (PB1AA). Internal reference: SMC532DepositorInsertp62 (NUP62 Human)
UseTagsmCherryExpressionMammalianMutationD7A, D69A, silent mutationPromoterAvailable sinceOct. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+) neo with Ywhag CDS
Plasmid#61799PurposepcDNA3.1(+) with full length mouse Ywhag CDSDepositorInsertYwhag CDS (Ywhag Mouse)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pQE80l B-CN-B
Plasmid#196477PurposeProtein expression of SpyCatcher-ELP-CarHCN-ELP-SpyCatcher, with TEV cleavage site and MMP cleavage site respectively before and after each SpyCatcher, and GRGD site linked with (GGGGS)2 before CarHCNDepositorInsertB-CN-B
UseTagsExpressionBacterialMutationPromoterT7Available sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFRTpsiCHECK
Plasmid#154454PurposeExpression of renilla luciferase and firefly luciferaseDepositorInsertRenilla luciferase, firefly luciferase
UseTagsExpressionMutationPromoterAvailable sinceAug. 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pXNgate21-3HA
Plasmid#105078PurposeYeast mating-based Split Ubiquitin Assay, prey vector for in vivo cloningDepositorTypeEmpty backboneUseTagsNubGExpressionYeastMutationPromoterAvailable sinceFeb. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCAG-H2B-mRuby3-IRES-eGtACR1-ST
Plasmid#108960PurposeAnion channelrhodopsin GtACR1 fused to ER export signal and targeted to the neuronal soma and proximal dendrites under the control of internal ribosome entry sequenceDepositorInserteGtACR1-ST
UseTagsExpressionMammalianMutationPromoterAvailable sinceMay 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFastBac(His10-SNAP-Csk)
Plasmid#177895PurposeBaculo expression of His10 tag fused to SNAPtag and human CskDepositorInsertCsk (CSK Human)
UseTags10xHis-TEV-TVMV-SNAP tagExpressionInsectMutationPromoterPolyhedrinAvailable sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
STAGR_gRNAScaffold_hH1
Plasmid#102841PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert gRNAScaffold_hH1
UsePcr template for stagr insertsTagsExpressionMutationPromoterhH1Available sinceMay 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
TB:7xSMAD_RE:MiniP
Plasmid#133879Purpose7 copies the SMAD DNA binding motifDepositorInsert7 copies the SMAD DNA binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pDRf1-4CL5-DsRed (JBEI-15461)
Plasmid#87935PurposeCloning vector for co-expressing a gene with 4CL5 in S.cerevisiaeDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
AA015
Plasmid#216058PurposeFragmid fragment: (2A Selection) Puromycin resistance, T2A, EGFPDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; PuroR_v1.1; T2A_v1.2; EGFP_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA041
Plasmid#215963PurposeFragmid fragment: (Pol 2 promoter) human core (short) EF1a promoterDepositorHas ServiceCloning Grade DNAInsertEFS_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA406
Plasmid#216070PurposeFragmid fragment: (2A Selection) Violet-excited GFP, T2A, blasticidin resistanceDepositorHas ServiceCloning Grade DNAInsertP2A_v1.1; VexGFP_v1.1; T2A_v1.1; BlastR_v1.1; Stop
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(-)2G
Plasmid#62799PurposemtZFN2G architecture bearing (-) FokI domainDepositorInsertmtZFN(-)2G
UseTagsInternal FLAG tag immediately after F1B mitochond…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-)_mtZFN(+)2G
Plasmid#62798PurposemtZFN2G architecture bearing (+) FokI domainDepositorInsertmtZFN(+)2G
UseTagsInternal HA tag immediately after F1B mitochondri…ExpressionMammalianMutationPromoterCMVAvailable sinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
7xSMAD_RE::GrRenilla
Plasmid#178319PurposeSMAD GreenRenilla luciferase reporterDepositorInsertSMAD GreenRenilla Reporter
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
mRIP3 GFP D161N
Plasmid#41383DepositorInsertRIP3 (Ripk3 Mouse)
UseTagsGFPExpressionMammalianMutationKinase Domain Mutant (D161N)PromoterCMVAvailable sinceDec. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
TB:6xAP-1_RE:MiniP
Plasmid#133884Purpose6 copies of the AP-1 binding motifDepositorInsert6 copies of the AP-1 binding motif
UseLuciferase and Synthetic BiologyTagsExpressionMammalianMutationPromoterAvailable sinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLacI-LAP-BUB1
Plasmid#115905PurposeUsed to express BUB1-WT fused to LacI for recruitment of BUB1 to LacO spots.DepositorInsertLacI-BUB1 (BUB1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
STAGR_SAMScaffold_mU6
Plasmid#102845PurposeCan be used as PCR template for a STAgR reactionDepositorInsertSTAgR Insert SAM_mU6
UsePcr template for stagr insertsTagsExpressionMutationPromotermU6Available sinceNov. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pFBD-Akt1(WT)
Plasmid#86561PurposeExpresses human full-length Akt1(WT)DepositorInsertAkt1 (AKT1 Human)
UseTags10xHisExpressionInsectMutationPromoterAvailable sinceMarch 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pP19 (GB0038)
Plasmid#68195PurposeProvides the CDS of the Tomato bushy stunt virus (TBSV) silencing suppressor P19 as a level 0 GoldenBraid partDepositorInsertP19 coding region
UseSynthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceOct. 28, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-DLL1
Plasmid#205201PurposeThis vector encodes of the synCAM (DLL-1) and GFP nanobody LAG16DepositorInsertsynCAM DLL1 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR- flag-Lag16-MUC4
Plasmid#205198PurposeThis vector encodes of the synCAM (MUC4) and GFP nanobody LAG16DepositorInsertsynCAM MUC4 and Lag16 anti-GFP nanobody
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
px459-RhebL1 sgRNA
Plasmid#133769PurposeExpresses Cas9 and human RhebL1 sgRNADepositorInsertRhebL1 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceNov. 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pMetYCgate
Plasmid#105074PurposeYeast mating-based Split Ubiquitin Assay, bait vector for in vivo cloningDepositorTypeEmpty backboneUseTagsCub-PLVExpressionYeastMutationPromoterAvailable sinceFeb. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
XE49 pt Beta-catenin-myc
Plasmid#16840DepositorInsertpt beta-catenin-myc (ctnnb1.L Frog)
UseXenopus expressionTags6x mycExpressionMutationfour point mutations introduced into the GSK-3 ph…PromoterAvailable sinceJune 25, 2009AvailabilityAcademic Institutions and Nonprofits only -
pRS423-FjTAL(JBEI-15466)
Plasmid#87936PurposeExpression in S.cerevisiae of FjTALDepositorInsertFjTAL
UseTagsExpressionYeastMutationPromoterAvailable sinceMay 3, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTS2410-Tier1-PhCMV-Myr_mTagBFP2
Plasmid#169535PurposeTier-1 vector encoding PhCMV-controlled plasma membrane-tethered mTagBFP2 (PhCMV-myr-mTagBFP2-pA).DepositorInsertPCMV-driven membrane-localized monomeric Tag blue fluorescent protein 2
UseTagsExpressionMammalianMutationPromoterPhCMVAvailable sinceJune 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Dest47- C11orf83-GFP
Plasmid#65844PurposeMammalian expression of C terminally GFP-tagged C11orf83/UQCC3DepositorInsertUQCC3 WT (UQCC3 Human)
UseTagsGFPExpressionMammalianMutationWTPromoterAvailable sinceJuly 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRN3P-HA-Suv4-20h2
Plasmid#86691PurposeFor transcription of of Suv4-20h2 mRNA preceded by an HA-tag in 5'DepositorInserthistone-lysine N-methyltransferase KMT5C (Kmt5c Mouse)
UseTagsHAExpressionMutationPromoterT3Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only
