-
Plasmid#141274PurposeGateway-compatible Entry vector, with insert of the membrane (M) gene's CDS from SARS-CoV-2 isolate Wuhan-Hu-1DepositorUseGateway-compatible entry vectorTagsExpressionMutationMany synonymous changes due to codon optimizationPromoterAvailable sinceApril 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits
-
pMK291 (mAID-mClover-Bsr)
Plasmid#72829PurposeC-terminal tagging with mAID-mClover using CRISPR/Cas9DepositorInsertmAID-mClover-Bsr
UseCRISPRTagsmAID-mCloverExpressionMammalianMutationPromoterAvailable sinceFeb. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
-
AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato
Plasmid#51083PurposeCre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophoreDepositorHas ServiceAAV RetrogradeInsertGCaMP6f
UseAAVTagsP2A-nls-dTomatoExpressionMammalianMutationPromoterhEF1aAvailable sinceFeb. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
Flag-HA-BAP1
Plasmid#22539DepositorInsertBAP1 (BAP1 Human)
UseRetroviralTagsFLAG and HAExpressionMammalianMutationNonePromoterAvailable sinceNov. 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
MSCV JMJD3
Plasmid#21212DepositorInsertJMJD3 (KDM6B Human)
UseRetroviralTagsFlag and HAExpressionMammalianMutationPromoterAvailable sinceMay 22, 2009AvailabilityAcademic Institutions and Nonprofits only -
Stat3 Flag pRc/CMV
Plasmid#8707DepositorInsertStat3 (Stat3 Mouse)
UseTagsFlagExpressionMammalianMutation*see commentsPromoterAvailable sinceSept. 26, 2005AvailabilityAcademic Institutions and Nonprofits only -
pX330-Cetn1/1
Plasmid#50718PurposepX330 containing sgRNA against mouse Cent1. Positive control for DSB mediated EGFP reconstitution.DepositorInsertsCetn1 sgRNA1
humanized S. pyogenes Cas9
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCBh and hU6Available sinceFeb. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHAGE-TO-nls-st1dCas9-3nls-3XTagBFP2
Plasmid#64512PurposeExpression of St1 dCas9-3XTagBFP2 in mammalian cellsDepositorInsertSt1 dCas9
UseCRISPR and LentiviralTags3XmTagBFP2ExpressionMammalianMutationPromoterCMV-TOAvailable sinceMay 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBABE-bleo IGF-1R
Plasmid#11212DepositorInsertIGF-1R (IGF1R Human)
UseRetroviralTagsExpressionMammalianMutationPromoterAvailable sinceMay 24, 2006AvailabilityAcademic Institutions and Nonprofits only -
pCMV-HA-MyD88
Plasmid#12287DepositorInsertMyD88 (MYD88 Human)
UseTagsHAExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2007AvailabilityAcademic Institutions and Nonprofits only -
phASCL1-N106
Plasmid#31781DepositorInsertachaete-scute complex homolog 1 (ASCL1 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceSept. 13, 2011AvailabilityAcademic Institutions and Nonprofits only -
pBabe-Puro-MEK-DD
Plasmid#15268DepositorInsertMap2k1 (Mapk1 Mouse)
UseRetroviralTagsExpressionMammalianMutationS218D S222DPromoterAvailable sinceJuly 12, 2007AvailabilityAcademic Institutions and Nonprofits only -
pdCas9-DNMT3A-PuroR
Plasmid#71667PurposeExpression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNADepositorInsertS. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Synthetic, S. pyogenes, Human)
UseCRISPRTags3xFLAG, SV40 NLS, and T2A-PuroRExpressionMammalianMutationD10A and H840A in S.pyogenes Cas9PromoterCBhAvailable sinceMarch 7, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 HA Arf6 ActQ67L
Plasmid#10835DepositorInsertArf6 Q67L (ARF6 Human)
UseTagsHAExpressionMammalianMutationQ67L. Defective in GTP hydrolysis. Constitutive…PromoterAvailable sinceApril 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
FUW-tetO-hKLF4
Plasmid#20725DepositorInsertKLF4 (KLF4 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceMarch 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
Ubc.Luc.IRES.Puro
Plasmid#33307DepositorInsertFirefly Luciferase
UseLentiviral and LuciferaseTagsExpressionMammalianMutationPromoterUbcAvailable sinceFeb. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
PX404 Campylobacter jejuni Cas9
Plasmid#68338PurposeCampylobacter jejuni Cas9DepositorInsertCjCas9
UseCRISPRTagsHA-NLS and NLSExpressionMammalianMutationPromoterCMVAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFRT/TO/HIS/FLAG/HA-ALKBH5
Plasmid#38073DepositorInsertALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
UseTagsHIS/FLAG/HAExpressionMammalianMutationcDNA clone MGC:71143 IMAGE:6339184 with longer 3&…PromoterAvailable sinceSept. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -