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Plasmid#154885PurposepiggyBac self-reporting transposon with tdTomato marker for mammalian calling cardsDepositorInsertpiggyBac SRT
UseTransposonTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBRPB CAG-mCherry-IP
Plasmid#106333PurposePiggyBac transposon, mCherry-IRES-puro labeling under the control of CAG promoter. Used in chimera experiment of ES cells.DepositorInsertmCherry-IRES-Puro
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-Neo-TetO-Cdk12_Untagged
Plasmid#127178PurposeUntagged mouse Cdk12 transgene (NM_001109626.1 isoform) cloned into a dox-inducible piggybac destination vectorDepositorInsertMouse Cyclin Dependent Kinase 12 (Cdk12 Mouse)
UsePiggybac transposase vectorTagsNoneExpressionMammalianMutationPromoterTetO PromoterAvailable sinceJuly 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
138-dCas9-Dnmt3a
Plasmid#84570PurposePiggyBac transposon system construct with dCas9-Dnmt3aDepositorInsertdCas9-Dnmt3a (DNMT3A Synthetic, Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 25, 2017AvailabilityAcademic Institutions and Nonprofits only -
PB-CRX-T2A-NeuroD1
Plasmid#194607PurposePiggybac Tet-ON plasmid for inducing hiPSCs into photoreceptor-like cells via CRX and NeuroD1 expression.DepositorInsertCRX-T2A-NeuroD1
UsePiggybac transposonTagsExpressionMammalianMutationPromotertetOAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLR5-CAG-hMCP-mScarlet-I-NLS
Plasmid#174878PurposePiggyBac transposon vector for expression of MCP-mScarlet-I-NLSDepositorInsertMCP-mScarlet-I-NLS
UsePiggybac vectorTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-SRT-Puro
Plasmid#154884PurposepiggyBac self-reporting transposon with puromycin marker for mammalian calling cardsDepositorInsertpiggyBac SRT
UseTransposonTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEF1α-M2rtTA-T2A-Puro
Plasmid#169175PurposepiggyBac co-transfection transposon containing M2rtTA promoter linked with Puromycin resistance gene, through T2A with GSG linker, subcloned between ends of two ITRs. Pairs with #169174DepositorInsertM2rtTA-T2A-Puro
UsePiggybac transposonTagsExpressionMammalianMutationSee Depositor Comments BelowPromoterEF1aAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-Cas9
Plasmid#120354PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [1-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
mCh-LgBiT-2A-EGFP-Vin-PILATeS
Plasmid#216761PurposePiggyBac vector for stable co-expression of mCherry-LgBiT and EGFP-Vinculin-PILATeS (700 pM) in mammalian cells. Requires transposaseDepositorInsertmCherry-LgBiT-P2A-T2A-EGFP-Vin-PILATeS
UseTagsEGFP and mCherryExpressionMammalianMutationPromoterAvailable sinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
PB-TAC-OK+9MS-KRAB-dCas9
Plasmid#120357PurposepiggyBac transposon for dox-inducible expression of the OK+9MS (Oct4, Klf4[1-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). KRAB-dCas9 is constitutively expressed.DepositorInsertOK+9MS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [1-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
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PB-UBC-GCAMP6f-PGK-neo
Plasmid#160049Purposeexpression of GCaMP6f in a piggybac plasmidDepositorInsertGCaMP6f
UseTagsExpressionMammalianMutationPromoterUbcAvailable sinceMay 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTA3G-IH
Plasmid#214118PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInserttrTA3G
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
BII-tdT-inv-GFP
Plasmid#133393PurposePiggybac vector for bidirectional promoter reporter assayDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterNo promoterAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPB-CAG-rtTAM2-IB
Plasmid#214117PurposeGene expression by Piggybac transposase in mammalian cellsDepositorInsertrtTAM2
UseTagsExpressionMammalianMutationCodon-optimized for mammalian cellsPromoterAvailable sinceFeb. 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-smURFP
Plasmid#133399PurposePiggybac vector for constitutive expression of smURFP-NLSDepositorInsertsmURFP-NLS
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-STAR-tdT
Plasmid#133396PurposePiggybac vector for ASCL2 reporterDepositorInserttdTomato-NLS
UseTagsExpressionMammalianMutationN/APromoterStem Cell Specific ASCL2 response elements with L…Available sinceFeb. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-ChPtW-iRFP702
Plasmid#133398PurposePiggybac vector for constitutive expression of iRFP702DepositorInsertIRFP-702
UseTagsExpressionMammalianMutationN/APromoterCMV enhancer and hEf1a (CpG free)Available sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only