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pRK5 CASTOR1-Flag Cterm

Plasmid

#84488

Purpose

overexpression of CASTOR1 with Flag tag

Depositor

David Sabatini

Article

Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

Insert

CASTOR1 (GATSL3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 24, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-U6

Plasmid

#19799

Depositor

Zuoshang Xu

Article

Qiu et al BMC Biotechnol. 2008 . 8():77.

Type

Empty backbone

Use

RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 21, 2008

Availability

Academic Institutions and Nonprofits only
ptf-Galectin3

Plasmid

#64149

Purpose

Expression in mammalian cells

Depositor

Tamotsu Yoshimori

Article

Maejima et al EMBO J. 2013 Aug 28;32(17):2336-47. doi:

Insert

Galectin3 (LGALS3 Human)

Use

Tags

EGFP and mRFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 17, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV2-SEP-GluA1 (M1)

Plasmid

#64942

Purpose

mammalian expression of rat GluA1

Depositor

Jeremy Henley

Article

Blanco-Suarez et al J Biol Chem. 2014 Feb 21;289(8):4644-51.

Insert

GluA1 (Gria1 Rat)

Use

Tags

SEP (superecliptic pHluorin)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 14, 2015

Availability

Academic Institutions and Nonprofits only

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pT-GEM(0)

Plasmid

#62891

Purpose

Trojan-Gal4 Expression Module in Phase 0. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

July 2, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-mCherry-IRES-Cre

Plasmid

#55632

Purpose

Expresses Cre in Mammalian Cells

Depositor

Karl Deisseroth

Article

Fenno et al Nat Methods. 2014 Jul;11(7):763-72. doi:

Has Service

AAV Retrograde and AAV8

Inserts

mCherry
Cre

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Ef1a and Ef1a/IRES

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLJM1-Empty

Plasmid

#91980

Purpose

Empty backbone

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pT-GEM(1)

Plasmid

#62893

Purpose

Trojan-Gal4 Expression Module in Phase 1. Insert T2A-Gal4 to genomic loci using the Crispr/Cas technology. Can be converted by cassette exchange into other Trojan exons

Depositor

Benjamin White

Article

Diao et al Cell Rep. 2015 Mar 3;10(8):1410-21. doi:

Type

Empty backbone

Use

Tags

Expression

Mutation

Promoter

Available since

May 1, 2015

Availability

Academic Institutions and Nonprofits only

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pET-6xHis-SUMO-MarathonRT

Plasmid

#109029

Purpose

Group II intron maturase from Eubacterium rectale (wild-type sequence). This is a highly processive reverse transcriptase that is called “MarathonRT”

Depositor

Anna Pyle

Article

Zhao et al RNA. 2018 Feb;24(2):183-195. doi: 10.126

Insert

ERE_01490

Use

Tags

6xHis-SUMO tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-hSynapsin1-RdLight1

Plasmid

#125708

Purpose

Expresses the red genetically encoded dopamine sensor RdLight1 in mammalian neurons.

Depositor

Lin Tian

Article

Patriarchi et al Nat Methods. 2020 Nov;17(11):1147-1155.

Has Service

AAV9

Insert

RdLight1

Use

AAV

Tags

Flag tag

Expression

Mutation

Promoter

hSynapsin1

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG_smFP Myc

Plasmid

#59757

Purpose

encodes multiple copies of an epitope tag Myc

Depositor

Loren Looger

Article

Viswanathan et al Nat Methods. 2015 Jun;12(6):568-76. doi:

Insert

smFP _Myc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Sept. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pNO286-3

Plasmid

#107746

Purpose

Key genes: "mini-CcaS #10", ho1, pcyA, spectinomycin resistance, and medium copy backbone (p15a)

Depositor

Jeffrey Tabor

Article

Ong et al Chembiochem. 2018 Feb 8. doi: 10.1002/cb

Inserts

CcaS
Ho1
PcyA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Deleted AA 223 and 512

Promoter

J23106 and J23108

Available since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-PGK-Venus-Fluc (puro)

Plasmid

#140328

Purpose

Lentivirus for expression of Venus-Fluc (firefly luciferase) fusion protein

Depositor

Roland Friedel

Article

Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

Insert

Venus-Firefly Luciferase (luc2)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

human PGK promoter

Available since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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mEGFP-N1

Plasmid

#54767

Purpose

Localization: N1 Cloning Vector, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Tags

mEGFP

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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mEos3.2-C1

Plasmid

#54550

Purpose

Localization: C1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson, Tao Xu

Article

Zhang et al Nat Methods. 2012 May 13;9(7):727-9. doi

Type

Empty backbone

Use

Tags

mEos3.2

Expression

Mammalian

Mutation

Promoter

Available since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pBBRBB-eGFP

Plasmid

#32549

Depositor

Claudia Schmidt-dannert

Article

Vick et al Appl Microbiol Biotechnol. 2011 Dec;92(6

Insert

eGFP (eGFP )

Use

Synthetic Biology

Tags

His

Expression

Bacterial

Mutation

Promoter

Consitutive lac

Available since

Nov. 8, 2011

Availability

Academic Institutions and Nonprofits only

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pJCSUP35

Plasmid

#1088

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Use

Tags

HIS tag

Expression

Bacterial

Mutation

Promoter

Available since

May 26, 2005

Availability

Academic Institutions and Nonprofits only
pCDH-CMV-mC-G-LC3B-P

Plasmid

#124974

Purpose

evaluate autophagy

Depositor

Kazuhiro Oka

Article

Sutton et al Autophagy. 2018;14(4):637-653. doi: 10.1

Insert

mCherry-GFP-LC3B

Use

Lentiviral

Tags

Expression

Mutation

Promoter

CMV

Available since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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tau/pET29b

Plasmid

#16316

Depositor

Peter Klein

Article

Hedgepeth et al Dev Biol. 1997 May 1. 185(1):82-91.

Insert

tau (MAPT Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 21, 2007

Availability

Academic Institutions and Nonprofits only

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pNX32-Dest

Plasmid

#105083

Purpose

Yeast mating-based Split Ubiquitin Assay, prey vector for Gateway cloning

Depositor

Christopher Grefen

Article

Grefen et al Curr Protoc Neurosci. 2007 Oct;Chapter 5

Type

Empty backbone

Use

Tags

NubG

Expression

Yeast

Mutation

Promoter

Available since

Feb. 14, 2018

Availability

Academic Institutions and Nonprofits only

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